Partially Filling The Sample Loop; How Much Sample Is Actually Injected - Agilent Technologies G1323B User Manual

6 Using the Manual Injection Valve

Partially Filling the Sample Loop

Partially Filling the Sample Loop

Use this method when only small quantities of sample are available. In this
method the syringe determines the injection volume.
1 With valve in INJECT position, use needle port cleaner to flush needle port
2 Move valve to LOAD position.
3 Fill syringe with required volume of sample (not more than half of loop
4 Insert needle of syringe into needle port until needle touches stator face.
5 Slowly inject sample.
6 Leave syringe in position and move valve to INJECT position.
7 Remove syringe.

How Much Sample Is Actually Injected?

When the sample loop is completely filled, the amount of sample injected is
equal to the volume of the sample loop plus the volume of the valve passages
(1 in rotor and 2 in stator). This means that the actual amount of sample
injected will be different to the nominal value designated to your sample loop.
However, since both standards and samples are analyzed using the same loop,
you rarely need to know the absolute volume of the loop.
If you do need to know the actual volume of a sample loop, we recommend you
calibrate it fitted to the valve, so that you also take the valve passages into
account.
86
with about 1 ml of mobile phase (this will reduce residual contamination
from previous injection).
volume).
Do not press too hard.
1200 Series Handheld Control Module Users's Guide