Entering Settings - Agilent Technologies G1323B User Manual

10 Running a Gradient Analysis

Entering Settings

Entering Settings

To set up the gradient analysis you will set the LC system settings to default
and then modify selected settings, the other settings will remain with their
default values. You will then save these settings to a method called GRAD.
To set up the solvent gradient, you will create a timetable in the method. This
is done using the Timetable screen, accessed by selecting the Timetable button
[F2] in the Pump settings screen.
The example assumes that the Timetable is empty. If the Timetable is not
empty, use the All Lines item, accessed by pressing the Delete button.
1 Enter the Analysis screen.
2 Set the Vial Range as 1 to 1.
3 Set the Number of Injections to 1.
4 Skip the method name field
5 Select the Settings button [F1].
6 Select LC System from the menu.
7 Press Enter.
8 Select the Default button [F7] and select the Yes button to load defaults.
9 Press the Esc key to enter the Analysis screen.
10 Enter the following values in the Analysis screen.
Table 5
Setting
Flow
Detection wavelength
Injection volume
Stoptime
11 Select the Settings button [F1].
12 Select BIN or QUAT pump from the menu.
126
Values in the Analysis Screen
Value
1.5 ml/minute
254 nm
1 µl
5 min
1200 Series Handheld Control Module Users's Guide