Diagnostics - GE AKTA start Maintenance Manual

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3.5.2

Diagnostics

Description
Diagnostics is used to conduct a Dark current test and/or a Stray light test to assess
electrical noise or presence of stray light.
Test
Dark current test
Stray light test
Check for electrical noise
Follow the instructions below to perform a Dark current test.
Step
1
2
ÄKTA start Maintenance Manual 29060308 AD
Description
Switches off the UV LED during the test.
The test result is presented as Pass if the Signal re-
sponse is below 50 mV.
Checks the absorbance when the UV flow cell is filled
with 15% acetone.
Absorbance (Abs) should have a value > 2000 mAU.
Action
Make sure that:
The UV flow cell is assembled tightly.
The inlet and the outlet tubing, and connectors are tightened to block
any stray light from entering.
The system is not exposed to direct sunlight.
Flush and fill the UV flow cell with demineralized water using a syringe. Make
sure that there are no air bubbles in the syringe.
3 Maintenance operations
3.5 UV Monitor
3.5.2 Diagnostics
41

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