3.5.2
Diagnostics
Description
Diagnostics is used to conduct a Dark current test and/or a Stray light test to assess
electrical noise or presence of stray light.
Test
Dark current test
Stray light test
Check for electrical noise
Follow the instructions below to perform a Dark current test.
Step
1
2
ÄKTA start Maintenance Manual 29060308 AD
Description
Switches off the UV LED during the test.
•
The test result is presented as Pass if the Signal re-
•
sponse is below 50 mV.
Checks the absorbance when the UV flow cell is filled
•
with 15% acetone.
Absorbance (Abs) should have a value > 2000 mAU.
•
Action
Make sure that:
The UV flow cell is assembled tightly.
•
The inlet and the outlet tubing, and connectors are tightened to block
•
any stray light from entering.
The system is not exposed to direct sunlight.
•
Flush and fill the UV flow cell with demineralized water using a syringe. Make
sure that there are no air bubbles in the syringe.
3 Maintenance operations
3.5 UV Monitor
3.5.2 Diagnostics
41
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