Intensity Test - Agilent Technologies 1260 User Manual

9
Test Functions

Intensity Test

Intensity Test

The test is for the standard flow cells (10 mm and 6 mm pathlength) only. The nano-flow
N O T E
cells (80 nl and 500 nl) cannot be run with this test due to its low volume.
The intensity test measures the intensity of the deuterium and tungsten lamps
over the full wavelength range (190 – 950 nm). Four spectral ranges are used
to evaluate the intensity spectrum. The test is used to determine the
performance of the lamps and optics (see also
the test is started, the 1-nm slit is moved into the light path automatically, and
the gain is set to zero. To eliminate effects due to absorbing solvents, the test
should be done with water in the flow cell. The shape of the intensity
spectrum is primarily dependent on the lamp, grating, and diode array
characteristics. Therefore, intensity spectra will differ slightly between
instruments.
170
Figure 67 on page 171 shows a typical intensity test spectrum.
"Cell Test"
Agilent 1260 Infinity DAD and MWD User Manual
on page 179). When