Automating Analyses - Agilent Technologies G1323B User Manual

2 Working with the Control Module

Automating Analyses

Automating Analyses

You can use the Sequence screen to create completely automatic unattended
analyses from sample preparation to injection. The Sequence screen is
accessed by using the Sequence button [F4] in the Analysis or Samples screen.
Using the
you can first run a method containing an injector program to do sample
preparation followed by an analytical run to analyze a batch of samples. You
can then run a second method to analyze further samples with different
analytical conditions. A delay time can be set in the sequence line. When the
second method is loaded, it waits for a specified time before starting the
analysis, allowing the column to equilibrate to the new conditions. All
sequence events can be traced in the Sequence Logbook available through the
Logbook button [F5] in the Sequence screen.
At the end of the sequence you can specify either to load a method (e.g. to
flush the LC system to remove buffer salts to avoid crystallization or to
program a soft shut-down method) or to turn off the LC system using the End
Actions button [F1]. If both options are selected, the shut-down method will be
loaded to be available for the next user. However, it will not be executed before
the turning off.
You can set up automatic recalibrations using the Calibration Settings screen.
This screen allows you to attach calibration settings to a sequence line. The
Calibration Settings screen is accessed by selecting the Calibration button in the
Sequence screen.
You can recalibrate using one or more standards and have the flexibility of
choosing various calibration intervals and patterns. You can define within a
sequence line the frequency to recalibrate and the order of calibration vial
analysis using the Alter and Multi settings. Alter analyzes the calibration vials
alternately. Multi analyzes the calibration vial or vials in complete groups
according to the calibration interval.
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screen you can link several methods together. For example,
Sequence
1200 Series Handheld Control Module Users's Guide