AB Sciex QTRAP 4500 LC/MS/MS system System User's Manual page 83

Table 10-5 Summary of XIC Generation Methods (Continued)
Method Use with
Chromatogram
Base peak Yes
masses
Specified masses Yes
Prerequisite Procedure
• Open Data Files on page
Tip! To see an example data file, make sure that the Example project is selected.
Open the LIT folder, and then open the Reserpine.wiff file.
Generate an XIC Using a Selected Range
1. Select a range by clicking and holding the left mouse button at the start of the range
and then dragging the cursor to the stop point and release.
The selection is highlighted in blue.
2. Click Explore > Extract Ions > Use Range.
An XIC of the specified selection opens in a pane below the spectrum pane. The
experiment information at the top of the pane contains the mass range and the
maximum intensity in counts per second.
Generate an XIC Using the Maximum Peak
1. Select a range.
The selection is highlighted in blue.
2. Click Explore > Extract Ions > Use Maximum.
An XIC of the maximum peak specified selection opens below the spectrum pane.
The experiment information at the top of the pane contains the mass range and the
maximum intensity in counts per second.
Generate an XIC Using Base Peak Masses
1. In a BPC, select the peak from which to extract ions.
The selection is highlighted in blue.
System User Guide
RUO-IDV-05-0264-A
Use with
Spectrum
No
Yes
77. Open a data file containing spectra.
Operating Instructions — Analyze and Process Data
Extraction
Can be used only with BPCs (Base
Peak Chromatograms.) Use the Use
Base Peak Masses command to
extract ions results in an XIC with a
different colored trace for each mass.
If the selection includes multiple
peaks, the resulting XIC will have an
equal number of colored traces
representing each mass.
Extracts ions from any type of
spectrum or chromatogram. Select up
to ten start and stop masses for which
to generate XICs.
4500 Series of Instruments
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