GE AKTA start Maintenance Manual page 19

Part
3
4
5
6
7
8
ÄKTA start Maintenance Manual 29060308 AD
Function
Sample valve
Pump
Pressure sensor
Wash valve
Injection valve
UV
Description
A 3-port valve that allows either the buffer or the
sample to enter the flow path. The Sample valve
enables direct application of the sample onto the
column using the Pump.
A peristaltic pump, which delivers buffer or sample
to the flow path with a flow rate of up to 5 ml/min.
For cleaning procedures, the Pump can flush the
flow path at a flow rate of 10 ml/min.
The Pressure sensor reads the pressure in the flow
path and senses overpressure.
A 3-port valve that is used to divert the flow path to
waste. The Wash valve switches automatically dur-
ing the predefined cleaning procedure, Pump wash.
In a manual run, the valve can be set to the intended
position by configuring the run parameters.
A 6-port manually operated valve that is used to
transfer the sample loaded in the sample loop on to
the column.
A sample loop is connected to the appropriate ports
of the valve. The valve is switched manually to posi-
tions:
Load sample: to allow the loading of the sample
•
into the sample loop.
Inject to column: to transfer the sample from
•
the loop on to the column during a
chromatography run.
The UV Monitor continuously measures the ab-
sorbance of the liquid in the UV flow cell at a fixed
wavelength of 280 nm. The UV flow cell has a path
length of 2 mm.
2 System description
2.2 Instrument
19