Entering Settings - Agilent Technologies G1323B User Manual

9 Running an Isocratic Analysis

Entering Settings

Entering Settings

To set up the isocratic analysis you will set the LC system settings to default
and then modify selected settings, the other settings will remain with their
default values. You will then save these settings to a method called ISO.
1 Enter the Analysis screen.
2 Set the vial range as 1 to 1.
3 Set the number of injections to 1.
4 Press Enter to skip the method name section.
5 Select the Settings button [F1].
6 Select LC System from the menu.
7 Select the Default button [F7] and select the Yes button to load defaults.
8 Press Esc.
9 Select the Settings button [F1].
10 Select the pump from the menu (either ISO, BIN or QUAT).
11 Press the Pressure button [F3].
12 Enter 400 as the upper pressure limit.
13 Select the Done button [F6].
14 Enter the following values: %B 65, (%C OFF, %D OFF for quarternary pump.
15 Set the Flow to 1,5 ml/min.
16 Confirm by selecting "Done" to enter the Analysis screen
17 Enter the values shown in
Table 4
Setting
Detection wavelength
Injection volume
Stoptime
116
If you have a binary pump, set %B to 65.).
Values in Analysis Screen
in the Analysis screen.
Table 4
Value
254 nm
1 µl
6 min
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