Preparing The Lc System - Agilent Technologies G1323B User Manual

Preparing the LC System

1 For the isocratic pump, fill the solvent bottle with the mixture of LC-grade
2 Turn on the detector lamp and pump using the On/Off button [F7] in the
3 For the quaternary pump, turn on the degasser by pressing the line-power
4 Purge the pump. For more information see
5 Allow the detector at least 15 minutes to provide a stable baseline.
6 Fill the contents of a Agilent Technologies isocratic standard sample
7 Pump the water/acetonitrile (35/65 %) mobile phase through the column for
1200 Series Handheld Control Module Users's Guide
bidistilled water (35 %) and acetonitrile (65 %). For the binary or
quaternary pump, fill one solvent bottle with bidistilled water (channel A)
and the other with acetonitrile (channel B).
Analysis screen. (Use Action keys then to select the module)
switch.
ampoule into a vial and seal the vial with a cap. Place the vial in position 1
of the autosampler tray.
10 minutes at a flow rate of 2 ml/min.
Running an Isocratic Analysis

Preparing the LC System

Chapter 3, "Using the Pump".
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