Agilent Technologies G1323B User Manual page 130

10 Running a Gradient Analysis
Observing the Chromatogram
9 Press the Plot button [F6] to show the chromatogram.
A typical gradient chromatogram for this analysis is shown in
mAU
Figure 17
The exact profile of the chromatogram will depend on the column you have
used. Differences in retention times and areas of the peaks in your
chromatogram and the one shown in
in the concentration of the sample from batch to batch, the quality of the
solvents used and the column temperature.
If you compare this chromatogram with the one from
Isocratic Analysis" you will notice the solvent gradient has reduced the elution
time of the fourth peak in the chromatogram.
You can rescale the plot using the Rescale button [F7], or the cursor keys or you define the
N O T E
plot window within the Setup.
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Gradient Analysis of Isocratic Standard
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Figure 17 might be a result of variations
1200 Series Handheld Control Module Users's Guide
Figure 17.
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Chapter 9, "Running an