Agilent Technologies 1260 Infinity User Manual
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Agilent Technologies 1260 Infinity User Manual

Isocratic pump and quaternary pump
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  • Page 1 Agilent 1260 Infinity Isocratic Pump and Quaternary Pump User Manual Agilent Technologies...
  • Page 2 Notices Warranty © Agilent Technologies, Inc. 2010 receive no greater than Restricted Rights as defined in FAR 52.227-19(c)(1-2) (June No part of this manual may be reproduced The material contained in this docu- 1987). U.S. Government users will receive in any form or by any means (including elec- ment is provided “as is,”...

  • Page 3: Table Of Contents

    Contents Contents 1 Introduction Introduction to the Pump Overview of the Hydraulic Path Early Maintenance Feetback Instrument Layout Electrical Connections Interfaces Setting the 8-bit Configuration Switch 2 Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications 3 Installing the Pump Unpacking the Pump Optimizing the Stack Configuration Installing the Pump...
  • Page 4 Contents 6 Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Status Indicators User Interfaces Agilent Lab Advisor Software 7 Error Information What Are Error Messages General Error Messages Module Error Messages 8 Test Functions and Calibration Introduction System Pressure Test Leak Rate Test 9 Maintenance...
  • Page 5 External Contact Cable Agilent Module to PC Agilent 1200 Module to Printer 12 Appendix General Safety Information The Waste Electrical and Electronic Equipment Directive Batteries Information Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet Agilent 1260 IsoPump/QuatPump User Manual...
  • Page 6 Contents Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 7: Introduction

    Electrical Connections Serial Number Information (ALL) Rear View of the Module Interfaces Overview Interfaces Setting the 8-bit Configuration Switch Communication Settings for RS-232C Special Settings This chapter gives an introduction to the module, instrument overview and internal connectors Agilent Technologies...

  • Page 8: Introduction To The Pump

    Introduction Introduction to the Pump Introduction to the Pump Introduction to the Quaternary Pump The quaternary pump comprises a solvent cabinet, a vacuum degasser and a four-channel gradient pump. The four-channel gradient pump comprises a high-speed proportioning valve and a pump assembly. It provides gradient generation by low pressure mixing.
  • Page 9 Introduction Introduction to the Pump Introduction to the Isocratic Pump The isocratic pump has the same operating principle as the quaternary pump but has only one solvent channel, that means the composition cannot be changed during a method because there is no multi-channel gradient valve (MCGV).

  • Page 10: Overview Of The Hydraulic Path

    Introduction Overview of the Hydraulic Path Overview of the Hydraulic Path Both the isocratic pump and quaternary pump are based on a two-channel, dual-plunger in-series design which comprises all essential functions that a solvent delivery system has to fulfill. Metering of solvent and delivery to the high-pressure side are performed by one pump assembly which can generate pressure up to 600 bar.
  • Page 11 Introduction Overview of the Hydraulic Path Hydraulic Path Figure 3 Hydraulic Path of the Isocratic Pump Agilent 1260 IsoPump/QuatPump User Manual...
  • Page 12 Introduction Overview of the Hydraulic Path Figure 4 Hydraulic Path of the Quaternary Pump How Does the Pump Work? In the quaternary pump, the liquid runs from the solvent reservoir through the degasser to the MCGV and from there to the inlet valve. For the isocratic pump, the solvent bottle is directly connected to the inlet valve.
  • Page 13 Introduction Overview of the Hydraulic Path limit and leaves it at its top. The outer diameter of the piston is smaller than the inner diameter of the pump head chamber allowing the solvent to fill the gap inbetween. The first piston has a stroke volume in the range of 20 – 100 µL depending on the flow rate.
  • Page 14 Introduction Overview of the Hydraulic Path When turned on, the pump runs through an initialization procedure to determine the upper dead position of the first piston. The first piston moves slowly upwards into the mechanical stop of the pump chamber and from there it moves back for a defined distance.
  • Page 15 Introduction Overview of the Hydraulic Path Table 2 Quaternary Pump Details Delay volume 800 – 1100 µL, dependent on back pressure Materials in contact with mobile phase MCGV PTFE Pump head SST, gold, sapphire, ceramic Active inlet valve SST, gold, sapphire, ruby, ceramic, PTFE Outlet valve SST, gold, sapphire, ruby Adapter...
  • Page 16 Introduction Overview of the Hydraulic Path With a compressibility value set the processor calculates a compensation volume, that depends on the back pressure of the system and the selected compressibility. This compensation volume will be added to the normal stroke volume and compensates the previously described loss of volume during the delivery stroke of the first piston.

  • Page 17: Early Maintenance Feetback

    Introduction Early Maintenance Feetback Early Maintenance Feetback Maintenance requires the exchange of components which are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions, and not on a predefined time interval.

  • Page 18: Instrument Layout

    Introduction Instrument Layout Instrument Layout The industrial design of the module incorporates several innovative features. It uses Agilent’s E-PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed.

  • Page 19: Electrical Connections

    There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.
  • Page 20 Introduction Electrical Connections Serial Number Information (ALL) The serial number information on the instrument labels provide the following information: CCXZZ00000 Format Country of manufacturing (DE Germany) Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module) 00000 Serial number...

  • Page 21: Interfaces

    Introduction Interfaces Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces: Table 3 Agilent 1200 Infinity Series Interfaces Module LAN/BCD RS-232 Analog Special (optional) (on-board) Remote Pumps G1310B Iso Pump G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump...
  • Page 22 Introduction Interfaces Table 3 Agilent 1200 Infinity Series Interfaces Module LAN/BCD RS-232 Analog Special (optional) (on-board) Remote G4212A/B DAD G1315C DAD VL+ G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD G1362A RID G4280A ELSD EXT Contact AUTOZERO Others G1316A/C TCC G1322A DEG G1379B DEG...
  • Page 23 Introduction Interfaces Overview Interfaces The CAN is inter-module communication interface. It is a 2-wire serial bus system supporting high speed data communication and real-time requirement. The modules have either an interface slot for an LAN card (e.g. Agilent G1369A/B LAN Interface) or they have an on-board LAN interface (e.g. detectors G1315C/D DAD and G1365C/D MWD).
  • Page 24 Introduction Interfaces Table 4 RS-232C Connection Table Direction Function Ground Figure 7 RS-232 Cable Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s main board. Agilent 1260 IsoPump/QuatPump User Manual...
  • Page 25 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
  • Page 26 Introduction Interfaces Table 5 Remote Signal Distribution Signal Description Not used POWER ON (H) All modules connected to system are switched on. Receiver is any module relying on operation of others. READY (H) System is ready for next analysis. Receiver is any sequence controller.

  • Page 27: Setting The 8-Bit Configuration Switch

    Introduction Setting the 8-bit Configuration Switch Setting the 8-bit Configuration Switch Setting the 8-bit Configuration Switch (On-Board LAN) The 8-bit configuration switch is located at the rear of the module. Switch settings provide configuration parameters for LAN, serial communication protocol and instrument specific initialization procedures. All modules with on-board LAN, e.g.
  • Page 28 Introduction Setting the 8-bit Configuration Switch Table 6 8-bit Configuration Switch (with on-board LAN) Mode Function SW 1 SW 2 SW 3 SW 4 SW 5 SW 6 SW 7 SW 8 Link Configuration Init Mode Selection Auto-negotiation 10 MBit, half-duplex 10 MBit, full-duplex 100 MBit, half-duplex 100 MBit, full-duplex...
  • Page 29 • for boot/test modes DIPS 1+2 must be UP plus required mode Switch settings provide configuration parameters for GPIB address, serial communication protocol and instrument specific initialization procedures. With the introduction of the Agilent 1260 Infinity, all GPIB interfaces have been removed. N O T E The preferred communication is LAN.
  • Page 30 Introduction Setting the 8-bit Configuration Switch Table 7 8-bit Configuration Switch (without on-board LAN) Mode Select RS-232C Baudrate Data Parity Bits Reserved Reserved TEST/BOOT RSVD RSVD RSVD The LAN settings are done on the LAN Interface Card G1369A/B. Refer to the N O T E documentation provided with the card.
  • Page 31 Introduction Setting the 8-bit Configuration Switch Table 9 Baudrate Settings (without on-board LAN) Switches Baud Rate Switches Baud Rate 9600 9600 1200 14400 2400 19200 4800 38400 Table 10 Data Bit Settings (without on-board LAN) Switch 6 Data Word Size 7 Bit Communication 8 Bit Communication Table 11...
  • Page 32 Introduction Setting the 8-bit Configuration Switch Special Settings The special settings are required for specific actions (normally in a service case). The tables include both settings for modules – with on-board LAN and without on-board N O T E LAN. They are identified as LAN and no LAN. Boot-Resident Firmware update procedures may require this mode in case of firmware loading errors (main firmware part).
  • Page 33 Introduction Setting the 8-bit Configuration Switch Table 13 Forced Cold Start Settings (without on-board LAN) Mode Select TEST/BOOT No LAN TEST/BOOT Agilent 1260 IsoPump/QuatPump User Manual...
  • Page 34 Introduction Setting the 8-bit Configuration Switch Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 35: Site Requirements And Specifications

    Agilent 1260 IsoPump/QuatPump User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies...

  • Page 36: Site Requirements

    Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 14 on page 39.
  • Page 37 Never operate your instrumentation from a power outlet that has no ground connection. ➔ Never use a power cord other than the Agilent Technologies power cord designed for your region. Use of unsupplied cables WA R N I N G Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury.
  • Page 38 Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment. ➔ Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. Bench Space The module dimensions and weight (see...

  • Page 39: Physical Specifications

    Site Requirements and Specifications Physical Specifications Physical Specifications Table 14 Physical Specifications Type Specification Comments Weight 11 kg (25 lbs) Dimensions (height × 180 x 345 x 435 mm (7.0 x 13.5 x 17 inches) width × depth) Line voltage 100 –...

  • Page 40: Performance Specifications

    Site Requirements and Specifications Performance Specifications Performance Specifications Table 15 Performance Specification Agilent 1260 Infinity Isocratic Pump (G1310B) Type Specification Hydraulic system Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating pistons Setable flow range 0.001 – 10 mL/min, in 0.001 mL/min increments Flow range 0.2 –...
  • Page 41 Site Requirements and Specifications Performance Specifications Table 16 Performance Specification Agilent 1260 Infinity Quaternary Pump (G1311B) Type Specification Hydraulic system Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating pistons Setable flow range 0.001 – 10 mL/min, in 0.001 mL/min increments Flow range 0.2 –...
  • Page 42 Site Requirements and Specifications Performance Specifications Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 43: Installing The Pump

    Connecting Control Software and/or G4208 A Instant Pilot Flow Connections of the Pump Priming the System Initial Priming Regular Priming Changing Solvents This chapter gives information about the preferred stack setup for your system and the installation of your module. Agilent Technologies...

  • Page 44: Unpacking The Pump

    Unpacking the Pump Unpacking the Pump If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. "Defective on arrival" problems C A U T I O N If there are signs of damage, please do not attempt to install the module.
  • Page 45 Accessory Kit (p/n G1311-68755) (see “Accessory Kit G1311-68755” on page 46) Isocratic Pump Checklist Isocratic Pump Checklist Description G1310-64060 Agilent 1260 Infinity Isocratic Pump G1310B 5065-9981 Solvent cabinet, including all plastic parts 9301-1450 Solvent bottle, amber G1311-60003 Bottle-head assembly 5042-2461...

  • Page 46: Accessory Kit G1311-68755

    Installing the Pump Unpacking the Pump Accessory Kit G1311-68755 Accessory Kit G1311-68755 Description 5062-2461 Waste tube, 5 m (reorder pack) 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) 5181-1519 CAN cable, Agilent module to module, 1 m 5988-8453EN Capillary/fitting starter kit brochure 9222-0519...

  • Page 47: Optimizing The Stack Configuration

    Installing the Pump Optimizing the Stack Configuration Optimizing the Stack Configuration If your module is part of a complete Agilent 1260 Infinity Liquid Chromatograph, you can ensure optimum performance by installing the following configurations. These configurations optimize the system flow path, ensuring minimum delay volume.
  • Page 48 Installing the Pump Optimizing the Stack Configuration Figure 10 Recommended Stack Configuration (Front View) Agilent 1260 IsoPump/QuatPump User Manual...
  • Page 49 Installing the Pump Optimizing the Stack Configuration Figure 11 Recommended Stack Configuration (Rear View) Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 50: Installing The Pump

    Installing the Pump Installing the Pump Installing the Pump Parts required Description Pump Data System G4208A Instant Pilot Power cord For other cables see text below and “Cable Overview” on page 166. Preparations • Locate bench space. • Provide power connections. •...
  • Page 51 Installing the Pump Installing the Pump 2 Ensure the power switch on the front of the module is OFF (switch stands out). Figure 12 Front of Pump 3 Connect the power cable to the power connector at the rear of the module. Agilent 1260 IsoPump/QuatPump User Manual...
  • Page 52 Installing the Pump Installing the Pump 4 Connect the required interface cables to the quaternary pump, see “Connecting Modules and Control Software” on page 53. 5 Connect all capillaries, solvent tubes and waste tubing (see “Flow Connections of the Pump” on page 56).

  • Page 53: Connecting Modules And Control Software

    Connecting Modules and Control Software Use of unsupplied cables WA R N I N G Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

  • Page 54: Connecting A Vacuum Degasser

    Installing the Pump Connecting Modules and Control Software Connecting a Vacuum Degasser The quaternary pump has a built-in degasser. For the isocratic pump, an external degasser N O T E can be used and a pump upgrade to the quaterary pump including a built-in degasser is possible.

  • Page 55: Connecting Control Software And/Or G4208 A Instant Pilot

    Installing the Pump Connecting Modules and Control Software Connecting Control Software and/or G4208 A Instant Pilot With the introduction of the Agilent 1260 Infinity, all GPIB interfaces have been removed. N O T E The preferred communication is LAN. Usually the detector is producing the most data in the stack, followed by the pump, and it is N O T E therefore highly recommended to use either of these modules for the LAN connection.

  • Page 56: Flow Connections Of The Pump

    Installing the Pump Flow Connections of the Pump Flow Connections of the Pump Parts required Description Other modules Parts from accessory kit wrenches 1/4 - 5/16 inch for capillary connections Preparations Pump is installed in the LC system Toxic, flammable and hazardous solvents, samples and reagents WA R N I N G The handling of solvents, samples and reagents can hold health and safety risks.
  • Page 57 6 Using a piece of sanding paper connect the waste tubing to the purge valve and place it into your waste system. 7 If the pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack, connect the corrugated waste tube to the waste outlet of the pump leak handling system.
  • Page 58 Installing the Pump Flow Connections of the Pump 9 Prime your system before first use (see “Initial Priming” on page 59). Figure 14 Flow Connections of the Quaternary Pump Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 59: Priming The System

    Installing the Pump Priming the System Priming the System Initial Priming When Before a new degasser or new solvent tubing can be used, it is necessary to prime the system. Isopropanol (IPA) is recommended as priming solvent due to its miscibility with nearly all HLPC solvents and its excellent wetting properties.
  • Page 60 Installing the Pump Priming the System When priming the vacuum degasser with a syringe, the solvent is drawn through the N O T E degasser tubes very quickly. The solvent at the degasser outlet will therefore not be fully degassed. Pump for approximately 10 minutes at your desired flow rate before starting an analysis.

  • Page 61: Regular Priming

    Installing the Pump Priming the System Regular Priming When When the pumping system has been turned off for a certain time (for example, overnight) air will rediffuse into the solvent channel between the vacuum degasser and the pump. Solvents containing volatile ingredients will slightly lose these if left in the degasser without flow for a prolonged period of time.

  • Page 62: Changing Solvents

    Installing the Pump Priming the System Changing Solvents When When the solvent of a channel is to be replaced by another solvent that is not compatible (solvents are immiscible or one solvent contains a buffer) it is necessary to follow the procedure below to prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system.
  • Page 63 Installing the Pump Priming the System Table 18 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of When switching between Isopropanol the system reverse phase and normal Miscible with almost all phase (both times) solvents After an installation...
  • Page 64 Installing the Pump Priming the System Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 65: Using The Pump

    Using the Pump Hints for Successful Use of the Isocratic Pump Prevent Blocking of Solvent Filters Algae Growth in HPLC Systems How to Prevent and/or Reduce the Algae Problem This chapter explains the operational parameters of the module. Agilent Technologies...

  • Page 66: Hints For Successful Use Of The Isocratic Pump

    • Always place solvent cabinet with the solvent bottle(s) on top of the pump (or at a higher level). • When using salt solutions and organic solvents in the Agilent 1260 Infinity Quaternary Pump it is recommended to connect the salt solution to one of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve ports.
  • Page 67 • Always place the solvent cabinet with the solvent bottles on top of the quaternary pump (or at a higher level). • When using salt solutions and organic solvents in the Agilent 1260 Infinity Quaternary Pump VL it is recommended to connect the salt solution to one of the bottom gradient valve ports and the organic solvent to one of the upper gradient valve ports.

  • Page 68: Prevent Blocking Of Solvent Filters

    Using the Pump Prevent Blocking of Solvent Filters Prevent Blocking of Solvent Filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module. This is especially true for aqueous solvents or phosphate buffers (pH 4 to 7). The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the module.

  • Page 69: Algae Growth In Hplc Systems

    Agilent 1260 Infinity where helium is not used for degassing (most algae need oxygen and light for growth). The presence of algae in the Agilent 1260 Infinity can cause the following to occur: • PTFE frits, PTFE frit (pack of 5) (p/n 01018-22707), (purge valve assembly) and column filter blockage causing increased system pressure.
  • Page 70 Using the Pump Algae Growth in HPLC Systems frits to block over short-term usage. Please refer to the section “Exchanging the Purge Valve Frit or the Purge Valve” on page 126 in this manual. • Short lifetime of solvent filters (bottle head assembly). A blocked solvent filter in the bottle, especially when only partly blocked, is more difficult to identify and may show up as gradient performance problems, intermittent pressure fluctuations etc.

  • Page 71: Optimiziming Performance

    Operational Hints for the Multi Channel Gradient Valve (MCGV) When to use the Seal Wash Option Choosing the Right Pump Seals Optimize the Compressibility Compensation Setting This chapter gives hints on how to optimize the performance or use additional devices. Agilent Technologies...

  • Page 72: When To Use A Vacuum Degasser

    • pump was turned off for a length of time (for example, during night) and volatile solvent mixtures are used, or • solvents have been changed. For more information see the Agilent 1260 Infinity Standard Degasser User Manual (p/n G1322-90012). Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 73: Operational Hints For The Multi Channel Gradient Valve (Mcgv)

    Optimiziming Performance Operational Hints for the Multi Channel Gradient Valve (MCGV) Operational Hints for the Multi Channel Gradient Valve (MCGV) In a mixture of salt solutions and organic solvent the salt solution might be well dissolved in the organic solvent without showing precipitations. However in the mixing point of the gradient valve, at the boundary between the two solvents, micro precipitation is possible.

  • Page 74: When To Use The Seal Wash Option

    Optimiziming Performance When to use the Seal Wash Option When to use the Seal Wash Option Highly concentrated buffer solutions will reduce the lifetime of the seals and pistons in your pump. The seal wash option allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent.
  • Page 75 Optimiziming Performance When to use the Seal Wash Option When to Use the Seal Wash Option Highly concentrated buffer solutions will reduce the lifetime of the seals and pistons in your pump. The seal wash option allows to maintain the seal lifetime by flushing the back side of the seal with a wash solvent.

  • Page 76: Choosing The Right Pump Seals

    Optimiziming Performance Choosing the Right Pump Seals Choosing the Right Pump Seals The standard seal for the pump can be used for most applications. However applications that use normal phase solvents (for example, hexane) are not suited for the standard seal and require a different seal when used for a longer time in the pump.

  • Page 77: Optimize The Compressibility Compensation Setting

    Optimiziming Performance Optimize the Compressibility Compensation Setting Optimize the Compressibility Compensation Setting The compressibility compensation default setting is 100 × 10 /bar for the pump. This setting represents an average value. Under normal conditions the default setting reduces the pressure pulsation to values (below 1% of system pressure) that will be sufficient for most applications and for all gradient analyses.
  • Page 78 Optimiziming Performance Optimize the Compressibility Compensation Setting Table 19 Solvent Compressibility Solvent (pure) Compressibility (10-6/bar) Acetone Acetonitrile Benzene Carbon tetrachloride Chloroform Cyclohexane Ethanol Ethyl acetate Heptane Hexane Isobutanol Isopropanol Methanol 1-Propanol Toluene Water Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 79: Troubleshooting And Diagnostics

    Overview of the Module’s Indicators and Test Functions Status Indicators Power Supply Indicator Module Status Indicator User Interfaces Agilent Lab Advisor Software This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces. Agilent Technologies...

  • Page 80: Overview Of The Module's Indicators And Test Functions

    Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Overview of the Module’s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state (prerun, run, and error states) of the module. The status indicators provide a quick visual check of the operation of the module.
  • Page 81 Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Leak Rate Test The Leak Rate Test is a diagnostic test designed to determine the pressure tightness of the pump. When a problem with the pump is suspected, use this test to help troubleshoot the pump and its pumping performance.

  • Page 82: Status Indicators

    Troubleshooting and Diagnostics Status Indicators Status Indicators Two status indicators are located on the front of the module. The lower left indicates the power supply status, the upper right indicates the module status. Figure 15 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch.

  • Page 83: Module Status Indicator

    Troubleshooting and Diagnostics Status Indicators Module Status Indicator The module status indicator indicates one of six possible module conditions: • When the status indicator is OFF (and power switch light is on), the module is in a prerun condition, and is ready to begin an analysis. •...

  • Page 84: User Interfaces

    Troubleshooting and Diagnostics User Interfaces User Interfaces Depending on the user interface, the available tests vary. Some descriptions are only available in the service manual. Table 20 Test functions available vs. user interface Test Instant Pilot G4208A Agilent Lab Advisor System Pressure Test Leak Rate Test Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 85: Agilent Lab Advisor Software

    Troubleshooting and Diagnostics Agilent Lab Advisor Software Agilent Lab Advisor Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system. Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet.
  • Page 86 Troubleshooting and Diagnostics Agilent Lab Advisor Software Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 87: Error Information

    Restart Without Cover Solvent Zero Counter Pressure Above Upper Limit Pressure Below Lower Limit Pressure Signal Missing Missing Pressure Reading Pump Configuration Valve Fuse Inlet-Valve Fuse Valve Failed (MCGV/SSV) Motor-Drive Power Encoder Missing Inlet-Valve Missing Temperature Out of Range Agilent Technologies...
  • Page 88 Error Information Agilent Lab Advisor Software Temperature Limit Exceeded Servo Restart Failed Pump Head Missing Index Limit Index Adjustment Index Missing Stroke Length Initialization Failed Wait Timeout Degasser: cannot read signal Degasser: limit not reached This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.

  • Page 89: What Are Error Messages

    Error Information What Are Error Messages What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary).

  • Page 90: General Error Messages

    Error Information General Error Messages General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. Timeout The timeout threshold was exceeded. Probable cause Suggested actions Check the logbook for the occurrence and The analysis was completed successfully, source of a not-ready condition.

  • Page 91: Remote Timeout

    Error Information General Error Messages Probable cause Suggested actions Check external instruments for a shut-down Shut-down in an external instrument with a condition. remote connection to the system. Check the vacuum degasser for an error The degasser failed to generate sufficient condition.

  • Page 92: Synchronization Lost

    Error Information General Error Messages Synchronization Lost During an analysis, the internal synchronization or communication between one or more of the modules in the system has failed. The system processors continually monitor the system configuration. If one or more of the modules is no longer recognized as being connected to the system, the error message is generated.

  • Page 93: Leak Sensor Open

    Error Information General Error Messages Leak Sensor Open The leak sensor in the module has failed (open circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak-sensor current to change within defined limits.

  • Page 94: Compensation Sensor Open

    Error Information General Error Messages Compensation Sensor Open The ambient-compensation sensor (NTC) on the main board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the main board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes.

  • Page 95: Fan Failed

    Error Information General Error Messages Fan Failed The cooling fan in the module has failed. The hall sensor on the fan shaft is used by the main board to monitor the fan speed. If the fan speed falls below a certain limit for a certain length of time, the error message is generated.

  • Page 96: Module Error Messages

    Error Information Module Error Messages Module Error Messages These errors are pump specific. Restart Without Cover The module was restarted with the top cover and foam open. The sensor on the main board detects when the top foam is in place. If the module is restarted with the foam removed, the module switches off within 30 s, and the error message is generated.

  • Page 97: Pressure Above Upper Limit

    Error Information Module Error Messages Pressure Above Upper Limit The system pressure has exceeded the upper pressure limit. Probable cause Suggested actions Ensure the upper pressure limit is set to a value Upper pressure limit set too low. suitable for the analysis. Check for blockage in the flowpath.

  • Page 98: Pressure Signal Missing

    Error Information Module Error Messages Probable cause Suggested actions Please contact your Agilent service Defective damper. representative. Please contact your Agilent service Defective main board. representative. Pressure Signal Missing The pressure signal of the damper is missing. The pressure signal of the damper must be within a specific voltage range. If the pressure signal is missing, the processor detects a voltage of approximately -120mV across the damper connector.

  • Page 99: Pump Configuration

    Error Information Module Error Messages Pump Configuration At switch-on, the quaternary pump has recognized a new pump configuration. The quaternary pump is assigned its configuration at the factory. If the gradient valve is disconnected, and the quaternary pump is rebooted, the error message is generated.

  • Page 100: Inlet-Valve Fuse

    Error Information Module Error Messages Inlet-Valve Fuse The active-inlet valve in the module has drawn excessive current causing the inlet-valve electronic fuse to open. Probable cause Suggested actions Restart the module. If the error message Defective active inlet valve. appears again, exchange the active inlet valve. Please contact your Agilent service Defective connection cable (front panel to representative.

  • Page 101: Motor-Drive Power

    Error Information Module Error Messages Probable cause Suggested actions Please contact your Agilent service Connection cable (inside instrument) representative. defective. Exchange the gradient valve. Gradient valve defective. Motor-Drive Power The current drawn by the pump motor exceeded the maximum limit. Blockages in the flow path are usually detected by the pressure sensor in the damper, which result in the pump switching off when the upper pressure limit is exceeded.

  • Page 102: Encoder Missing

    Error Information Module Error Messages Encoder Missing The optical encoder on the pump motor in the module is missing or defective. The processor checks the presence of the pump encoder connector every 2 seconds. If the connector is not detected by the processor, the error message is generated.

  • Page 103: Temperature Out Of Range

    Error Information Module Error Messages Temperature Out of Range The temperature sensor readings in the motor-drive circuit are out of range. The values supplied to the ADC by the hybrid sensors must be between 0.5 V and 4.3 V. If the values are outside this range, the error message is generated. Probable cause Suggested actions Please contact your Agilent service...

  • Page 104: Servo Restart Failed

    Error Information Module Error Messages Servo Restart Failed The pump motor in the module was unable to move into the correct position for restarting. When the module is switched on, the first step is to switch on the C phase of the variable reluctance motor.

  • Page 105: Index Limit

    Error Information Module Error Messages Index Limit The time required by the piston to reach the encoder index position was too short (pump). During initialization, the first piston is moved to the mechanical stop. After reaching the mechanical stop, the piston reverses direction until the encoder index position is reached.

  • Page 106: Index Missing

    Error Information Module Error Messages Index Missing The encoder index position in the module was not found during initialization. During initialization, the first piston is moved to the mechanical stop. After reaching the mechanical stop, the piston reverses direction until the encoder index position is reached.

  • Page 107: Initialization Failed

    Error Information Module Error Messages Initialization Failed The module failed to initialize successfully within the maximum time window. A maximum time is assigned for the complete pump-initialization cycle. If the time is exceeded before initialization is complete, the error message is generated.

  • Page 108: Degasser: Cannot Read Signal

    Error Information Module Error Messages Probable cause Suggested actions Ensure that purge valve is closed. Purge valve open. Ensure pump components are seated correctly. Leak at fittings, purge valve, active inlet If there are still signs of a leak, exchange the valve, outlet valve or piston seals.

  • Page 109: Degasser: Limit Not Reached

    Error Information Module Error Messages Degasser: limit not reached This error is thrown, if the degasser does not become ready after 8 min, i.e. is higher than 180 mbar. Probable cause Suggested actions Please contact your Agilent service Liquid in degasser tubing. representative.
  • Page 110 Error Information Module Error Messages Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 111: Test Functions And Calibration

    Agilent 1260 IsoPump/QuatPump User Manual Test Functions and Calibration Introduction System Pressure Test System Pressure Test failed Leak Rate Test Leak Rate Test Description This chapter describes the tests for the module. Agilent Technologies...

  • Page 112: Introduction

    Test Functions and Calibration Introduction Introduction All tests are described based on the Agilent Lab Advisor Software B.01.03. Other user interfaces may not provide any test or just a few. For details on the use of the interface refer to the interface documentation. Table 21 Interfaces and available test functions Interface...

  • Page 113: System Pressure Test

    Test Functions and Calibration System Pressure Test System Pressure Test Description The System Pressure Test is a quick, built-in test designed to demonstrate the pressure-tightness of the system. The test is required, if problems with small leaks are suspected, or after maintenance of flow-path components (e.g., pump seals, injection seal) to prove pressure tightness up to 600 bar.

  • Page 114: Leak Rate Test

    Test Functions and Calibration Leak Rate Test Leak Rate Test Leak Rate Test Description The Leak Rate Test is a built-in troubleshooting test designed to demonstrate the leak-tightness of the pump. For running the Leak Rate Test and evaluating test results, please refer to the online help of LabAdvisor.

  • Page 115: Maintenance

    Maintenance of a Pump Head with Seal Wash Option Reinstalling the Pump Head Assembly Exchanging the Multi-Channel Gradient Valve (MCGV) Exchanging the Optional Interface Board Exchanging the Active Inlet Valve (AIV) or its Cartridge Replacing the Module’s Firmware This chapter describes the maintenance of the module. Agilent Technologies...

  • Page 116: Introduction To Maintenance And Repair

    Maintenance Introduction to Maintenance and Repair Introduction to Maintenance and Repair The module is designed for easy repair. The most frequent repairs such as piston seal change and purge valve frit change can be done from the front of the module with the module in place in the system stack. These repairs are described in “Overview of Maintenance”...

  • Page 117: Warnings And Cautions

    Maintenance Warnings and Cautions Warnings and Cautions Toxic, flammable and hazardous solvents, samples and reagents WA R N I N G The handling of solvents, samples and reagents can hold health and safety risks. ➔ When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice.
  • Page 118 Maintenance Warnings and Cautions Safety standards for external equipment C A U T I O N ➔ If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment.

  • Page 119: Overview Of Maintenance

    Maintenance Overview of Maintenance Overview of Maintenance The following pages describe maintenance (simple repairs) of the pump that can be carried out without opening the main cover. Table 22 Simple Repair Procedures Procedure Typical Frequency Notes “Checking and Cleaning the Solvent If solvent filter is blocked Gradient performance problems, intermittent Filter”...

  • Page 120: Cleaning The Module

    Maintenance Cleaning the Module Cleaning the Module The module case should be kept clean. Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent. Do not use an excessively damp cloth as liquid may drip into the module. Liquid dripping into the electronic compartment of your module.

  • Page 121: Checking And Cleaning The Solvent Filter

    Maintenance Checking and Cleaning the Solvent Filter Checking and Cleaning the Solvent Filter Small particles can permanently block the capillaries and valves of the module. C A U T I O N Damage of the module. ➔ Always filter solvents. ➔...

  • Page 122: Cleaning The Solvent Filter

    Maintenance Checking and Cleaning the Solvent Filter Cleaning the Solvent Filter When If solvent filter is blocked Parts required Description Concentrated nitric acid (35 %) LC grade water Beaker Preparations Remove solvent inlet tube from the adapter at the inlet valve. 1 Remove the blocked solvent filter from the bottle-head assembly and place it in a beaker with concentrated nitric acid (35%) for one hour.

  • Page 123: Exchanging The Passive Inlet Valve (Piv)

    Maintenance Exchanging the Passive Inlet Valve (PIV) Exchanging the Passive Inlet Valve (PIV) When If internally leaking (backflow) Tools required • Wrench 14 mm • Pair of Tweezers Parts required Description G1312-60066 Passive inlet valve 1 Remove the front cover. 2 Disconnect the solvent inlet tube from the inlet valve (be aware that solvent may leak out of the tube due to hydrostatic flow).

  • Page 124: Exchanging The Outlet Valve

    Maintenance Exchanging the Outlet Valve Exchanging the Outlet Valve When If internally leaking Tools required Wrench 1/4 inch Wrench 14 mm Parts required Description G1312-60067 Outlet valve, complete Preparations • Switch off pump at the main power switch • Remove the front cover Before exchanging the outlet valve you can try to clean it in a sonic bath for 5 –...
  • Page 125 Maintenance Exchanging the Outlet Valve Reinstall the outlet valve and tighten the valve. Reconnect the valve capillary. Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 126: Exchanging The Purge Valve Frit Or The Purge Valve

    Maintenance Exchanging the Purge Valve Frit or the Purge Valve Exchanging the Purge Valve Frit or the Purge Valve When • Frit – when piston seals are exchanged or when contaminated or blocked (pressure drop of > 10 bar across the frit at a flow rate of 5 mL/min of H 0 with purge valve opened) •...
  • Page 127 Maintenance Exchanging the Purge Valve Frit or the Purge Valve Place a new frit into the purge valve with the orientation Insert the purge valve into the pump head and locate the of the frit as shown above. pump outlet capillary and the waste tube. Reinstall the cap with the gold seal.

  • Page 128: Removing The Pump Head Assembly

    Maintenance Removing the Pump Head Assembly Removing the Pump Head Assembly When • Exchanging the seals • Exchanging the pistons • Exchanging seals of the seal wash option Tools required • Wrench 1/4 inch • 4-mm hexagonal key Preparations • Switch off pump at the main power switch and unplug the power cable •...
  • Page 129 Maintenance Removing the Pump Head Assembly Next Steps: Using a 4 mm hexagonal key, stepwise loosen the two pump head screws and remove the pump head from the pump drive. Disconnect the capillary from the outlet valve. Remove the waste tubing and disconnect the solvent tubing from the inlet valve.

  • Page 130: Maintenance Of A Pump Head Without Seal Wash Option

    Maintenance Maintenance of a Pump Head Without Seal Wash Option Maintenance of a Pump Head Without Seal Wash Option When In case of maintenance or pump head internal leaks. Tools required • Wrench 1/4 inch • 4-mm hexagonal key Parts required Description 01018-23702 Insert tool...
  • Page 131 Maintenance Maintenance of a Pump Head Without Seal Wash Option Place the pump head on a flat surface. Loosen the lock Remove the support rings from the piston housing and lift screw (two revolutions) and while holding the lower half the housing away from the pistons.
  • Page 132 Maintenance Maintenance of a Pump Head Without Seal Wash Option Check the piston surface and remove any deposits or Using the insert tool carefully remove the seal from the layers. Cleaning can be done with alcohol or tooth paste. pump head. Remove wear retainers, if still present. Replace piston if scratched.
  • Page 133 Maintenance Maintenance of a Pump Head Without Seal Wash Option Next Steps: If a standard seal has been installed, run the seal wear-in procedure, see “Seal Wear-in Procedure” on page 134. For the normal phase seal, the purge valve frit should be replaced, see “Exchanging the Purge Valve Frit or the Purge Valve”...

  • Page 134: Seal Wear-In Procedure

    Maintenance Seal Wear-in Procedure Seal Wear-in Procedure Parts required Description 0100-1847 Adapter AIV to solvent inlet tubes 5022-2159 Restriction capillary Seal damage C A U T I O N ➔ This procedure is required for Standard seals (pack of 2) (p/n 5063-6589), but it will damage the PE seals (pack of 2) (p/n 0905-1420).

  • Page 135: Maintenance Of A Pump Head With Seal Wash Option

    Maintenance Maintenance of a Pump Head with Seal Wash Option Maintenance of a Pump Head with Seal Wash Option When When maintaining seal wash option Tools required • 4-mm hexagonal key Parts required Description 01018-23702 Insert tool 0905-1175 Wash seal 5062-2484 Gasket, seal wash (pack of 6) Preparations...
  • Page 136 Maintenance Maintenance of a Pump Head with Seal Wash Option Remove the seal holder and the seal wash support rings Using the blade of a flat-blade screwdriver remove the from the piston housing. Remove the seal holder from the seal wash gasket and the secondary seal from the support ring assembly.
  • Page 137 Maintenance Maintenance of a Pump Head with Seal Wash Option Place the support rings on the piston housing (pistons not Insert the pistons and carefully press them into the seal. installed) and snap the pump head and piston housing together. Tighten the lock screw.

  • Page 138: Reinstalling The Pump Head Assembly

    Maintenance Reinstalling the Pump Head Assembly Reinstalling the Pump Head Assembly When When reassembling the pump Tools required • 4-mm hexagonal key Parts required Description 79846-65501 Pump head grease Apply a small amount of grease on the back of the Slide the pump head assembly onto the pump drive.
  • Page 139 Maintenance Reinstalling the Pump Head Assembly Using a 4 mm hexagonal key tighten the pump head Reconnect all capillaries, tubes and (if installed) the screws stepwise with increasing torque. active inlet valve cable to its connector. Reinstall the front cover. Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 140: Exchanging The Multi-Channel Gradient Valve (Mcgv)

    When using buffers in combination with organic solvents in the Agilent 1260 Infinity Quaternary Pump it is recommended to connect the aequous solutions/buffers to one of the bottom ports and the organic solvent to one of the upper gradient valve ports.
  • Page 141 Maintenance Exchanging the Multi-Channel Gradient Valve (MCGV) Disconnect the connecting tube, waste tube and the Press the lower sides of the cover to unclip it. Remove the solvent tubes from the MCGV, unclip them from the tube cover. clips and place them into the solvent cabinet to avoid flow by hydrostatic pressure.
  • Page 142 Maintenance Exchanging the Multi-Channel Gradient Valve (MCGV) Install the MCGV cover. Reconnect the waste funnel with Reconnect the tube from the inlet valve to the middle the waste tube holder in the top cover. Insert waste tube position of the MCGV. Connect solvent tubes for channels in the holder in the leak pan and clip tube to the MCGV A-D from the MCGV to the degasser outlets.

  • Page 143: Exchanging The Optional Interface Board

    Maintenance Exchanging the Optional Interface Board Exchanging the Optional Interface Board When Board defective Parts required Description G1351-68701 Interface board (BCD) with external contacts and BCD outputs Electronic boards are static sensitive and should be handled with care so as not to C A U T I O N damage them.
  • Page 144 Maintenance Exchanging the Optional Interface Board 6 Reconnect the pump to line power. Figure 17 Exchanging the Interface Board Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 145: Exchanging The Active Inlet Valve (Aiv) Or Its Cartridge

    This is a configuration change which is not covered by the specifications for this module. By default, 1260 Infinity pumps do not have an active inlet valve. The first time an AIV shall N O T E be installed, a connector and cable to the main board must be installed by the service and a different connection tube is needed.
  • Page 146 Maintenance Exchanging the Active Inlet Valve (AIV) or its Cartridge 5 Using a 14 mm wrench loosen the active inlet valve and remove the valve from the pump head. Figure 18 Active Inlet Valve Assembly 6 Using a pair of tweezers remove the valve cartridge from the actuator assembly.

  • Page 147: Replacing The Module's Firmware

    Maintenance Replacing the Module’s Firmware Replacing the Module’s Firmware When The installation of newer firmware might be necessary • if a newer version solves problems of older versions or • to keep all systems on the same (validated) revision. The installation of older firmware might be necessary •...
  • Page 148 Maintenance Replacing the Module’s Firmware Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 149: Parts For Maintenance

    Pump Head Assembly with Seal Wash Option (600 bar) Outlet Valve Purge Valve Assembly Active Inlet Valve Assembly Accessory Kit G1311-68755 Seal Wash Option Kit Solvent Cabinet Bottle Head Assembly Hydraulic Path This chapter provides information on parts for maintenance. Agilent Technologies...

  • Page 150: Pump Head Assembly Without Seal Wash

    Parts for Maintenance Pump Head Assembly Without Seal Wash Pump Head Assembly Without Seal Wash Item p/n Description G1312-60064 Pump Head without Seal Wash 5067-4695 Sapphire piston (default) G1312-60062 Piston housing (incl. spring) G4220-63015 Support Ring without Seal Wash 0905-1503 Piston seal PTFE, carbon filled, black (pack of 2), default 0905-1719 Pump Seal PE, yellow (pack of 2)
  • Page 151 Parts for Maintenance Pump Head Assembly Without Seal Wash Figure 19 Pump Head Assembly without seal wash option Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 152: Pump Head Assembly With Seal Wash Option (600 Bar)

    Parts for Maintenance Pump Head Assembly with Seal Wash Option (600 bar) Pump Head Assembly with Seal Wash Option (600 bar) Item p/n Description G1312-60065 Pump Head with Seal Wash 5067-4695 Sapphire piston (default) G1312-60062 Piston housing (incl. spring) G4220-63010 Support Ring (Seal Wash) 0905-1175 Wash seal PTFE, carbon filled...
  • Page 153 Parts for Maintenance Pump Head Assembly with Seal Wash Option (600 bar) Item p/n Description 0515-2118 Screw M5, 60 mm long G1311-60161 Seal wash pump assembly Figure 20 Pump Head with Seal Wash Option Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 154: Outlet Valve

    Parts for Maintenance Outlet Valve Outlet Valve Description G1312-60067 Outlet valve, complete Figure 21 Outlet Valve Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 155: Purge Valve Assembly

    Parts for Maintenance Purge Valve Assembly Purge Valve Assembly Item p/n Description G1312-60061 Purge valve assembly 01018-22707 PTFE frit (pack of 5) 5067-4728 Seal cap Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 156: Active Inlet Valve Assembly

    Parts for Maintenance Active Inlet Valve Assembly Active Inlet Valve Assembly Item p/n Description G1312-60025 Active inlet valve without cartridge G1312-60020 Cartridge for active inlet valve 600bar Figure 22 Active Inlet Valve Assembly Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 157: Accessory Kit G1311-68755

    Parts for Maintenance Accessory Kit G1311-68755 Accessory Kit G1311-68755 Accessory Kit G1311-68755 Description 5062-2461 Waste tube, 5 m (reorder pack) 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) 5181-1519 CAN cable, Agilent module to module, 1 m 5988-8453EN Capillary/fitting starter kit brochure 9222-0519...

  • Page 158: Seal Wash Option Kit

    Parts for Maintenance Seal Wash Option Kit Seal Wash Option Kit Description G1311-60161 Seal wash pump assembly 5042-8507 Seal wash pump cartridge (silicone tubing) 5062-2465 Support ring, seal wash 0905-1175 Secondary seal (pre-installed in support rings) 5062-2484 Gasket, seal wash (pack of 6) 5001-3743 Seal holder 0890-1764...

  • Page 159: Solvent Cabinet

    Parts for Maintenance Solvent Cabinet Solvent Cabinet Item p/n Description 5065-9981 Solvent cabinet, including all plastic parts 5043-0207 Name plate 1260 5042-8567 Leak pan 9301-1420 Solvent bottle, transparent 9301-1450 Solvent bottle, amber G1311-60003 Bottle-head assembly Agilent 1260 IsoPump/QuatPump User Manual...
  • Page 160 Parts for Maintenance Solvent Cabinet Figure 23 Solvent Cabinet Parts Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 161: Bottle Head Assembly

    Parts for Maintenance Bottle Head Assembly Bottle Head Assembly Item p/n Description G1311-60003 Bottle-head assembly 5063-6598 Ferrules with lock ring (10x) 5063-6599 Tube screw (10x) Wire marker 5062-2483 Solvent tubing, 5 m 5062-8517 Inlet filter adapter (pack of 4) 5041-2168 Solvent inlet filter, 20 µm Figure 24 Bottle-Head Assembly Parts...

  • Page 162: Hydraulic Path

    Parts for Maintenance Hydraulic Path Hydraulic Path Item p/n Description G1311-67301 Capillary, piston 1 to damper G1311-60003 Bottle-head assembly G1311-67300 Capillary, damper to piston 2 G1312-67305 Outlet capillary, pump to injector device G1329-87300 Outlet capillary, pump to thermostattable autosampler 5062-2461 Waste tube, 5 m (reorder pack) 0100-1847 Adapter AIV to solvent inlet tubes...
  • Page 163 Parts for Maintenance Hydraulic Path Hydraulic Path Item p/n Description G1312-67305 Outlet capillary, pump to injector device G1329-87300 Outlet capillary, pump to thermostattable autosampler G1311-60003 Bottle-head assembly G1322-67300 Kit of 4 solvent tubes for connection degasser to MCGV (Quaternary Pump) including labels G1311-81600 Capillary, piston 1 to damper G1311-81601...
  • Page 164 Parts for Maintenance Hydraulic Path Figure 26 Hydraulic Flow Path of the Quaternary Pump Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 165: Identifying Cables

    Identifying Cables Cable Overview Analog Cables Remote Cables BCD Cables CAN/LAN Cables External Contact Cable Agilent Module to PC Agilent 1200 Module to Printer This chapter provides information on cables used with the Agilent 1200 Infinity Series modules. Agilent Technologies...

  • Page 166: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent module to 3394/6 integrators...
  • Page 167 Identifying Cables Cable Overview CAN cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) External Contact Cable...

  • Page 168: Analog Cables

    Identifying Cables Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 3394/6 Integrators p/n 35900-60750 Pin 3394/6 Pin Agilent Signal Name...
  • Page 169 Identifying Cables Analog Cables Agilent Module to BNC Connector p/n 8120-1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin 3394/6 Pin Agilent Signal Name module Not connected Black Analog -...

  • Page 170: Remote Cables

    Identifying Cables Remote Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent Module to 3396A Integrators...
  • Page 171 Identifying Cables Remote Cables Agilent Module to 3396 Series III / 3395B Integrators p/n 03396-61010 Pin 33XX Pin Agilent Signal Name Active module (TTL) 1 - White Digital ground 2 - Brown Prepare run 3 - Gray Start 4 - Blue Shut down 5 - Pink Not connected...
  • Page 172 Identifying Cables Remote Cables Agilent Module to General Purpose p/n 01046-60201 Pin Universal Pin Agilent Signal Name Active module (TTL) 1 - White Digital ground 2 - Brown Prepare run 3 - Gray Start 4 - Blue Shut down 5 - Pink Not connected 6 - Yellow Power on...

  • Page 173: Bcd Cables

    Identifying Cables BCD Cables BCD Cables One end of these cables provides a 15-pin BCD connector to be connected to the Agilent modules. The other end depends on the instrument to be connected to Agilent Module to General Purpose p/n G1351-81600 Wire Color Pin Agilent Signal Name...
  • Page 174 Identifying Cables BCD Cables Agilent Module to 3396 Integrators p/n 03396-60560 Pin 3396 Pin Agilent Signal Name BCD Digit module BCD 5 BCD 7 BCD 6 BCD 4 BCD0 BCD 3 BCD 2 BCD 1 Digital ground + 5 V Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 175: Can/Lan Cables

    Identifying Cables CAN/LAN Cables CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. CAN Cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203...

  • Page 176: External Contact Cable

    Identifying Cables External Contact Cable External Contact Cable One end of this cable provides a 15-pin plug to be connected to Agilent modules interface board. The other end is for general purpose. Agilent Module Interface Board to general purposes p/n G1103-61611 Color Pin Agilent Signal Name...

  • Page 177: Agilent Module To Pc

    Identifying Cables Agilent Module to PC Agilent Module to PC Description G1530-60600 RS-232 cable, 2 m RS232-61600 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It's also called "Null Modem Cable"...

  • Page 178: Agilent 1200 Module To Printer

    Identifying Cables Agilent 1200 Module to Printer Agilent 1200 Module to Printer Description 5181-1529 Cable Printer Serial & Parallel, is a SUB-D 9 pin female vs. Centronics connector on the other end (NOT FOR FW UPDATE). For use with G1323 Control Module. Agilent 1260 IsoPump/QuatPump User Manual...

  • Page 179: Appendix

    Agilent 1260 IsoPump/QuatPump User Manual Appendix General Safety Information The Waste Electrical and Electronic Equipment Directive Batteries Information Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet This chapter provides addition information on safety, legal and web. Agilent Technologies...

  • Page 180: General Safety Information

    Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.
  • Page 181 Appendix General Safety Information Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuse holders must be avoided. Some adjustments described in the manual, are made with power supplied to the instrument, and protective covers removed.
  • Page 182 Appendix General Safety Information Safety Symbols Table 23 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage.

  • Page 183: The Waste Electrical And Electronic Equipment Directive

    Appendix The Waste Electrical and Electronic Equipment Directive The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC), adopted by EU Commission on 13 February 2003, is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005.

  • Page 184: Batteries Information

    Appendix Batteries Information Batteries Information Lithium batteries may not be disposed-off into the domestic waste. Transportation of WA R N I N G discharged Lithium batteries through carriers regulated by IATA/ICAO, ADR, RID, IMDG is not allowed. Danger of explosion if battery is incorrectly replaced. ➔...

  • Page 185: Radio Interference

    Appendix Radio Interference Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or...

  • Page 186: Sound Emission

    Appendix Sound Emission Sound Emission Manufacturer’s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB. •...

  • Page 187: Solvent Information

    Appendix Solvent Information Solvent Information Observe the following recommendations on the use of solvents. • Brown glass ware can avoid growth of algae. • Small particles can permanently block capillaries and valves. Therefore always filter solvents through 0.4 µm filters. •...

  • Page 188: Agilent Technologies On Internet

    Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select Products/Chemical Analysis It will provide also the latest firmware of the modules for download.
  • Page 189 Index Index AUX output cleaning Communication settings solvent zero counter RS-232C compensation sensor open ball-screw drive compensation sensor short battery configuration composition precision safety information one stack composition range compressibility compensation 15, 40, cable bench space 8-bit configuration switch condensation blockage on-board LAN configuration...
  • Page 190 Index pump error multi channel gradient valve (MCGV) error messages index limit outlet valve 119, compensation sensor open index adjustment passive inlet valve compensation sensor short index missing pistons encoder missing initialization failed pump seals ignition without cover initialization purge valve frit 119, index adjustment purge valve...
  • Page 191 Index missing pressure reading power consideration missing parts power consumption safety class I motor-drive power power cords safety information multi channel gradient valve power switch lithium batteries (MCGV) pressure above upper limit safety pressure below lower limit general information pressure sensor readings standards non-operating altitude pressure pulsation...
  • Page 192 Index special settings variable reluctance motor boot-resident variable stroke volume forced cold start voltage range specification physical status indicator wait timeout stroke volume waste stroke length electrical and electronic stroke volume equipment synchronization lost wear-in system setup and installation procedure optimizing stack configuration WEEE directive weight...
  • Page 193 Index Agilent 1260 IsoPump/QuatPump User Manual...
  • Page 194 In This Book This manual contains technical information about the Agilent 1260 Infinity Isocratic Pump (G1310B) and Quaternary Pump (G1311B). The manual describes the following: • introduction, • site requirements and specifications, • installing the pump, • using the pump, •...

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