Zeiss Axiovert 5 Instruction Manual page 5

ZEISS
5 Operation ....................................................................................... 86
6 Care and Maintenance....................................................................131
7 Troubleshooting .............................................................................140
Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101
5.1 Prerequisites for Commissioning and Operation.................................................. 86
5.2 Switching On the Microscope............................................................................. 86
5.3 Adjusting ........................................................................................................... 87
5.3.1 Adjusting the Position of the Eyepieces ................................................. 87
5.3.2 Adjusting the Adjustable Focus Stop ..................................................... 87
5.3.3 Using the Light Manager Function......................................................... 88
5.3.4 Activating the ECO/Permanent Mode .................................................... 89
5.3.5 Setting the Parfocal Alignment .............................................................. 89
5.4 Rotating the Condenser...................................................................................... 90
5.5 Moving the Condenser ....................................................................................... 91
5.6 Setting Up for Transmitted Light Techniques ...................................................... 92
5.6.1 Setting Up for Transmitted Light Brightfield Microscopy ........................ 92
5.6.2 Setting Up for Transmitted Light Differential Interference Contrast Mi-
croscopy ............................................................................................... 93
5.6.3 Setting Up for Transmitted Light PlasDIC Microscopy ............................ 95
5.6.4 Setting Up for Transmitted Light Polarization Contrast (Axiovert 5/7
materials) .............................................................................................. 99
5.6.5 Setting Up for Transmitted Light Polarization Contrast (Axiovert 5)........ 100
5.6.6 Setting Up for Transmitted Light Phase Contrast Microscopy................. 101
5.6.7 Setting Up for Transmitted Light iHMC .................................................. 103
5.7 Setting Up for Reflected Light Techniques .......................................................... 105
5.7.1 Setting Up for Reflected Light Brightfield Microscopy ............................ 105
5.7.2 Setting Up for Reflected Light Darkfield Microscopy .............................. 108
5.7.3 Setting Up for Reflected Light Differential Interference Contrast Mi-
croscopy ............................................................................................... 109
5.7.4 Setting Up for Reflected Light C-DIC Microscopy ................................... 110
5.7.5 Setting Up for Reflected Light TIC Microscopy ....................................... 111
5.7.6 Setting Up for Reflected Light Polarization Contrast .............................. 112
5.7.7 Setting Up for Reflected Light Fluorescence Microscopy ........................ 115
5.8 Switching Off the Microscope ............................................................................ 116
5.9 Operating the Microscope via the On Screen Display (OSD) Menu ...................... 117
5.9.1 Configure Microscope Menu ................................................................. 117
5.9.2 Live View Menu..................................................................................... 121
5.9.3 Acquisition Modes................................................................................. 125
5.9.4 Acquisition Settings Menu..................................................................... 125
5.9.5 Global Settings Menu ............................................................................ 128
6.1 Safety During Cleaning and Maintenance ........................................................... 131
6.2 Maintenance Schedule ....................................................................................... 132
6.3 Maintenance Work............................................................................................. 132
6.3.1 Cleaning an Optical Surface................................................................... 132
6.3.2 Removing Water-Soluble Contamination ............................................... 134
6.3.3 Adjusting the Travel Range of the Scanning Stage 130x85 mot P; CAN . 134
6.3.4 Exchanging the LED Modules of the Colibri 3 LED Light Source ............. 135
6.3.5 Exchanging the TL LED 10 W Light Source ............................................ 136
7.1 Resetting the Microscope to the Factory Settings ............................................... 142
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