Zeiss Axiovert 5 Instruction Manual
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Zeiss Axiovert 5 Instruction Manual

Zeiss Axiovert 5 Instruction Manual

Inverted microscope for research and routine microscopy
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Instruction Manual
ZEISS Axiovert 5, Axiovert 5/7 materials
Inverted Microscope for Research and Routine Microscopy

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  • Page 1 Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials Inverted Microscope for Research and Routine Microscopy...
  • Page 2 Effective from: 02/2024 © 2024 Without the prior written consent of ZEISS, this document or any part of it must neither be translated nor reproduced or transmitted in any form or by any means - including electronic or mechanic methods, by photocopying, recording or by any in- formation or filing system.

  • Page 3: Table Of Contents

    3.2.10 Objective Labeling................. 43 3.2.11 Condensers ................... 45 3.2.12 Stages ....................49 3.2.13 Smart Control Box ................. 52 3.2.14 6-Position Reflector Turret, Encoded for P&C Modules ......53 Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 4 Assembling the Reflected Light LED 10W Light Source ........81 4.13 Assembling the Colibri 3 LED Light Source............83 4.14 Connecting the Microscope to the Mains ............85 Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 5 Exchanging the LED Modules of the Colibri 3 LED Light Source ..... 135 6.3.5 Exchanging the TL LED 10 W Light Source ..........136 7 Troubleshooting ................140 Resetting the Microscope to the Factory Settings ..........142 Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 6 Gliding Stage Z....................167 10.7.1 Assembling the Gliding Stage Z with Stage Inserts......... 168 10.8 Assembling the Heating Stage S1 ............... 168 11 Revision History................169 Glossary..................170 Index ....................172 Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 7: About This Instruction Manual

    1 About this Instruction Manual | 1.1 Text Conventions and Link Types 1 About this Instruction Manual This Instruction Manual (further called "document") is considered to be part of the Axiovert 5/7, herein after referred to as "microscope". This document contains basic steps and safety information that must be observed during opera- tion and maintenance.

  • Page 8: Explanation Of Symbols

    "DE" for Germany, "CN" for China. Date of manufacture optionally given adja- cent to this symbol Importer Authorized representative in the European Community Swiss authorized representative Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 9: Further Applicable Documents

    Observe local and national health and safety regulations for the location of installation and during National Health the use of the microscope. and Safety Consult with your ZEISS Sales & Service Partner if these regulations are in conflict with the installa- Regulations tion requirements of the microscope. Safety Data Observe the enclosed safety data sheets.

  • Page 10: Contact

    1 About this Instruction Manual | 1.5 Contact ZEISS 1.5 Contact If you have any questions or problems, contact your local ZEISS Sales & Service Partner or one of the following addresses: Headquarters Phone: +49 1803 33 63 34 Fax:...

  • Page 11: Safety

    Thanks to its virtually unrestricted sample space, this stand permits an unlimited use of conven- tional samples. In other words, it facilitates the examination of large samples, workpieces, etc. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 12: General Safety Information

    2.2.3 EMC Information 2.2.3.1 Axiovert 5 The following EMC information is valid for the Axiovert 5 microscopes. For a definition of the in- cluded microscopes, see Intended Purpose [ 11]. The microscope is intended to be used in an industrial electromagnetic environment for non-clini- cal applications or in a home healthcare environment for clinical applications.

  • Page 13: Lifetime

    주로 가정에서 사용하는 것을 목적으로 하 며, 모든 지역에서 사용할 수 있습니다. 2.2.3.2 Axiovert 5/7 Materials The following EMC information is valid for the Axiovert 5/7 materials microscopes. For a definition of the included microscopes, see Intended Purpose [ 11].

  • Page 14: Prevention Of Hazards

    Only connect electrical systems that are authorized by ZEISS to the supplied power supply cord. § Set up and operate the microscope so that the connectors are easily accessible. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 15 The protective earth connection must not be impaired by the use of extension cables. § Always use the power cords supplied by ZEISS. When an unsuitable power cord is used, ZEISS can no longer guarantee the electrical safety and functionality of the microscope. §...

  • Page 16: Thermal Hazards

    § Keep a logbook of the known biological substances/agents used when working with the mi- croscope and show it to the ZEISS service representative before they perform any work on the microscope. Immersion Oil Ensure that no immersion oil enters the surface water or the sewage system. The immersion oil can cause skin irritation.

  • Page 17: Hazards Generated With The Operating Environment

    Incorrect handling of consumables and cleaning agents can lead to property damage or skin and Hazards eye injuries. Consumables that are not approved by ZEISS can lead to property damage. Consult your ZEISS Sales & Service Partner to learn what consumables you can order and how to handle them. Hazardous...

  • Page 18: Labels On The Microscope

    2 Safety | 2.4 Labels and Lights ZEISS 2.4.1 Labels on the Microscope Fig. 1: Position of information labels on the microscopes Pos. Symbol Description Microscope type label Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 19 Carl Zeiss Microscopy GmbH Carl-Zeiss-Promenade 10 07745 Jena, Germany Serial number label Patent label Socket label: PC/Power UDI label not applicable for Axiovert 5/7 materials microscopes Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 20 EU representative and importer label * Label is placed on the microscope packag- ing. Swiss representative and importer label * Label is placed on the microscope packag- ing. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 21: Labels On The Carrier For Transmitted-Light Illumination

    2.4.3 Labels on Microscopes with Integrated Smart Control Box Fig. 3: Position of information labels on microscopes with integrated Smart Control Box Pos. Symbol Description MAC address label Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 22: Label On The Colibri 3 Light Source

    Do not look at operating lamp. Eye injury may result. WARNING: UV emitted from this product. Avoid eye and skin exposure to unshielded product. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 23: Labels On Microscopes With Led Light Source For Reflected Light

    Use appropriate shielding. CAUTION: Possibly hazardous optical radiation emitted from this product. Do not stare at operating lamp. May be harmful to the eyes. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 24: Product And Functional Description

    ZEISS 3 Product and Functional Description The Axiovert 5/7 is an inverted microscope of compact design with a small footprint. The micro- scope provides high resolution, infinity-corrected objectives for transmitted light techniques and for reflected light techniques, depending on the microscope type.

  • Page 25: Main Components

    3.1 Main Components 3.1.1 Axiovert 5 TL and Axiovert 5 TL SCB This section shows the main components of Axiovert 5 TL and Axiovert 5 TL SCB. Fig. 6: Axiovert 5 TL and Axiovert 5 TL SCB - Main components...

  • Page 26: Axiovert 5 Tl Fl Scb

    Intermediate phototube [ 6-position encoded reflector turret Carrier transmitted light illumination 6-position encoded nosepiece with ob- with white LED 10W jectives [ Mechanical stage [ Stand Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 27: Axiovert 5 Rl Scb And Axiovert 7 Rl

    3 Product and Functional Description | 3.1 Main Components 3.1.3 Axiovert 5 RL SCB and Axiovert 7 RL This section shows the main components of Axiovert 5 RL SCB and Axiovert 7 RL. Fig. 8: Axiovert 5 RL SCB, Axiovert 7 RL - Main Components...

  • Page 28: Axiovert 5 Rl Tl Scb And Axiovert 7 Rl Tl

    3.1.4 Axiovert 5 RL TL SCB and Axiovert 7 RL TL This section shows the main components of Axiovert 5 RL TL SCB and Axiovert 7 RL TL. Fig. 9: Axiovert 5 RL TL SCB and Axiovert 7 RL TL - Main components...

  • Page 29: Controls And Functional Elements On Components

    Purpose This section shows the functional components and controls of Axiovert 5 TL and Ax- iovert 5 TL SCB. Fig. 10: Axiovert 5 TL and Axiovert 5 TL SCB - Controls and functional elements (right side) Eyepieces Binocular phototube [...
  • Page 30 3 Product and Functional Description | 3.2 Controls and Functional Elements on Components ZEISS Fig. 11: Axiovert 5 TL and Axiovert 5 TL SCB - Controls and functional elements (left side) Snap button (right and left side) Focusing drive – coarse adjustment Focusing drive –...

  • Page 31: Controls And Functional Elements Of Axiovert 5 Tl Fl Scb

    Focusing drive – fine adjustment Knurled wheel for changing the reflec- Two-position filter slider mounted on tor turret position the carrier for transmitted light illumina- tion Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 32 Focusing drive – fine adjustment Solid-state light source Colibri 3 Smart Control Box [ Release lever for adjustable focus stop Permanent/ECO mode switch Axiocam Carrying handle Light shield Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 33: Axiovert 5 Rl Scb And Axiovert 7 Rl Stands

    Purpose This section shows the functional components and controls of Axiovert 5 RL SCB and Ax- iovert 7 RL. Fig. 14: Axiovert 5 RL SCB and Axiovert 7 RL - Controls and functional elements (right side) Binocular tube Eyepieces Intermediate phototube [...
  • Page 34 3 Product and Functional Description | 3.2 Controls and Functional Elements on Components ZEISS Fig. 15: Axiovert 5 RL SCB and Axiovert 7 RL - Controls and functional elements (left side) Focusing drive – coarse adjustment Focusing drive – fine adjustment...

  • Page 35: Axiovert 5 Rl Tl Scb And Axiovert 7 Rl Tl Stands

    This section shows the functional components and controls of Axiovert 5 RL TL SCB and Ax- iovert 7 RL TL. Fig. 16: Axiovert 5 RL TL SCB and Axiovert 7 RL TL - Controls and functional elements (right side) Binocular tube...
  • Page 36 Power switch On/Off slider Joystick for scanning stage control Fig. 17: Axiovert 5 RL TL SCB and Axiovert 7 RL TL - Controls and functional elements (left side) Focusing drive – coarse adjustment Focusing drive – fine adjustment Reflected light (RL) light source...

  • Page 37: Functions Of Stand Keys And Display Elements

    Switches the parfocality alignment Axiovert 7 RL TL > 8 seconds mode on: LED blinks*** RED. (simultane- Switches the parfocality alignment ously) mode off: LED blinks*** GREEN. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 38: Tl/Rl Button

    TL illuminator lost connection to the main control board. Connectors must be checked. flashing RL illuminator lost connection to the main control board. Connectors must be checked. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 39: Binocular Tubes

    30° and 60° § field of view 23 mm Fig. 19: Binocular Ergotube 30-60°/23 Eyepiece socket Pupil distance scale Binocular section Dovetail ring mount Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 40 60N § camera port with fixed light graduation (50:50) on the left side § viewing angle 45° § field of view 23 mm Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 41 Fig. 22: Intermediate Phototube H = 50 mm, Left Port Camera port Slider with beam splitter or mirror Dovetail ring mount for tube Fixing screw Dovetail ring mount for stand Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 42: Eyepieces

    6 slots for DIC sliders Fig. 24: Nosepiece with objectives Nosepiece Objective Cover cap Slot for DIC slider Knurled ring for swivelling the nose- piece Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 43: Objective Labeling

    Immersion Medium (Oil / W/ Glyc) § Adjustable cover glass correction (Corr.) § Contrast method. See Designed for polystyrene (PS) Cover glass thickness (mm) e.g. 1.0 Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 44 Under this condition all objectives can be swivelled through the travel range on the sample stage without collisions. Ensure that the sample plane is no more than 2.5 mm above the stage plane. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 45: Condensers

    Phase contrast ○ ○ ○ ○ PlasDIC ○ ○ ○ iHMC ○ ○ ○ Pol contrast ● included ○ optionally available - not available/not possible Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 46 – sliders 10x46 mm with fixed phase stop Ph 1 [ 158] – slider 10x46 mm Ph/PlasDIC, H, Ph/PlasDIC [ 157] § sliders can be inserted from the right or left Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 47 Front lens Mounting hole for phase stops and Wheel for setting the aperture di- contrast modules aphragm Knurled wheel for adjusting the modu- lator disk position Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 48 Knurled wheel for adjusting the modu- Front lens lator disk position Mounting hole for phase stops and Wheel for setting the aperture di- contrast modules aphragm Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 49: Stages

    Specimen stage 232x230 Object guide M 130x85 mm R/L Coaxial drive; knurled knob for X adjust- Coaxial drive; knurled knob for Y adjust- ment ment Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 50 Vernier scale for display of the adjust- Coaxial drive; knurled knob for Y adjust- ment range in Y ment Coaxial drive; knurled knob for X adjust- ment Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 51 ZEN. The following features and control units are available: § joystick § trackball § electronic coaxial drive; CAN Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 52: Smart Control Box

    The Smart Control Box is used for stand-alone operation (without PC) of microscope stand func- tions with ZEISS Axiocam (202, 208, 305) via On Screen Display (OSD) and for operation with Lab- scope (Windows and Apple® iOS®). Automatic functions for camera control, image enhancement functions and for reading out encrypted microscope functions are also available via the Smart Control Box.

  • Page 53: 6-Position Reflector Turret, Encoded For P&C Modules

    This ensures that users do not have to repeatedly set light intensities for each objective/reflector combination when switching between samples that require different illumination intensities. After restarting the microscope, all stored optimal light intensity values can be restored. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 54: Eco Mode

    (constructive and destructive interference) – de- pending on their phase. As a result, these interferences create image contents with intensity dif- ferences visible to the human eye. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 55: Transmitted Light Differential Interference Contrast Microscopy

    (ideal brightfield samples), but which show deflections (as scratches, cracks, dust particles etc.) on the plane surface. All such light-scattering details appear bright in the darkfield, while the reflective plane areas remain dark. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 56: Reflected Light Dic And C-Dic Microscopy

    The reflected light TIC method (Micro-interferometry; TIC = Total Interference Contrast in the cir- cular polarized light) is used in imaging and measuring sample structures that exist in different az- imuths. Evaluation of the measured values Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 57 1.0436 20x/0.50 and 50x/0.75 1.0718 50x/0.60 1.1111 50x/0.75 and 100x/0.75 1.2038 50x/0.80 1.2500 50x/0.90 and 100x/0.90 1.3929 100x/0.95 1.5241 Tab. 1: Correction depending on aperture Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 58 Tab. 2: Calculated phase jumps for compact material and vertical incidence of light For a thickness measurement (step height), half the difference of the phase jump at the respective interface must be considered: Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 59: Operating Modes

    § Image enhancement (true color, noise reduction) § Record and save images on the USB drive § Record and save videos on the USB drive Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 60: Operation Via Labscope

    Axiovert 5 RL TL SCB Info It is not intended to either directly or indirectly generate medical diagnostic results. Do not generate medical diagnostic results when using the software. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 61 Observe live images § Record and save images via software § Advanced features in Labscope Fig. 39: Smart Control Box connected to Labscope via Ethernet Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 62 Observe live images § Record and save images via software § Advanced features in Labscope Fig. 40: Axiovert connected with Labscope software via a Wi-Fi dongle Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 63: Operation Via Zen On Pc

    § Motorized z drive (only for Axiovert 7 RL and Axiovert 7 RL TL) Fig. 41: Axiovert 5/7 connected with ZEN software via a USB Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 64: Installation

    The microscope is unpacked. Procedure 1. Remove the two included screws, which are fixed to the transportation lock. 2. Remove the three screws. 3. Remove the transportation lock. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 65: Assembling The Ergo Intermediate Piece

    6. Rotate the intermediate piece into the desired position. 7. Tighten the clamping screw. Proceed in the reverse order for removal. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 66: Assembling The Binocular Tube

    5. Rotate the binocular tube into the desired ob- servation position. 6. Tighten the clamping screw. Proceed in the reverse order for removal. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 67: Assembling Components Into The Binocular Tube

    Always close unused nosepiece openings with cover caps. Info The objectives should be installed beginning from nosepiece position in order of increasing magnification. ü Prerequisite No Aqua Stop is installed [ 153]. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 68: Assigning Objectives

    Assign the objective types via MTB for ZEN. Info For additional information and detailed descriptions, refer to further applicable documents or ask your ZEISS Sales & Service Partner. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 69: Assembling The Stage

    6. From below, fix the stage to the spacer bar with a screw in each of the bar's two mounting holes. Proceed in the reverse order for removal. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 70 3. Slide the mounting frame into the object guide until it snaps into place. 4. If required, put a mounting frame insert onto the mounting frame and fix it with the sliders. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 71: Assembling The Mechanical Stage 130X85 R/L

    2. Diagonally press the mounting frame against the springs and into the stage's opening. 3. Verify that the mounting frame is seated correctly. Proceed in the reverse order for removal. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 72: Assembling The Mechanical Stage 40X40 R/L, Reflected Light

    7. Fix the stage to the stand with a screw in the middle mounting hole. 8. Use stage insert D = 115 mm for positioning of the sample. Proceed in the reverse order for removal. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 73: Assembling The Scanning Stage 130X85 Mot P; Can

    Always screw in the transport securing pin before transporting the stage or storing it away. Proceed in the reverse order for removal. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 74: Assembling The Condenser

    4. Turn the locking ring of the quick-action locking mechanism to the right. à The quick-action locking mechanism is locked. Proceed in the reverse order for removal. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 75: Assembling The Light Shield

    Mounting tool Parts and Tools  Hex key, 1.5 mm ü Prerequisite The condenser is rotated so that the mounting hole is accessible [ 90]. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 76 9. Stick the adhesive labels specifying the contrast modules combinations to the front of the condenser. 10. Only for Phase stop PH and iHMC contrast module: align the contrast module [ 103]. Proceed in the reverse order for removal. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 77: Loading The 6-Position Reflector Turret

    4. Press the upper edges of the module's retaining elements to the turret position's upper spring clips. 5. Push the modules lower edge towards the turret's center, so the retaining elements snap in to the lower spring clips. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 78: Assigning Reflector Modules

    Take maximum care not to damage any optical or mechanical part when equipping a re- flector module. Fig. 44: Mounting the filters and the beam splitter Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 79 4. Place the filter on the reflector module's respective position. Observe the correct orienta- tion 5. Screw on the retaining ring Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 80: Exchanging The Beam Splitter Of A Reflector Module Fl P&C

    Parts and Tools  Screwdriver, 3.0 mm, ball head ü Prerequisite The reflector module is removed from the reflector insert. Procedure 1. Remove both fixation screws Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 81: Assembling The Reflected Light Led 10W Light Source

    Avoid exposure of skin to the radiation. Use suitable protective equipment/protective clothing if required. Before installing or removing the light source always make sure it is switched off. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 82 4. Insert the dovetail ring of the light source into the illumination mount. 5. Fasten the clamping screw. Proceed in the reverse order for removal. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 83: Assembling The Colibri 3 Led Light Source

    1. At the illumination mount on the rear side of the stand, remove the protective cap. 2. Loosen the clamping screw. 3. Insert the LED light source with the dovetail ring into the illumination mount. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 84 5. Connect the LED light source plug to the stand socket. 6. Tighten the fixing screws on the plug. Proceed in the reverse order for removal. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 85: Connecting The Microscope To The Mains

    1. Connect the power cable to the Power socket. 2. Connect the power cable to the power supply unit. 3. Connect the power supply unit to the mains. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 86: Operation

    ü Prerequisite The microscope is connected to the mains. Procedure 1. Set the switch to ON. à The power indicator lights up with green light. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 87: Adjusting

    The microscope is operational. ü A sample is positioned on the stage. Procedure 1. Rotate the clamping lever of the stop upwards to the stop pin. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 88: Using The Light Manager Function

    The light source blinks once. This is visible through the eyepieces and on the monitor. 4. Repeat this procedure to set light intensity values for more objective/reflector combinations. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 89: Activating The Eco/Permanent Mode

    1. Press the left Snap button and the Intensity/LM knob simultaneously for > 8 s. à The LED indicator turns to red. à The Parfocality alignment mode is activated. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 90: Rotating The Condenser

    1. Turn the locking ring of the quick-action lock- ing mechanism to the left and push it upward. à The quick-action locking mechanism is unlocked. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 91: Moving The Condenser

    Procedure 1. If required, rotate the condenser [ 90]. 2. Grab the adapter ring. 3. Carefully push the condenser backwards. 4. Insert the large sample. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 92: Setting Up For Transmitted Light Techniques

    3. Place the sample on the stage. 4. Swivel in the 10x objective on the nosepiece into the beam path. 5. Focus the sample. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 93: Setting Up For Transmitted Light Differential Interference Contrast Microscopy

    – Analyzer module DIC ACR P&C for transmitted light – Analyzer module DIC ACR P&C shift free for transmitted light ü Prerequisite The microscope is operational. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 94 4. Bring the objective for DIC into the beam path. 5. On the condenser, swivel the suitable DIC I, II or III condenser module into the light path. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 95: Setting Up For Transmitted Light Plasdic Microscopy

    DIC P&C analyzer module Pol in the reflector turret  alternatively: Three-position contrast slider with built-in analyzer fixed for contrast slider ü Prerequisite The microscope is operational. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 96 4. Place the sample on the stage. 5. Bring the PlasDIC position of the condenser slider into the light path. 6. If required, increase the illumination brightness. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 97 § Three-position contrast slider with built-in LD A-Plan 10x-63x PlasDIC module (suitable for LD A-Plan 10x to 63x objectives) ü Prerequisite The microscope is operational. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 98 3. Place the sample on the stage. 4. Rotate the condenser position with the slit di- aphragm for PlasDIC into the beam path. 5. If required, increase the illumination brightness. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 99: Setting Up For Transmitted Light Polarization Contrast (Axiovert 5/7 Materials)

    The microscope is adjusted for transmitted light brightfield microscopy [ 92]. Procedure 1. On the condenser, bring the polarizer slider D into the beam path. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 100: Setting Up For Transmitted Light Polarization Contrast (Axiovert 5)

    Analyzer fixed for contrast slider 10x29 mm assembled to three-position contrast slider 10x29 mm for PlasDIC module and analyzer  alternatively: Analyzer module Pol ACR P&C for transmitted light in the reflector turret Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 101: Setting Up For Transmitted Light Phase Contrast Microscopy

    A phase ring diaphragm matching the phase contrast objective is installed in the slider. ü The illumination is adjusted for transmitted light brightfield microscopy [ 92]. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 102 Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 103: Setting Up For Transmitted Light Ihmc

    Parts and Tools  2 x Hex key, 1.5 mm ü Prerequisite The microscope is operational. ü The iHMC module in the condenser is aligned [ 103]. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 104 . Use the hex keys. 8. Remove the auxiliary microscope. 9. Attach the condenser sticker for the iHMC magnification to the corresponding surface for the condenser position used. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 105: Setting Up For Reflected Light Techniques

    Axiovert 7 RL and Axiovert 7 RL TL: The parfocal alignment is set [ 89]. Procedure 1. If required, push the button for reflected light illumination. 2. Place the sample on the stage. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 106 6. Bring the edge of the luminous-field diaphragm into focus . Use the focusing drive. 7. Center the luminous-field diaphragm . Use the centering screws of the luminous-field di- aphragm slider. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 107 13. Adjust illumination intensity by turning the knob. Info Never use the aperture diaphragm to adjust image brightness. Use the illumination intensity control for this purpose! Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 108: Setting Up For Reflected Light Darkfield Microscopy

    3. Completely open the luminous field diaphragm. 4. Completely open the aperture diaphragm. 5. Switch off or remove neutral filters if applicable. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 109: Setting Up For Reflected Light Differential Interference Contrast Microscopy

    4. Place the sample on the stage. 5. Focus until the sample structure of interest ap- pears. 6. Use the knurled screw on the DIC slider to adjust the optimal contrast. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 110: Setting Up For Reflected Light C-Dic Microscopy

    2. Swivel the C-DIC compatible objective into the beam path. 3. Insert the C-DIC slider 6x20 into the slot under the nosepiece. 4. Place the sample on the stage. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 111: Setting Up For Reflected Light Tic Microscopy

    4. Insert the TIC slider 6x20 into the slot below the nosepiece. à Chromatic interference stripes appear in the field of view. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 112: Setting Up For Reflected Light Polarization Contrast

    The illumination is adjusted for reflected light brightfield microscopy [ 105]. Procedure 1. Bring the Pol objective into the beam path. 2. If applicable, remove the DIC slider. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 113 2. Slide the analyzer slider A 12x35 mm, 90° ro- tatable into the slot under the reflector turret. The analyzer slider may be inserted both from the left-hand and right-hand sides. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 114 3. Using the analyzer turning lever, cross the vibration directions of the analyzer and the polar- izer. 4. Place the sample on the stage. 5. Focus the sample. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 115: Setting Up For Reflected Light Fluorescence Microscopy

    1. Turn the reflector turret to the empty position for transmitted light brightfield. 2. Seek the sample detail to be examined. 3. Switch off the transmitted light illumination. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 116: Switching Off The Microscope

    Bring the phosphorescence blocking filter in the beampath. 5.8 Switching Off the Microscope Procedure 1. Set the switch to OFF. 2. Cover the microscope with the dust cover. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 117: Operating The Microscope Via The On Screen Display (Osd) Menu

    Open the Shading Correction Setup submenu [ 120]. Microscope Configure the microscope. configuration area Objective selec- Select the objective and the filter set [ 118]. tion/Filter set selection area Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 118 3. Click Save to apply the selection. 4. Repeat the procedure for other objective positions, if required. 5. Click on the Filter set selection tab. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 119 9. Repeat the procedure for other filter set positions, if required. 10. Click Apply to save the selection. 11. Click < Back to return to the live image. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 120 The Shading correction Setup submenu opens. 3. Read and observe the on-screen hints on how to perform an individual shading correction. 4. Click on the Setup button. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 121: Live View Menu

    Select the desired acquisition mode, see Acquisition Modes acquisition 125]. mode icon Snap button Snaps a single image. Depending on the selected acquisition mode different types of acquisition can be performed. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 122 5. Click on the button to save the changes. Info The Configure File Name Template button opens another template in which presettings for naming the files can be made. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 123 ü The Multi-Channel acquisition mode is active. Procedure 1. Click on the desired fluorescence channel button. à A live image is displayed on the screen. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 124 A window opens in which the file name can be entered. 8. Enter a new name for the image. 9. Click on the button to save the changes. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 125: Acquisition Modes

    127]. controls Exposure Set the exposure [ 126]. controls White balance Set the white balance [ 128]. controls ECO indicator Check the ECO mode setting. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 126 Procedure 1. At the OSD menu, navigate to the Acquisition Settings menu. 2. At the Exposure controls, click the Automatic button. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 127 4. Tap the FL/RL button, if a RL or FL light source is installed. 5. If necessary, fine-tune the Light Intensity for the RL light source using the respective slider or the input field. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 128: Global Settings Menu

    Fig. 50: Global Settings menu Wi-Fi Options Tab General Tab [ 129] Update Tab [ 130] Publisher Tab [ 130] Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 129 Set date and time. setting field Import button Import an existing configuration file. Export button Export the configuration file. Reset button Reset the system configuration. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 130 The Publisher tab displays legal information regarding the publisher as well as links to the user support forum, data protection notice, and end user license agreement. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 131: Care And Maintenance

    Make sure that no cleaning liquid or moisture gets inside the microscope and its compo- nents. In case of damage, the affected parts of the microscope must be taken out of operation. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 132: Maintenance Schedule

    Avoid touching optical surfaces with fingers.  Clean cloth Parts and Tools  Cotton swab  Optical cleaning solution (85% n-hexan and 15 vol% isopropyl alcohol (IPA)) Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 133 ZEISS 6 Care and Maintenance | 6.3 Maintenance Work  Lint-free cloth Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 134: Removing Water-Soluble Contamination

    1. At the stage's bottom side, loosen one of the stop screws 2. Guide the screw to the desired position and fasten it again. 3. Repeat the former steps for the second stop screw Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 135: Exchanging The Led Modules Of The Colibri 3 Led Light Source

    For more information about the usability of LED modules for Colibri 3, see Usability of LED Mod- ules for the Colibri 3 LED Light Source [ 148]. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 136: Exchanging The Tl Led 10 W Light Source

    Axiovert 5 TL § Axiovert 5 TL SCB § Axiovert 5 TL FL SCB § Axiovert 5 RL TL SCB § Axiovert 7 RL TL Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 137 1. Remove four screws on the rear cover. 2. Remove the cover. 3. Unplug the connector of the TL light source. 4. Remove four white covers of the screws. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 138 8. Insert the slider into the new TL light source. 9. Guide the plug of the new TL light source through the hole in the carrier for transmitted light. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 139 10. Fix the TL light source with four screws and re- place the white covers. 11. Plug in the connector of the TL light source. 12. Fix the rear cover. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 140: Troubleshooting

    7 Troubleshooting | ZEISS 7 Troubleshooting The following table provides hints for solving common problems. If you cannot solve the problem or if you are unsure about a certain technical difficulty, contact your local ZEISS service represen- tative. Symptom Cause...
  • Page 141 Clean the optical surfaces of the of objectives, eyepieces, con- soiled components, see Clean- densers, filters or samples. ing an Optical Surface [ 132]. Tab. 3: Troubleshooting on the microscope Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 142: Resetting The Microscope To The Factory Settings

    After 20 s the indicator light blinks green. When the indicator stops blinking and remains GREEN, the reset to the default factory setting is successful. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 143: Decommissioning And Disposal

    Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 144: Disposal

    In general, a well-visible warning sign must be affixed to the article itself and to the outside of the packaging, together with detailed information on the type of contamination. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 145: Technical Data And Conformity

    L/N/PE 100 to 240 VAC ± 10 % connection Nominal frequency 50/60 Hz Max. current 1.5 A Rating for microscope stand 24 VDC, 5 A Protection class IP20 (IEC 60529) Overvoltage Category Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 146: Applicable Standards And Regulations

    Member States relating to the making avail- able on the market of electrical equipment designed for use within certain voltage limits Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 147 Part 2-6: Particular requirements - In vitro di- agnostic (IVD) medical equipment Declaration of Microscopes and accessories of the Carl Zeiss Suzhou Co., Ltd., are in compliance with the China China RoHS 2 RoHS Restriction of the Use of Certain Hazardous Substances in Electrical and Electronic Equip- 关于电器电子产...

  • Page 148: Usability Of Led Modules For The Colibri 3 Led Light Source

    LED module 505 nm (423052-9562-000) LED module 565 nm (423052-9602-000) LED module 590 nm (423052-9543-000) LED module 625 nm (423052-9522-000) O = usable X = not usable Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 149: Accessories And Optional System Expansions

    Only the following accessories may be used with the microscope as their safe use has been con- firmed by ZEISS. Only original parts from ZEISS may be used. Check in advance whether your mi- croscope can be retrofitted with a system expansion or accessories.

  • Page 150: Approved Optical Configurations

    ü Prerequisite The microscope is switched off and disconnected from the mains. Procedure 1. Remove four screws on the rear cover. 2. Remove the cover. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 151 8. Fix the adapter for expansion with the lower part of the two-piece screws. 9. Put the cable through the hole in the carrier for transmitted light illumination. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 152 10. Fix the carrier transmitted light illumination with the upper part of the two-piece screws. 11. Plug in the connector of the TL light source. 12. Fix the rear cover. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 153: Aqua Stop Ii

    7. Pull a large lens hood over each objective of a front diameter of 27.5 to 34 8. Close the unused nosepiece openings with cover caps Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 154: Sliders For Reflected Light And Fluorescence

    Because of an already mounted metal plate the filter slider A is also usable as manual shutters for epi-fluorescence. Position The filter slider A is inserted into the 14x40 mm slot marked F on the stand. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 155: Stop Slider A 14X40 Mm With Aperture Diaphragm

    The stop slider A is inserted into the slot marked A on the stand. Fig. 56: Stop slider A 14x40 mm with aperture diaphragm Aperture diaphragm Setting wheel Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 156: Slider 14X40 Fl Attenuator

    The stop slider A is inserted into the slot marked F on the stand. Fig. 58: Stop slider A with aperture/luminous field diaphragm Aperture/luminous field diaphragm Setting wheel Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 157: Sliders For Transmitted Light

    The slider is inserted into the slot on the LD condenser. Fig. 60: Slider 10x46 mm Ph/PlasDIC, H, Ph/PlasDIC Centering screws Empty position Neutral density filter 0.06 Empty position Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 158: Slider 10X46 Mm With Fixed Phase Stop Ph1

    The slider is inserted into the slot on the LD Condenser 0.4 for H Ph PlasDIC DIC iHMC [ 47]. Fig. 62: Polarizer slider D 10x46 mm, 90° rotatable Empty position Polarizer Lever for adjusting the vibration direc- tion of the polarizer Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 159: Position Contrast Slider 10X29 Mm

    2. Remove the cover plate. 3. Insert the PlasDIC module LD A-Plan 10x-63x into the 3 position contrast slider. 4. Fix the PlasDIC module with two screws. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 160: External Light Sources

    The HAL 100 is installed on the reflected light port on the backside of the stand. Fig. 64: HAL 100 Lamp housing Dovetail ring Vertical adjusting screw Horizontal adjusting screw Release button Adjusting screw Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 161 Remove the bulb replacement tool from the HAL 100 housing before installing the light source. Do not operate the light source with the bulb replacement tool attached to its housing. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 162 7. Attach halogen lamp to port. 8. Tighten clamping screw. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 163 Let the light source cool down before touching it. The light source does not have to be removed from the microscope for replacing the bulb. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 164: Hbo 50 Light Source

    1. At the illumination mount on the rear side of the stand, loosen the clamping screw. 2. Remove the protective cap. 3. Insert the light source's dovetail ring into the illumination mount. 4. Fasten the clamping screw. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 165 à The light arc and the mirror image overlap by one third. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 166: X-Cite Xylis® Light Source

    2. Connect the plug-in power supply to the mains power supply. 3. Refer to the instruction manual of the HXP 120 V for further installation steps. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 167: Gliding Stage Z

    § rotate the second layer of the stage If the screws are fixed, the rotation wheel cannot move. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 168: Assembling The Gliding Stage Z With Stage Inserts

    Ensure that each screw passes through the hole in the relevant spacer disk. 4. Connect the plug of the stage's cable to the controller (see separate manual). Proceed in the reverse order for removal. Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 169: Revision History

    12/2022 Editorial rework § Implementation of UKCA marking § 04/2022 Implementation of revision history § Adaptation to Regulation (EU) 2017/746 (IVDR) Tab. 4: Revision History Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 170: Glossary

    (e.g. motorized xy stages, external light sources etc.) is stored in Electromagnetic Susceptibility these configurations. Furthermore, the Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 171 Radio Frequency RL (Reflected Light) Designation for microscopy techniques to image light that was reflected by the ob- ject Smart Control Box Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...

  • Page 172: Index

    Decontamination LED modules for Colibri 3 55, 56, 94, 109 changing Disposal usability Light Manager parfocality ECO mode Ethernet Eyepiece 42, 67 Mains connection Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 173 Operators Safe Operating Condition Safety 11, 131 Scanning stage Shutdown Slider 3 position contrast PlasDIC 157, 158 with fixed phase stop Software Switching off Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101...
  • Page 174 Carl Zeiss Microscopy GmbH Carl-Zeiss-Promenade 10 phone: +49 1803 33 63 34 07745 Jena fax: +49 3641 64 3439 Germany info.microscopy.de@zeiss.com www.zeiss.com/microscopy...

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