Setting Up An Analysis; Before Using The System - Agilent Technologies 1260 User Manual

5
Using the Detector

Setting up an Analysis

Setting up an Analysis

This chapter may be used to
• Prepare the system,
• Get to know the set up of an HPLC analysis and
• Use it as an instrument check to demonstrate that all modules of the system
• Learn about special settings

Before Using the System

Solvent Information
Observe recommendations on the use of solvents in chapter "Solvents".
Priming and Purging the System
When the solvents have been exchanged or the pumping system has been
turned off for a certain time (for example, overnight) oxygen will re-diffuse
into the solvent channel between the solvent reservoir, vacuum degasser
(when available in the system) and the pump. Volatile ingredients will
evaporate to some extend. Therefore priming of the pumping system is
required before starting an application.
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are correctly installed and connected. It is not a test of the instrument
performance.
Agilent 1260 Infinity DAD and MWD User Manual