Agilent Technologies 1260 User Manual
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Agilent Technologies 1260 User Manual

Infinity diode array and multiple wavelength detector
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Agilent 1260 Infinity
Diode Array and Multiple
Wavelength Detector
User Manual
Agilent Technologies

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Summary of Contents for Agilent Technologies 1260

  • Page 1 Agilent 1260 Infinity Diode Array and Multiple Wavelength Detector User Manual Agilent Technologies...
  • Page 2 Use, duplication or dis- closure of Software is subject to Agilent For Research Use Only Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 3 In This Guide… In This Guide… This manual covers the Agilent 1260 Infinity Diode Array and Multiple Wavelength Detector modules: • G1315C - 1260 DAD VL+ • G1365C - 1260 MWD • G1315D - 1260 DAD VL • G1365D - 1260 MWD VL...
  • Page 4 This chapter provides information on parts for maintenance. 12 Identifying Cables This chapter provides information on cables used with the 1200 series of HPLC modules. 13 Appendix This chapter provides addition information on safety, legal and web. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 5: Table Of Contents

    4 LAN Configuration What you have to do first TCP/IP parameter configuration Configuration Switch Initialization mode selection Link configuration selection Automatic Configuration with BootP Storing the settings permanently with Bootp Manual Configuration Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 6 Intensity Test Holmium Oxide Test Spectral flatness test ASTM Noise Test Cell Test Using the Built-in Test Chromatogram Wavelength Verification and Recalibration Test Chromatogram Diagnosis Information on Agilent ChemStation D/A Converter (DAC) Test Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 7 Prep Flow Cell - Quartz Nano Flow Cells High Pressure Flow Cell Accessory Kits 12 Identifying Cables Cable Overview Analog Cables Remote Cables BCD Cables CAN/LAN Cables Agilent 1200 module to PC Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 8 Contents 13 Appendix General Safety Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Radio Interference Sound Emission UV-Radiation Solvent Information Declaration of Conformity for HOX2 Filter Agilent Technologies on Internet Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 9: Introduction

    Agilent 1260 Infinity DAD and MWD User Manual Introduction Introduction to the Detector Optical System Early Maintenance Feedback (EMF) Instrument Layout Electrical Connections Serial Number Information (ALL) Rear view of the module Interfaces Interfaces Overview Setting the 8-bit Configuration Switch...

  • Page 10: Introduction To The Detector

    • built-in holmium oxide filter for fast wavelength accuracy verification, • built-in temperature control for improved baseline stability, • additional diagnostic signals for temperature and lamp voltage monitoring, For specifications, see “Performance Specifications” on page 38. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 11: Optical System

    In the spectrograph, light is being dispersed onto the diode array by a holographic grating. This allows simultaneous access to all wavelength information. Figure 1 Optical System of the Detector Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 12: Optical System

    — slit and shutter — in a simple and compact component. The slit width is directly controlled by the micro-processor of the instrument and can be set as method parameter. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 13 The diode array is a series of 1024 individual photodiodes and control circuits located on a ceramic carrier. With a wavelength range from 190 – 950 nm the sampling interval is < 1 nm. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 14: Early Maintenance Feedback (Emf)

    EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 15: Instrument Layout

    • the plastic layers help cushion the electronic and mechanical parts from physical shock, and • the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 16: Electrical Connections

    There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.

  • Page 17: Electrical Connections

    Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module) 00000 Serial number Rear view of the module Figure 2 Rear View of Detector Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 18: Interfaces

    G1364C FC-AS G1330B μ CAN-DC- OUT for CAN G1364D FC- slaves G1367E HiP ALS G1377A HiP micro ALS G2258A DL ALS G4226A ALS Detectors G1314B VWD VL G1314C VWD VL+ G1314E/F VWD Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 19: Interfaces

    • LAN connector as interface to the control software • RS-232C as interface to a computer • REMOTE connector as interface to other Agilent products • Analog output connector(s) for signal output Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 20: Interfaces Overview

    • one start bit and one stop bit are always used (not selectable). The RS-232C is designed as DCE (data communication equipment) with a 9-pin male SUB-D type connector. The pins are defined as: Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 21 Direction Function Ground Figure 3 RS-232 Cable Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s main board. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 22 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
  • Page 23 (L) Request to start injection cycle (for example, by start key on any module). Receiver is the autosampler. Special Interfaces Some modules have module specific interfaces/connectors. They are described in the module documentation. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 24: Setting The 8-Bit Configuration Switch

    Location of Configuration Switch To perform any LAN configuration, SW1 and SW2 must be set to OFF. For details on the N O T E LAN settings/configuration refer to chapter “LAN Configuration”. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 25: Setting The 8-Bit Configuration Switch

    Revert to Default Data (Coldstart) Legend: 0 (switch down), 1 (switch up), x (any position) When selecting the mode TEST, the LAN settings are: Auto-Negotiation & Using Stored. N O T E Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 26 • for boot/test modes DIPS 1+2 must be UP plus required mode Switch settings provide configuration parameters for GPIB address, serial communication protocol and instrument specific initialization procedures. With the introduction of the Agilent 1260 Infinity, all GPIB interfaces have been removed. N O T E The preferred communication is LAN.
  • Page 27 Data Parity Bits Reserved Reserved TEST/BOOT RSVD RSVD RSVD The LAN settings are done on the LAN Interface Card G1369A/B. Refer to the N O T E documentation provided with the card. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 28: Communication Settings For Rs-232C

    Baud Rate Switches Baud Rate 9600 9600 1200 14400 2400 19200 4800 38400 Table 8 Data Bit Settings (without on-board LAN) Switch 6 Data Word Size 7 Bit Communication 8 Bit Communication Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 29 No Parity Odd Parity Even Parity One start bit and one stop bit are always used (not selectable). Per default, the module will turn into 19200 baud, 8 data bit with no parity. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 30: Special Settings

    It only uses basic functions of the operating system for example, for communication. In this mode the main firmware can be loaded (using update utilities). Table 10 Boot Resident Settings (without on-board LAN) Mode Select TEST/BOOT No LAN TEST/BOOT Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 31 If you use the following switch settings and power the instrument up again, a forced cold start has been completed. Table 11 Forced Cold Start Settings (without on-board LAN) Mode Select TEST/BOOT No LAN TEST/BOOT Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 32 Introduction Setting the 8-bit Configuration Switch Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 33: Site Requirements And Specifications

    Agilent 1260 Infinity DAD and MWD User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Specifications Specification Conditions This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies...

  • Page 34: Site Requirements

    ➔ Make sure the power connector of the instrument can be easily reached and unplugged. ➔ Provide sufficient space behind the power socket of the instrument to unplug the cable. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 35 Never operate your instrumentation from a power outlet that has no ground connection. ➔ Never use a power cord other than the Agilent Technologies power cord designed for your region. Use of unsupplied cables WA R N I N G Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury.
  • Page 36 ➔ If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 37: Physical Specifications

    Up to 2000 m (6562 ft) Non-operating altitude Up to 4600 m (15091 ft) For storing the module Safety standards: IEC, CSA, Installation Category II, Pollution Degree 2 For indoor use only. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 38: Performance Specifications

    Wavelength bunching 1 – 400 nm Programmable in steps of 1 nm Slit width 1, 2, 4 , 8, 16 nm Programmable slit Diode width < 1 nm Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 39 Agilent Instant Pilot (G4208A) For 1260 systems: • B.02.11 or above For other systems: • B.02.09 or above Analog outputs Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 40 Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter. Housing All materials recyclable. Others Electronic temperature control (ETC) for the complete optical unit Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 41: Specification Conditions

    Time Constant versus Response Time According to ASTM E1657-98 „Standard Practice of Testing Variable-Wavelength Photometric Detectors Used in Liquid Chromatography” the time constant is converted to response time by multiplying by the factor 2.2. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 42 Site Requirements and Specifications Performance Specifications Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 43: Installing The Module

    Agilent 1260 Infinity DAD and MWD User Manual Installing the Module Unpacking the Detector Damaged Packaging Delivery Checklist Optimizing the Stack Configuration Two Stack Configuration Installing the Detector Flow Connections to the Detector Setting up the LAN access This chapter gives information about the preferred stack setup for your system and the installation of your module.

  • Page 44: Unpacking The Detector

    Unpacking the Detector Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. "Defective on arrival" problems C A U T I O N If there are signs of damage, please do not attempt to install the module.

  • Page 45: Delivery Checklist

    1 (installed) G1315C/G1365C only Power cable Cross-over network cable Twisted pair network cable Flow cell As ordered User Manual on Doumentation CD (part of the shipment - not module specific) Accessory kit (p/n G1315-68755) Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 46: Optimizing The Stack Configuration

    If your detector is part of a complete Agilent 1200 Series system, you can ensure optimum performance by installing the following configuration. This configuration optimizes the system flow path, ensuring minimum delay volume. Figure 6 Recommended Stack Configuration for 1260 (Front View) Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 47 Installing the Module Optimizing the Stack Configuration Figure 7 Recommended Stack Configuration (Rear View) Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 48: Two Stack Configuration

    A slightly longer capillary is required between the pump and autosampler. (See Figure 8 on page 48 and Figure 9 on page 49). Figure 8 Recommended Two Stack Configuration for 1260 (Front View) Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 49 Installing the Module Optimizing the Stack Configuration Figure 9 Recommended Two Stack Configuration for 1260 (Rear View) Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 50: Installing The Detector

    “Performance Specifications” on page 38 will not recognize modules. For G1315C and G1365C assure that the CompactFlash Card is installed in the rear of the N O T E module (required for operation). Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 51 In multi-detector configurations the LAN of the G1315C/D and G1365C/D must be used N O T E due to its higher data load. 7 Connect the analog cable(s) (optional). 8 Connect the APG remote cable (optional) for non-Agilent 1200 Series instruments. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 52 The module is turned off when the line power switch is protruding and the green light is off. The module was shipped with default configuration settings. To change these settings see N O T E “Configuration Switch” on page 60. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 53: Flow Connections To The Detector

    Some types of the Agilent deuterium lamps show a light ring during operation. This is not harmful, refer to “UV-Radiation” on page 272. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 54 Press the release button and open the flow cell door. gain access to the flow cell area. Insert the flow cell. Connect the flow cell capillaries to the capillary holder (top is inlet, bottom is outlet). Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 55 Remove the flow cell and establish a flow and observe for Insert the flow cell, close the cover and replace the front leaks. cover. The installation of the detector is complete now. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 56: Setting Up The Lan Access

    Installing the Module Setting up the LAN access Setting up the LAN access Please follow the instructions in “LAN Configuration” on page 57 Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 57: Lan Configuration

    Agilent 1260 Infinity DAD and MWD User Manual LAN Configuration What you have to do first TCP/IP parameter configuration Configuration Switch Initialization mode selection Link configuration selection Automatic Configuration with BootP About Agilent BootP Service How BootP Service Works Situation: Cannot Establish LAN Communication...

  • Page 58: What You Have To Do First

    58) to • the PC network card using a crossover network cable (point-to-point) or • a hub or switch using a standard LAN cable. Figure 13 Location of LAN interface and MAC label Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 59: Tcp/Ip Parameter Configuration

    TCP/IP parameters after power-on. The parameters may be derived from a Bootp cycle, non-volatile memory or initialized with known default values. The initialization mode is selected by the configuration switch, see Table 18 on page 65. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 60: Configuration Switch

    Initialization (‘Init’) Mode Bootp, all switches down. For details see “Initialization mode selection” on page 61 Link Configuration speed and duplex mode determined by auto-negotiation, for details see “Link configuration selection” on page 65 Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 61: Initialization Mode Selection

    Bootp Server. The parameters obtained become the active parameters immediately. They are not stored to the non-volatile memory of the module. Therefore, the parameters are lost with the next power cycle of the module. Figure 15 Bootp (Principle) Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 62 Use the initialization mode Bootp & Store carefully, because writing to the non-volatile N O T E memory takes time. Therefore, when the module shall obtain its parameters from a Bootp Server every time it is powered on, the recommended initialization mode is Bootp! Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 63 The TCP/IP connection will be established using these parameters. The parameters were configured previously by one of the described methods. Figure 17 Using Stored (Principle) Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 64 In the Using Default mode, the parameters stored in the memory of the module are not N O T E cleared automatically. If not changed by the user, they are still available, when switching back to the mode Using Stored. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 65: Link Configuration Selection

    Link Configuration speed and duplex mode determined by auto-negotiation manually set to 10 Mbps, half-duplex manually set to 10 Mbps, full-duplex manually set to 100 Mbps, half-duplex manually set to 100 Mbps, full-duplex Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 66: Automatic Configuration With Bootp

    BootP Service is installed on a server or PC on the LAN to provide central administration of IP addresses for Agilent instruments on a LAN. The BootP service must be running TCP/IP network protocol and cannot run a DHCP server. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 67: How Bootp Service Works

    • Does the Firewall block the BootP service? Add the BootP service as an exception. • Is the LAN Interface using the BootP-mode instead of "Using Stored" or "Using Default" modes? Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 68: Installation Of Bootp Service

    9 The Destination Folder selection screen appears. Install BootP to the default folder or click Browse to choose another location. Click Next. The default location for installation is: C:\Program Files\Agilent\BootPService\ 10 Click Install to begin installation. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 69 15 Remove the DVD from the drive. This completes installation. 16 Start the BootP service. On the Windows® desktop, select Start > Control Panel > Services. Select the Agilent BootP Service and click Start. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 70: Two Methods To Determine The Mac Address

    (:) and usually begins with the letters AD, see Figure 12 on page 58 and Figure 13 on page 58. 3 Turn on the instrument. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 71: Assigning Ip Addresses Using The Agilent Bootp Service

    5 Close the log file before turning on another instrument. 6 Uncheck the Do you want to log BootP requests? box after configuring instruments to avoid having the logfile use up excessive disk space. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 72 3 Click Edit BootP Addresses... The Edit BootP Addresses screen appears. 4 Click Add... The Add BootP Entry screen appears. Figure 21 Enable BootP logging Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 73 11 Use the PING utility to verify connectivity by opening a command window and typing: Ping 10.1.1.101 for example. The Tab File is located at C:\Documents and Settings\All Users\Application Data\Agilent\BootP\TabFile Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 74: Changing The Ip Address Of An Instrument Using The Agilent Bootp Service

    1 From the Windows control panel, select Administrative Tools > Services. The Services screen appears. Figure 22 Windows Services screen 2 Right-click Agilent BootP Service. 3 Select Stop. 4 Close the Services and Administrative Tools screen. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 75 1 In the Windows control panel, select Administrative Tools > Services. The Services screen appears, see Figure 22 on page 74. 2 Right-click Agilent BootP Service and select Start. 3 Close the Services and Administrative Tools screens. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 76: Storing The Settings Permanently With Bootp

    8 Change the settings of the module’s Configuration Switch to “Using Stored” mode, see Table 16 on page 61. 9 Power cycle the module. The module can be accessed now via LAN without the Agilent Bootp Service. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 77: Manual Configuration

    Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing Figure 24 Manual Configuration (Principle) Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 78: With Telnet

    Handheld Controller, or the default IP address (see “Configuration Switch” on page 60). When the connection was established successfully, the module responds with the following: Figure 26 A connection to the module is made Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 79 Then press [Enter], where parameter refers to the configuration parameter you are defining, and value refers to the definitions you are assigning to that parameter. Each parameter entry is followed by a carriage return. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 80 Initialization mode is Using Stored active TCP/IP settings stored TCP/IP settings in non-volatile memory connected to PC with controller software (e.g. Agilent ChemStation), here not connected Figure 29 Telnet - Change IP settings Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 81 If the Initialization Mode Switch is changed now to “Using Stored” mode, the instrument N O T E will take the stored settings when the module is re-booted. In the example above it would be 134.40.27.99. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 82: With The Instant Pilot (G4208A)

    Instant Pilot - LAN Configuration 5 Press the Edit button (only visible if not in Edit mode), perform the required changes and press the Done button. 6 Leave the screen by clicking Exit. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 83: Using The Detector

    Agilent 1260 Infinity DAD and MWD User Manual Using the Detector Setting up an Analysis Before Using the System Requirements and Conditions Optimization of the System Preparing the HPLC System Running the Sample and Verifying the Results Special Settings of the Detector...

  • Page 84: Setting Up An Analysis

    (when available in the system) and the pump. Volatile ingredients will evaporate to some extend. Therefore priming of the pumping system is required before starting an application. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 85 2 Flush all tubes with at least 30 ml of solvent. 3 Set flow to required value of your application and close the purge valve. Pump for approximately 10 minutes before starting your application. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 86: Requirements And Conditions

    Zorbax Eclipse XDB C18, 150 mm x 4.6 mm, 5 µm (p/n 993967-906) Standard: Agilent isocratic checkout sample (p/n 01080-68704). This 0.5 mL ampoule contains 0.15 wt.% dimethylphthalate, 0.15 wt.% diethylphthalate, 0.01 wt.% biphenyl, 0.03 wt.% o-terphenyl in methanol. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 87 Table 22 Conditions Flow 1.5 ml/minute Stoptime 8 minutes Solvent 100% (30% water/70% Acetonitrile) Temperature Ambient Wavelength sample 254 nm (4 nm bandwidth) reference 360 nm (100 nm bandwidth) Injection Volume 1 µl Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 88 The exact profile of the chromatogram will depend on the chromatographic conditions. Variations in solvent quality, column packing, standard concentration and column temperature will all have a potential effect on peak retention and response. Figure 32 Typical Chromatogram with UV-detector Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 89: Optimization Of The System

    The settings used for this analysis are specific for this purpose. For other applications the system can be optimized in various ways. Please refer to the section “Optimizing the Detector” on page 113. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 90: Preparing The Hplc System

    ChemStation screen should look like shown in Figure below. The System status is red (Not Ready). Figure 33 Initial ChemStation screen (Method and Run Control) Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 91 (GUI). After some time, the pump, thermostatted column compartment and detector module will turn to green. Figure 34 Turning on the HPLC Module Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 92 Table 23 Baseline drift after lamp turn on (example from Figure above) Time [minutes] Drift [mAU/hr] 17 - 20 27 - 30 37 - 40 47 - 50 57 - 60 < 0.2 Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 93 8 Click on the Load Method button and select DEF_LC.M and press OK. Alternative double-click on the method in the method window. The default LC method parameters are transferred into the modules. Figure 36 Loading Default LC Method Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 94 9 Click on the module icons (see Figure below) and open the Setup of these modules. Figure on page 95 shows the detector settings (do not change the detector parameters at this time). Figure 37 Open the module menu Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 95 • time table for programmable actions during the run. NOTE: The Agilent G1315C/D and G1365C/D time table can contain a maximum of 60 rows. 11 Pump the water/acetonitrile (30/70 %) mobile phase through the column for 10 minutes for equilibration. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 96 97) shows both, the pump pressure and the detector absorbance signals. Pressing Adjust the signals can be reset to the offset value and Balance would do a balance on the detector. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 97 13 If both baselines are stable, set the Y-range for the detector signal to 100 mAU. If you start with a new UV-lamp for the first time, the lamp may show initial drift for some N O T E time (burn-in effect). Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 98 Figure 40 Sample Information 15 Fill the content of an isocratic standard sample ampoule into a vial and seal the vial with a cap and place the vial into autosampler tray (position #1). Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 99: Running The Sample And Verifying The Results

    Figure 41 Chromatogram with Isocratic Test Sample Information about using the Data Analysis functions can be obtained from the Using your N O T E ChemStation manual supplied with your system. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 100: Special Settings Of The Detector

    V full scale, “Analog Output Settings” on page 107 • Automatic Turn On: lamps can be programmed (detector must be on for this). • Help: online help. Figure 42 Detector control settings Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 101: Configuration Settings

    N O T E Temperature Control to off. This will lower the optical unit and flow cell temperature by some degree C. For more details see “Principle of Temperature Control” in the Service Manual. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 102: Online Spectra (Dad Only)

    Special Settings of the Detector Online Spectra (DAD only) 1 To view the online spectra during the run select Online Spectra. Figure 44 Online Spectra Window 2 Change the absorbance and wavelength range according your needs. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 103: Run Recovery Settings

    When during recovery an error “Method/Sequence stopped” appears, the instrument N O T E logbook shows an entry “No Run data available in device“. In this case refer to “No Run Data Available In Device” on page 158. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 104: Automated Run Recovery In Case Of Temporary Communication Failures

    • Elapsed run time continues at actual time • Spectra counter continues • Data continues to be stored on PC and on card • ChemStation tries already to add missing data (depends on the data load). Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 105 If the detector status window is not opened, you will realize only the Power Fail error and N O T E the long Run In Progress information until the data is recovered from disk. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 106: Manual Run Recovery In Case Of Permanent Communication Failures

    When during recovery an error “Method/Sequence stopped” appears, the instrument N O T E logbook shows an entry “No Run data available in device“. In this case refer to “No Run Data Available In Device” on page 158 Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 107: Analog Output Settings

    Zero Offset Limits: 1 to 99% in steps of 1% Attenuation Limits: 0.98 to 2000 mAU at discrete values for either 100 mV or 1 V full scale Figure 45 Analog Output Settings 3 Change the values if required. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 108: Spectrum Settings (Dad Only)

    2 In the section Spectrum click on the drop-down list and chose a parameter. Table 25 on page 109 shows the possible parameters. 3 Change the Range, Step width and Threshold according to your needs. Figure 46 Spectra Settings Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 109 Limits: 0.001 to 1000.00 mAU in steps of 0.001 mAU. Usable for modes Apex + Baselines, Apex + Slopes + Baselines and All in Peak Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 110: Peakwidth Settings

    Limits: When you set the peak width (in minutes), the corresponding response time is set automatically and the appropriate data rate for signal and spectra acquisition is selected as shown in the table below. Figure 47 Peakwidth Setting Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 111 >0.01 G1315C/D and G1365C/D >0.03 G1315C/D and G1365C/D >0.05 G1315C/D and G1365C/D >0.10 G1315C/D and G1365C/D >0.20 1.25 G1315C/D and G1365C/D >0.40 0.62 G1315C/D and G1365C/D >0.85 16.0 0.31 G1315C/D and G1365C/D Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 112: Slit Settings

    The smaller the optical bandwidth the higher the spectral resolution. To set the slit width, display the drop-down list and select an appropriate slit width from the list. Figure 48 Slit Settings Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 113: Margin For Negative Absorbance Settings

    The higher the value the greater the baseline noise. Set this value only if you expect N O T E negative absorbance greater than -100 mAU. Optimizing the Detector Additional theoretical information can be found in chapter “How to optimize the Detector” on page 115. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 114: Special Setups With Multiple Dad-Mwds

    If you have similar detectors in the same system, the G1315C/D will be automatically always detector 2 while the G1315A/B is detector 1 independent from its location in the stack. This cannot be changed. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 115: How To Optimize The Detector

    Agilent 1260 Infinity DAD and MWD User Manual How to optimize the Detector Introduction Optimization Overview Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Flow Cell Path Length Peak width (response time) Sample and Reference Wavelength and Bandwidth Slit Width Optimizing Spectral Acquisition (DAD only)

  • Page 116: Introduction

    How to Get the Best Detector Performance The information below will guide you on how to get the best detector performance. Follow these rules as a start for new applications. It gives rules-of-thumb for optimizing detector parameters. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 117: Optimization Overview

    Select the reference wavelength with broad bandwidth (30...100 nm) • baseline drift due to RI effects. wavelength range where your analytes have little or no absorbance (e.g. sample at 254 nm, reference at 320 nm). Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 118 • Set step to 4 nm for normal use; set small step (and slit width) if high resolution of spectra with fine structure is wanted. Figure 50 Choosing a Flow Cell in HPLC Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 119: Optimizing For Sensitivity, Selectivity, Linearity And Dispersion

    To determine the volume of your peaks, take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3). Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 120 G1315-60024) Semi-nano flow cell kit, 10 mm, 500 nL, 5 MPa (p/n 10.00 ±0.02 mm 10/10 G1315-68724) Nano flow cell kit, 10 mm, 80 nL, 5 MPa (p/n G1315-68716) 6.00 ±0.02 mm Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 121: Peak Width (Response Time)

    (response time) by factor of two from the recommended setting (over-filtering) will reduce peak height by about 20 % and reduce baseline noise by a factor of 1.4. This gives you the best possible signal-to-noise ratio, but may affect peak resolution. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 122 >0.01 G1315C/D and G1365C/D >0.03 G1315C/D and G1365C/D >0.05 G1315C/D and G1365C/D >0.10 G1315C/D and G1365C/D >0.20 1.25 G1315C/D and G1365C/D >0.40 0.62 G1315C/D and G1365C/D >0.85 16.0 0.31 G1315C/D and G1365C/D Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 123: Sample And Reference Wavelength And Bandwidth

    If you work with high concentrations, you may get better linearity above 1.5 AU by setting the sample wavelength to a valley in the spectrum, like 225 nm for anisic acid. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 124 4 nm wide band. The signal-to-noise ratio for a 30 nm bandwidth is twice that for a 4 nm bandwidth in our example. Figure 54 Influence of Bandwidth on Signal and Noise Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 125 With this technique, PTH-amino acids can be quantified in the low picomole range even in a gradient analysis. Figure 55 Gradient Analysis of PTH-Amino Acids (1 pmol each), with and without Reference Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 126: Slit Width

    Benzene at 1, 4 and 16 nm slit width (principle) A wide slit uses more of the light shining through the flow cell. This gives lower baseline noise as shown in Figure 57 on page 127. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 127 Use a wide (8 or 16 nm) slit when your sample contains very small concentrations. Always use signals with bandwidth at least as wide as the slit width. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 128: Optimizing Spectral Acquisition (Dad Only)

    (about -100 mAU). In some special case, for example, when gradient with absorbing solvents are used, the baseline may drift to more negative values. Only for such cases, increase the margin for negative absorbance to avoid overflow of the analog-to-digital converter. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 129: Optimizing Selectivity

    It would therefore be impossible, with a conventional variable wavelength detector, to detect hydrochlorothiazide quantitatively when caffeine is present. Figure 58 Wavelength Selection for Peak Suppression Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 130 The trade-off for this procedure is a loss in sensitivity. The sample signal decreases by the absorbance at the reference wavelength relative to the signal wavelength. Sensitivity may be decreased by as much as 10–30 %. Figure 59 Peak Suppression Using Reference Wavelength Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 131: Ratio Qualifiers For Selective Detection Of Compound Classes

    An example is shown in Figure 60 on page 131 and Figure 61 on page 132. Figure 60 Wavelength Selection for Ratio Qualifiers Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 132 249 and 224 nm was within this range, is the signal plotted. Of all four peaks, only the third fulfilled the criterion (Figure 61 on page 132). The others were not plotted. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 133: Troubleshooting And Diagnostics

    Agilent 1260 Infinity DAD and MWD User Manual Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Status Indicators Power Supply Indicator Module Status Indicator User Interfaces Agilent Lab Advisor Software This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces.

  • Page 134: Overview Of The Module's Indicators And Test Functions

    The module uses the deuterium alpha and beta emission lines for wavelength calibration (see “Wavelength Verification and Recalibration” on page 183). Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 135: Status Indicators

    Figure 62 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch. When the indicator is illuminated (green) the power is ON. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 136: Module Status Indicator

    (modules without on-board LAN) indicator indicates that the module is in boot loader mode (e.g. during update of main firmware). In such a case try to re-boot the module or try a cold-start. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 137: User Interfaces

    Test Functions avaible vs. User Interface Test Diagnostic Software ChemStation Instant Pilot G4208A Selftest Filter Slit D/A Converter Test Chromatogram Yes (*) Wavelength Calibration Lamp Intensity Holmium Cell Dark Current requires a command via command line Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 138: Agilent Lab Advisor Software

    Agilent Lab Advisor software may differ from the descriptions in this manual. For details refer to the Agilent Lab Advisor software help files. This manual provides lists with the names of Error Messages, Not Ready messages, and other common issues. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 139: Error Information

    Agilent 1260 Infinity DAD and MWD User Manual Error Information What Are Error Messages General Error Messages Timeout Shut-Down Remote Timeout Synchronization Lost Leak Sensor Short Leak Sensor Open Compensation Sensor Open Compensation Sensor Short Fan Failed Leak Additional Information...
  • Page 140 DSP Not Running No Run Data Available In Device Instrument Logbook This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 141: What Are Error Messages

    In the event of such a failure, the red status indicator at the front of the module is switched on, and an entry is written into the module logbook. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 142: General Error Messages

    Check the logbook for the occurrence and A not-ready condition was present during a source of a not-ready condition. Restart the sequence or multiple-injection run for a analysis where required. period longer than the timeout threshold. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 143: Shut-Down

    Exchange the remote cable. Defective remote cable. Check the instrument for defects (refer to the Defective components in the instrument instrument’s documentation). showing the not-ready condition. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 144: Synchronization Lost

    • Please contact your Agilent service Leak sensor incorrectly routed, being representative. pinched by a metal component. • Correct the routing of the cable. • If cable defective, exchange the leak sensor. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 145: Leak Sensor Open

    If the resistance across the sensor increases above the upper limit, the error message is generated. Probable cause Suggested actions Please contact your Agilent service Defective main board. representative. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 146: Compensation Sensor Short

    This limit is given by 2 revolutions/second for longer than 5 seconds. Probable cause Suggested actions Please contact your Agilent service Fan cable disconnected. representative. Please contact your Agilent service Defective fan. representative. Please contact your Agilent service Defective main board. representative. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 147: Leak

    Probable cause Suggested actions Ensure all fittings are tight. Loose fittings. Exchange defective capillaries. Broken capillary. Exchange flow cell components. Leaking flow cell. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 148: Additional Information

    The leak drain for the detector can be connected to the detector's leak drain fitting, and taken to waste separately. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 149: Open Cover

    Probable cause Suggested actions The top foam was removed during operation. Please contact your Agilent service representative. Please contact your Agilent service Foam not activating the sensor. representative. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 150: Detector Error Messages

    Ensure the visible lamp connector is seated Lamp disconnected. firmly. Exchange the visible lamp. Defective visible lamp. Please contact your Agilent service Defective connector or cable. representative. Please contact your Agilent service Defective power supply. representative. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 151: Visible Lamp Voltage

    If the leakage current exceeds the upper limit, the error message is generated. Probable cause Suggested actions Please contact your Agilent service Defective PDA/optical unit. representative. Please contact your Agilent service Defective connector or cable. representative. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 152: Uv Lamp Current

    Probable cause Suggested actions Exchange the UV lamp. Defective UV lamp or non-Agilent lamp. Please contact your Agilent service Defective detector main board. representative. Please contact your Agilent service Defective power supply. representative. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 153: Uv Ignition Failed

    Ensure the lamp is connected. Lamp disconnected. Exchange the UV lamp. Defective UV lamp or non-Agilent lamp. Please contact your Agilent service Defective detector main board. representative. Please contact your Agilent service Defective power supply. representative. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 154: Uv Heater Current

    ROM, the error message is generated. Probable cause Suggested actions Please contact your Agilent service Defective connector or cable. representative. Please contact your Agilent service Defective PDA/optical unit. representative. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 155: Holmium Oxide Test Failed

    Probable cause Suggested actions Please contact your Agilent service Dirty or defective sensor. representative. Links: Verify that the ambient conditions are within the Detector is exposed to illegal ambient allowed range. conditions. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 156: Illegal Value From Air Inlet Temperature Sensor

    ROM, the error message is generated. Probable cause Suggested actions Recalibrate the detector. The detector is new. Please contact your Agilent service The detector has been repaired. representative. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 157: Heater Failed

    • Verify that the reproducibility of your results Ambient conditions have changed too much is not affected. during the run, so that optimum results may not be guaranteed. • Expose the detector to more stable ambient conditions. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 158: Dsp Not Running

    (e.g full data rate at 80 Hz plus full spectra plus all signals) during data buffering. Probable cause Suggested actions • Correct communication problem. CompactFlash Card is full. • Reduce data rate. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 159: Instrument Logbook

    Detector Error Messages Instrument Logbook Figure 63 Instrument Logbook The logbook does not indicate a communication loss (power fail). It just shows the N O T E recovering (Power on, Lamps on). Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 160 Error Information Detector Error Messages Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 161: Test Functions

    Agilent 1260 Infinity DAD and MWD User Manual Test Functions Self-test Filter Test Filter Test Evaluation Slit Test Slit Test Evaluation Dark-Current Test Dark-Current Test Evaluation Intensity Test Intensity Test Evaluation Holmium Oxide Test Holmium Oxide Test Evaluation Spectral flatness test...

  • Page 162: Self-Test

    Setup of the self test is done in the Self Test dialog box. Select either Single Test or Repetitive Tests. Check the ASTM Noise Test checkbox to include the noise test in the self test. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 163 Test Functions Self-test Figure 64 Self-test Results (report) For details refer to the individual tests on the following pages. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 164: Filter Test

    The test passes successfully, if the two maxima resulting from the edge of the filter assembly (during filter movement) are seen, and the absorbance maximum of holmium oxide is within the limits. Figure 65 Filter Test Results (report) Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 165: Filter Test Evaluation

    Defective filter motor. Holmium Oxide Maximum out of Limits Probable cause Suggested actions Install the holmium oxide filter. Holmium oxide filter not installed. Exchange the holmium oxide filter. Dirty or contaminated filter. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 166: Slit Test

    Old or non-Agilent lamp. the lamp if old or defective. Exchange the slit assembly. Defective slit assembly (stray light). Exchange the detector main board. Defective detector main board. Exchange the optical unit. Defective optical unit. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 167: Dark-Current Test

    (see Figure 66 page 168). The leakage current (represented in counts) for each diode should fall within the limits (red bands) shown in the plot (see Figure 66 page 168). Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 168: Dark-Current Test Evaluation

    Test Functions Dark-Current Test Dark-Current Test Evaluation Dark-Current Test Evaluation Limit: 0... 12000 counts Figure 66 Dark-Currrent Test Results (report) Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 169 “Slit Test” on page 166 (part of the Defective slit assembly (stray light). “Self-test” on page 162). Exchange the detector main board. Defective detector main board. Exchange the optical unit. Defective PDA/optical unit. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 170: Intensity Test

    The shape of the intensity spectrum is primarily dependent on the lamp, grating, and diode array characteristics. Therefore, intensity spectra will differ slightly between instruments. Figure 67 on page 171 shows a typical intensity test spectrum. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 171: Intensity Test Evaluation

    (see Figure 67 on page 171). Figure 67 Intensitiy Test Results (report) Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 172 Clean optical components with alcohol and Dirty or contaminated optical components lint-free cloth or replace the parts. (achromat, windows). Exchange the lamp. Old or non-Agilent lamp. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 173 If the intervals of lamp replacements are getting shorter, the Agilent service should check the optical unit for contaminated components in the light path (coupling lens, source lens, cell support assembly and flow cell windows). Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 174: Holmium Oxide Test

    The test is evaluated by the instrument, and the measured maxima are displayed automatically. The test fails if one or more of the maxima lies outside of the limits (see Figure 68 on page 175). Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 175 Test Functions Holmium Oxide Test Figure 68 Holmium Oxide Test Results (report) Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 176 Clean optical components with alcohol and Dirty or contaminated optical components lint-free cloth or replace the parts (see (achromat, windows). “Intensity Test” on page 170). Exchange the UV lamp. Old or non-Agilent lamp. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 177: Spectral Flatness Test

    Suggested actions • Run the Intensity Test. Old or non-Agilent lamp. • Exchange the lamp if old or defective. Exchange the DAM board. Defective DAM board Exchange the optical unit. Defective optical unit. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 178: Astm Noise Test

    ASTM Noise Test Evaluation Limit is ± 0.02 mAU Test Failed Probable cause Suggested actions Allow lamp to warm-up for at least 1 hour. Insufficient lamp warm-up time Exchange the lamp. Old or non-Agilent lamp. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 179: Cell Test

    This test should be performed inititially with a new detector/flow cell. The values should be N O T E kept for later reference/comparison. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 180: Cell Test Evaluation

    Suggested actions Ensure the flow cell is filled with water, and free Absorbing solvent or air bubble in flow cell. from air bubbles. Exchange the flow cell windows. Dirty or contaminated flow cell. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 181: Using The Built-In Test Chromatogram

    Tools selection. 3 Open the test chromatogram screen 4 Turn the Test Chromatogram on. 5 Change to the detector's Module Service Center and add the detector signal to the Signal Plot window. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 182 Test Chromatogram with Agilent LabAdvisor 7 To stop the test chromatogram enter in the command line: STOP The test chromatogram is switched off automatically at the end of a run. N O T E Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 183: Wavelength Verification And Recalibration

    • after a major repair, like processor board or optical unit exchange, see also “Replacing the Module’s Firmware” on page 229. After calibration, the holmium oxide test (see Figure 68 on page 175) provides verification of wavelength accuracy at three additional wavelengths. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 184: Test Chromatogram

    4.5 min 4 sec 9 min 8 sec 18 min 16 sec 36 min Table 32 Test Chromatogram - Peak Heights Peak Absorbance (approx.) 38 mAU 100 mAU 290 mAU 20 mAU Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 185: Test Chromatogram With Agilent Chemstation

    3 Reply: RA 00000 SIMU:DFLT 4 Start the run. On completion of the run, or when the run is stopped, the test chromatogram is deactivate. Figure 72 Test Chromatogram on Agilent ChemStation (response time 2 seconds) Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 186: Diagnosis Information On Agilent Chemstation

    Filter movements, filter moves reset log Flow Cell Available with ID-tag flow cells only. Product number, production date, pathlength, max pressure, serial number, volume, last cell test Micro Slit Slit movements, filter moves reset log Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 187 Test Functions Diagnosis Information on Agilent ChemStation Figure 73 Diagnosis screen in Agilent ChemStation (detailed view) Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 188: D/A Converter (Dac) Test

    Running the test with Agilent LabAdvisor 1 Run the D/A Converter (DAC) Test (for further information see Online-Help of user interface). Figure 74 D/A Converter (DAC) Test – Results Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 189: Test Failed

    The noise on the step should be less than 3 µV. Probable cause Suggested actions Check or replace the cable. Bad cable or grounding problem between detector and external device. Exchange the detector main board. Defective detector main board. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 190 Test Functions D/A Converter (DAC) Test Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 191: Maintenance

    Agilent 1260 Infinity DAD and MWD User Manual Maintenance Introduction to Maintenance Cautions and Warnings Overview of Maintenance Cleaning the Module Exchanging a Lamp Exchanging a Flow Cell Maintenance of Standard, Semi-Micro or Micro Flow Cell Maintenance of High Pressure Flow Cell...

  • Page 192: Introduction To Maintenance

    The module is designed for easy maintenance. Maintenance can be done from the front with module in place in the system stack. There are no serviceable parts inside. N O T E Do not open the module. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 193: Cautions And Warnings

    Repair work at the module can lead to personal injuries, e.g. shock hazard, when the cover is opened. ➔ Do not remove the metal top cover of the module. No serviceable parts inside. ➔ Only certified persons are authorized to carry out repairs inside the module. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 194 ➔ If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 195: Overview Of Maintenance

    A holmium or wavelength calibration Cleaning or exchange test should be performed after replacement. Leak sensor drying If leak has occurred. Check for leaks. Leak handling System If broken or corroded. Check for leaks. replacement Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 196: Cleaning The Module

    Liquid in the module electronics can cause shock hazard and damage the module. ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any fittings. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 197: Exchanging A Lamp

    Injury by touching hot lamp WA R N I N G If the detector has been in use, the lamp may be hot. ➔ If so, wait for lamp to cool down. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 198 Do not touch the glass bulb with your fingers. When replacing the Vis-lamp, assure that the Vis-lamp is Insert the lamp. Fix the screws and reconnect the lamp to inserted as shown (flat edge towards the deuterium connector. lamp). Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 199 Turn the lamp on and give the lamp 10 minutes to warm Perform a “Wavelength Verification and Recalibration” page 183 or a “Holmium Oxide Test” on page 174 to check the correct positioning of the UV-lamp. Perform an “Intensity Test” on page 170. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 200: Exchanging A Flow Cell

    218 Preparations Turn the lamp(s) off. Remove the front cover. Press the release buttons and remove the front cover to Open the flow cell cover. gain access to the flow cell area. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 201 Loosen the thumb screw and remove the flow cell outlet Remove the flow cell while pressing the flow cell holder. capillary (bottom) with the union. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 202 Next Steps: Perform a “Wavelength Verification and Recalibration” on page 183 or a “Holmium Oxide Test” on page 174 to check the correct positioning of the flow cell. Replace the front cover. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 203: Maintenance Of Standard, Semi-Micro Or Micro Flow Cell

    N O T E If the washers fall out of the window assembly, they must be inserted in the correct order with the Teflon® ring to prevent any leaks from the flow cell window. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 204 Orientation of Flow Cell Parts (“Standard Flow Cell” window assembly. page 234) Assemble the washers and the window assembly in N O T E correct order. Gaskets # 6 and #7 have different hole diameters. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 205 Press the window assembly onto the new or cleaned Insert a new gasket [6, 7] and the window assembly [1] quartz window. into the cell body. Assure, that the gasket is on the bottom. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 206 Insert the flow cell. Replace the front cover Perform a “Wavelength Verification and Recalibration” on page 183 or a “Holmium Oxide Test” on page 174 to check the correct positioning of the flow cell. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 207: Maintenance Of High Pressure Flow Cell

    The heat exchanger/capillary and the cell body can be fixed mirror symmetrically to have both capillaries routed to the bottom or to the top (depending on the routing of the capillaries to the column). Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 208 If you want to replace the gasket only, continue with step 8, “Maintenance of Standard, Semi-Micro or Micro Flow Cell” on page 203. Follow the procedure “Maintenance of Standard, Semi-Micro or Micro Flow Cell” on page 203 for reassembling. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 209: Replacing Capillaries On A Standard Flow Cell

    When retightening the fittings, make sure that they are carefully tightened (handtight plus 1/4 turn with a wrench). Otherwise damage of the flow cell body or blockage may result. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 210 4-mm wrench for the fitting." To replace the inlet capillary, use a 4-mm wrench for the Unscrew the cell body from the heat exchanger and the fitting. heat exchanger from the clamp unit. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 211 Take the new inlet capillary and bend it 90° about 35 mm Bend the capillary again by 90° as shown below. from its end. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 212 Insert the fixing screw, so that the capillary cannot leave Carefully insert the I.D. tag into the new heat exchanger. the grove. Shown is the default orientation. See Note at the beginning of this section. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 213 Insert the flow cell. Replace the front cover. Perform a “Wavelength Verification and Recalibration” page 183 or a “Holmium Oxide Test” on page 174 to check the correct positioning of the flow cell. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 214: Replacing Capillaries On A Semi-Micro And Micro Flow Cell

    When retightening the fittings, make sure that they are carefully tightened (handtight plus 1/4 turn with a wrench). Otherwise damage of the flow cell body or blockage may result. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 215 Then do a 1/4 turn with a 4-mm wrench. To replace the inlet capillary, use a 4-mm wrench for the Unscrew the cell body from the heat exchanger and the fitting. heat exchanger from the clamp unit. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 216 Fix the new heat exchanger to the clamp unit and the heat Fix the inlet capillary to the flow cell body handtight first. exchanger to the cell body. Then do a 1/4 turn with a 4-mm wrench. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 217 Insert the flow cell. Replace the front cover. Perform a “Wavelength Verification and Recalibration” page 183 or a “Holmium Oxide Test” on page 174 to check the correct positioning of the flow cell. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 218: Nano Flow Cell - Replacing Or Cleaning

    For details refer to the technical note that comes with the nano-flow cell kit. N O T E The quartz block can be cleaned with alcohol. DO NOT touch the inlet and outlet windows N O T E at the quartz block. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 219 Unscrew the capillaries from the flow cell. DO NOT use Using for example a toothpick, press on the plastic part the adapter at this time! and slide the quartz body out of the cell housing. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 220 Replace the cell seal assembly onto the quartz body. Slide the quartz body completely into the cell body to the Always use a new seal assembly to exclude damage front stop (use for example a toothpick). during disassembling. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 221 If no leak is observed, install the flow cell and you are ready to work. Make sure that the flow cell assembly is inserted correctly and fits perfectly in the optical unit (especially when PEEK capillaries are used). Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 222 0.7 Nm). It can be used to tight the capillary fittings at the flow cell body. The wrench has to be plugged into the adapter as shown in Figure 76 page 222. Figure 76 Wrench plus Torque Adapter Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 223: Cleaning Or Exchanging The Holmium Oxide Filter

    A slight change in the absorbance might be possible. Other components within the light path (lenses, windows, ...) are also changing their behavior over the time. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 224 While releasing the holder with a screw driver (at the N O T E top), carefully remove the holmium oxide filter. Do not scratch the holmium oxide filter. The holmium oxide filter can be cleaned with alcohol and a lint-free cloth. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 225 174 to check the proper function of the holmium oxide filter. Insert the flow cell, see “Exchanging a Flow Cell” on page 200. Replace the front cover. Turn on the flow. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 226: Correcting Leaks

    1 Use tissue to dry the leak sensor area and the leak pan. 2 Observe the capillary connections and the flow cell area for leaks and correct, if required. Figure 77 Observing for Leaks 3 Replace the front cover. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 227: Replacing Leak Handling System Parts

    3 Insert the leak funnel with the tubing in its position. 4 Insert the leak funnel into the leak funnel holder. Figure 78 Replacing Leak Handling System Parts 5 Replace the front cover. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 228: Replacing The Compactflash Card (G1315C/G1365C Only)

    1 Remove the CompactFlash card by pulling it out of its slot in the rear of the detector. 2 Install the new CompactFlash card into the slot. 3 Turn the detector ON. Figure 79 Replacing CompactFlash card Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 229: Replacing The Module's Firmware

    1 Download the required module firmware, the latest LAN/RS-232 FW Update Tool and the documentation from the Agilent web. • http://www.chem.agilent.com/scripts/cag_firmware.asp. 2 To load the firmware into the module follow the instructions in the documentation. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 230 A.06.xx or B.01.02 or above (main and modules resident). Otherwise the communication will not work. Conversion to / Not possible due to different hardware and electronic platform. emulation of G1315B or G1365B Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 231: Parts For Maintenance

    Agilent 1260 Infinity DAD and MWD User Manual Parts for Maintenance Overview of Maintenance Parts Standard Flow Cell Semi-Micro Flow Cell Parts Micro Flow Cell Prep Flow Cell - SST Prep Flow Cell - Quartz Nano Flow Cells High Pressure Flow Cell Accessory Kits This chapter provides information on parts for maintenance.

  • Page 232: Overview Of Maintenance Parts

    Twisted pair network cable, shielded, 7 m (for point to point connection) 01046-60105 Analog cable (BNC to general purpose, spade lugs) G1351-68701 Interface board (BCD) with external contacts and BCD outputs 01100-68700 CompactFlash Card Kit Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 233 Parts for Maintenance Overview of Maintenance Parts Figure 80 Maintenance Parts Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 234: Standard Flow Cell

    4 mm hexagonal wrench and seal kit 79883-68703 Window screw kit, includes 2 quartz windows, 2 compression washers, 2 window holders, 2 window screws and 10 spring washers Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 235 Parts for Maintenance Standard Flow Cell Figure 81 Standard Flow Cell Parts Gaskets # 6 and #7 have different hole diameters. N O T E Figure 82 Orientation of Spring Washers Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 236: Semi-Micro Flow Cell Parts

    Cell repair kit semi-micro, includes window screw kit, 4 mm hexagonal wrench and seal kits 79883-68703 Window screw kit, includes 2 quartz windows, 2 compression washers, 2 window holders, 2 window screws and 10 spring washers Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 237 Parts for Maintenance Semi-Micro Flow Cell Parts Figure 83 Semi-Micro Flow Cell Parts Gaskets # 6 and #7 have different hole diameters. N O T E Figure 84 Orientation of Spring Washers Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 238: Micro Flow Cell

    Cell repair kit semi-micro, includes window screw kit, 4 mm hexagonal wrench and seal kits 79883-68703 Window screw kit, includes 2 quartz windows, 2 compression washers, 2 window holders, 2 window screws and 10 spring washers Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 239 Parts for Maintenance Micro Flow Cell Figure 85 Micro Flow Cell Parts Gaskets # 6 and #7 have different hole diameters. N O T E Figure 86 Orientation of Spring Washers Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 240: Prep Flow Cell - Sst

    5062-2418 1/16” fittings and ferrules 10/pk G1315-27706 Cell body G1315-84901 Clamp unit G1315-84902 Handle for Clamp unit 0515-1056 Screw M 2.5, 4 mm lg for cell body/clamp Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 241 Prep Flow Cell - SST Figure 87 Prep Flow Cell - SST Parts Gaskets # 6 and #7 have different hole diameters. N O T E Figure 88 Orientation of Spring Washers Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 242: Prep Flow Cell - Quartz

    0.8/0.8 or 0.5/0.8 (inlet/outlet). Standard is 0.8/0.8. Depending on the system pressure (<30 mL/min) or bandbroadening, the inlet tubing might be changed to 0.5 mm. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 243 Parts for Maintenance Prep Flow Cell - Quartz Figure 89 Prep Flow Cell - Quartz Parts Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 244: Nano Flow Cells

    5022-2184 Union ZDV G1315-45003 Torque adapter G1315-84902 Handle for Clamp unit G1315-84910 Clamp unit 0515-1056 Screw M 2.5, 4 mm lg for cell body/clamp 8710-1534 Wrench, 4 mm both ends, open end Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 245 Parts for Maintenance Nano Flow Cells Figure 90 Content of kits Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 246 Outlet capillary, 120 mm long, 50 µm i.d. with pre-fixed ferrules (#4) and fitting (#3), plus one PEEK Fitting FT (#5) G1315-27703 Cell Housing (500 nL) G1315-87101 Cell Seal Assembly (500 nL) G1315-80001 Quartz Body (500 nL) G1315-68715 Sealing Kit Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 247 Outlet capillary, 600 mm long, 25 µm i.d. with pre-fixed ferrules (#4) and fitting (#3), plus one PEEK Fitting FT (#5) G1315-27704 Cell Housing (80 nL) G1315-42301 Cell Seal Assembly (80 nL) G1315-80002 Quartz Body (80 nL) G1315-68725 Sealing Kit 80 nL cell Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 248: High Pressure Flow Cell

    Capillary column – detector 380 mm lg, 0.17 i.d., (includes ferrule front 1/16", ferrule back 1/16" and fitting 1/16"). 79883-68700 High pressure cell repair kit (includes 1 quartz window, 1 compression washer, 5 spring washers, 2 seal rings) Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 249 Parts for Maintenance High Pressure Flow Cell Figure 91 High pressure flow cell - parts Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 250: Accessory Kits

    Capillary SST column — detector 150 mm lg, 0.17 mm i.d. 5181-1516 CAN cable, Agilent module to module, 0.5 m Items 4, 5 and 6 are included in kit 5062-2418 1/16" Fittings and Ferrules (front/back) 10/PK. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 251 Parts for Maintenance Accessory Kits Figure 92 Waste Tubing Parts Figure 93 Inlet Capillary (Column-Detector) Parts Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 252 Parts for Maintenance Accessory Kits Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 253: Identifying Cables

    Agilent 1260 Infinity DAD and MWD User Manual Identifying Cables Cable Overview Analog Cables Remote Cables BCD Cables CAN/LAN Cables Agilent 1200 module to PC This chapter provides information on cables used with the 1200 series of HPLC modules. Agilent Technologies...

  • Page 254: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent module to 3394/6 integrators...
  • Page 255 It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 256: Analog Cables

    The other end depends on the instrument to which connection is being made. Agilent Module to 3394/6 Integrators p/n 35900-60750 Pin 3394/6 Pin Agilent Signal Name module Not connected Shield Analog - Center Analog + Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 257 Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin 3394/6 Pin Agilent Signal Name module Not connected Black Analog - Analog + Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 258: Remote Cables

    Identifying Cables Remote Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent Module to 3396A Integrators...
  • Page 259 6 - Yellow 6 - Yellow Power on High 7 - Red 7 - Red Ready High 8 - Green 8 - Green Stop 9 - Black 9 - Black Start request Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 260 3 - Gray Start 4 - Blue Shut down 5 - Pink Not connected 6 - Yellow Power on High 7 - Red Ready High 8 - Green Stop 9 - Black Start request Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 261: Bcd Cables

    BCD 0 Orange BCD 3 BCD 2 Brown BCD 1 Gray Digital ground Gray Gray/pink BCD 11 Red/blue BCD 10 White/green BCD 9 Brown/green BCD 8 not connected not connected + 5 V Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 262 Agilent Module to 3396 Integrators p/n 03396-60560 Pin 3396 Pin Agilent Signal Name BCD Digit module BCD 5 BCD 7 BCD 6 BCD 4 BCD0 BCD 3 BCD 2 BCD 1 Digital ground + 5 V Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 263: Can/Lan Cables

    CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 264: Agilent 1200 Module To Pc

    It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 265: Appendix

    Agilent 1260 Infinity DAD and MWD User Manual Appendix General Safety Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Radio Interference Sound Emission UV-Radiation Solvent Information Declaration of Conformity for HOX2 Filter Agilent Technologies on Internet This chapter provides addition information on safety, legal and web.

  • Page 266: General Safety Information

    Whenever it is likely that the protection has been impaired, the instrument must be made inoperative and be secured against any intended operation. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 267 When working with solvents please observe appropriate safety procedures (e.g. goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 268 C A U T I O N alerts you to situations that could cause loss of data, or damage of equipment. ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 269: The Waste Electrical And Electronic Equipment (Weee) Directive (2002/96/Ec)

    “Monitoring and Control instrumentation” product. Do not dispose off in domestic household waste To return unwanted products, contact your local Agilent office, or see www.agilent.com for more information. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 270: Radio Interference

    If test and measurement equipment is operated with equipment unscreened cables and/or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 271: Sound Emission

    This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 272: Uv-Radiation

    Typically the radiation values are much smaller than these limits: Table 39 UV-Radiation Typical Values Position Effective Irradiance Lamp installed, 50 cm distance Average 0.016 µW/cm2 Lamp installed, 50 cm distance Maximum 0.14 µW/cm2 Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 273: Solvent Information

    Halogenated solvents or mixtures which form radicals and/or acids, for example: → 2COCl 2CHCl + 2HCl This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol, Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 274 THF, dioxane, di-isopropylether) such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides, • Solvents containing strong complexing agents (e.g. EDTA), • Mixtures of carbon tetrachloride with 2-propanol or THF. Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 275: Declaration Of Conformity For Hox2 Filter

    Appendix Declaration of Conformity for HOX2 Filter Declaration of Conformity for HOX2 Filter Agilent 1260 Infinity DAD and MWD User Manual...

  • Page 276: Agilent Technologies On Internet

    For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select Products/Chemical Analysis It will provide also the latest firmware of the modules for download. Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 277 (ChemStation only) remote reference RS-232 declaration of conformity automatic configuration with Bootp cables defect on arrival analog delivery checklist detection compound classes cable Diagnostic software Beer-Lambert (law) overview dimensions remote bench space diode Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 278 TCP/IP parameter configuration shut-down test using default synchronization lost humidity using stored timeout what you have to do first uv lamp current leak sensor open Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 279 (response time) uv lamp voltage no run data available in performance visible lamp current run recovery Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 280 (ChemStation only) GLP features holmium oxide linear range intensity noise and drift (ASTM) slit noise and linearity spectral flatness (ChemStation performance only) programmable slit width test chromatogram 181, safety and maintenance Agilent 1260 Infinity DAD and MWD User Manual...
  • Page 282 In This Book This manual contains technical reference information about the Agilent 1260 Infinity diode array and multiple wavlength detectors G1315C/D and G1365C/D. The manual describes the following: • introcduction and specifications, • installation, • using and optimizing, • troubleshooting and diagnose, •...

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