Zeiss Axio Imager 2 Operating Manual
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Zeiss Axio Imager 2 Operating Manual

Zeiss Axio Imager 2 Operating Manual

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  • Page 2 Third party products are cited for information purposes only. This does not represent approval or recommendation of these products. Carl Zeiss Microscopy AG accepts no liability for the performance or use of such products. Issued by: Carl Zeiss Microscopy GmbH...

  • Page 3: Table Of Contents

    Warning labels ......................... 11 Warranty information ....................... 13 Other operating manuals ....................13 Overall view of Axio Imager 2 - coded ................14 Overall view of Axio Imager 2 - motorized ................. 15 INSTRUMENT DESCRIPTION ................... 16 Name and designated use ....................16 Instrument description and main features ................
  • Page 4 ZEISS Contents Axio Imager 2 3.20 Attaching the condenser carrier to the transmitted-light / reflected-light stage carrier ..50 3.21 Changing the fine-drive knob or the fine-drive disk on the stand ........50 3.22 Insert the filter in the double filter wheel ................51 3.22.1...
  • Page 5 4.8.7 User page ........................145 4.8.8 Display page ........................153 Capturing large overviews (tiles or MosaiX images) on Axio Imager 2 ....... 154 4.10 Relocate an event on a sample carrier ................156 4.10.1 Microscope equipment and basic settings................ 156 4.10.2...
  • Page 6 ZEISS Contents Axio Imager 2 4.12.7 Setting reflected-light brightfield ..................183 4.12.8 Setting reflected-light darkfield ..................186 4.12.9 Setting reflected-light DIC and reflected-light C-DIC ............187 4.12.10 Setting reflected-light TIC ....................189 4.12.11 Setting reflected light fluorescence .................. 192 4.12.12 Setting reflected-light polarization –...

  • Page 7: Introduction

    Notes on device safety INTRODUCTION Notes on device safety The Axio Imager 2 microscopes have been designed, produced and tested in compliance with DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101 "Safety requirements for electrical equipment for measurement, control, and laboratory use".
  • Page 8 INTRODUCTION Axio Imager 2 Notes on device safety The Axio Imager 2 microscopes and their original accessories are to be used only for the microscopy procedures described in this Operating Manual. Particular attention must be paid to the following instructions: The manufacturer cannot assume any liability for any other applications of the instrument, including the use of individual modules or components.
  • Page 9 Axio Imager 2 INTRODUCTION ZEISS Notes on device safety Operation of the devices in explosive environments is not permissible. Operation of the devices is only permitted in closed rooms. Gas discharge lamps, e.g. HBO 50; HBO 100 or XBO 75, emit ultraviolet radiation, which can cause burns to the eyes and skin.
  • Page 10 ZEISS INTRODUCTION Axio Imager 2 Notes on device safety For stands equipped with motorized focusing drives, there is a risk of fingers being crushed in the working area when the microscope stage is lowered. Do not reach into the area between the microscope stage / stage carrier / condenser −...

  • Page 11: Warning Labels

    Axio Imager 2 INTRODUCTION ZEISS Warning labels Warning labels Fig. 1 "Crushing Hazard" label warning on Fig. 2 STOP button the base of motorized stands The STOP button on the TFT display switches off the movement of the focusing drive instantaneously to prevent a collision with the stage or the specimen placed on it.
  • Page 12 ZEISS INTRODUCTION Axio Imager 2 Warning labels Fig. 4 Warning labels on the VIS-LED attachment lamp Fig. 5 Warning labels on the underside of the microLED 430000-7544-001 01/2016...

  • Page 13: Warranty Information

    With the exception of the work described in this Operating Manual, no maintenance or repair work is to be carried out on the Axio Imager 2. Repairs may only be performed by ZEISS Service or individuals specially authorized by ZEISS Service. In the event of a problem with the instrument, please contact your local ZEISS representative.

  • Page 14: Overall View Of Axio Imager 2 - Coded

    ZEISS INTRODUCTION Axio Imager 2 Overall view of Axio Imager 2 - coded Overall view of Axio Imager 2 - coded Eyepieces Binocular tube Reflected-light illuminator (HBO 100) Microscope stand, manual Transmitted-light illuminator (HAL 100) Mechanical stage Nosepiece Condenser Reflector turret Fig.

  • Page 15: Overall View Of Axio Imager 2 - Motorized

    Axio Imager 2 INTRODUCTION ZEISS Overall view of Axio Imager 2 - motorized Overall view of Axio Imager 2 - motorized Eyepieces Binocular phototube Reflected-light illuminator (HBO 100) Microscope stand, motorized Transmitted-light illuminator (HAL 100) TFT display Mechanical stage Nosepiece...

  • Page 16: Instrument Description

    They may also be used as true reflected-light microscopes or, if equipped with transmitted-light equipment, as combined reflected-light/transmitted-light microscopes. Typical biomedical applications of the Axio Imager 2 microscopes include: − Medical examinations in laboratories (research), clinics and medical practices − Science and research (colleges, universities) in the fields of medicine and biology −...
  • Page 17 ZEISS Instrument description and main features The Axio Imager 2 microscope is available in ten different stand versions. However, the scope of the equipment of these stands is variable and can be customized to the user's requirements within the range of optional microscope components.

  • Page 18: Equipment And Compatibility Table

    ZEISS INSTRUMENT DESCRIPTION Axio Imager 2 Equipment and compatibility table Equipment and compatibility table Stand Component Option / comment A2m M2m D2m Z2m coded Microscope stand motorized Coding PC readable coded Tube lens turret motorized 6 positions, coded 6 positions, motorized...
  • Page 19 Axio Imager 2 INSTRUMENT DESCRIPTION ZEISS Equipment and compatibility table Stand Component Option / comment A2m M2m D2m Z2m manual Reflected-light aperture diaphragm slider motorized manual Reflected light double filter wheel motorized manual FL Attenuator motorized manual Reflected light /...

  • Page 20: System Overview Of Axio Imager 2

    ZEISS INSTRUMENT DESCRIPTION Axio Imager 2 System overview of Axio Imager 2 System overview of Axio Imager 2 Condensers 430000-7544-001 01/2016...
  • Page 21 Axio Imager 2 INSTRUMENT DESCRIPTION ZEISS System overview of Axio Imager 2 Microscope stages 01/2016 430000-7544-001...
  • Page 22 ZEISS INSTRUMENT DESCRIPTION Axio Imager 2 System overview of Axio Imager 2 Tubes, eyepieces 430000-7544-001 01/2016...
  • Page 23 Axio Imager 2 INSTRUMENT DESCRIPTION ZEISS System overview of Axio Imager 2 Tube carrier, objective nosepiece 01/2016 430000-7544-001...
  • Page 24 ZEISS INSTRUMENT DESCRIPTION Axio Imager 2 System overview of Axio Imager 2 Modules, sliders, filters 430000-7544-001 01/2016...
  • Page 25 Axio Imager 2 INSTRUMENT DESCRIPTION ZEISS System overview of Axio Imager 2 Illumination 01/2016 430000-7544-001...
  • Page 26 ZEISS INSTRUMENT DESCRIPTION Axio Imager 2 System overview of Axio Imager 2 Documentation 430000-7544-001 01/2016...

  • Page 27: Objectives

    0.45 : Numerical aperture ∞ Infinite image distance; these objectives can only be used with ICS microscopes from Carl Zeiss. 0.17 : Can only be used with cover slip thickness D = 0.17 mm. − Can be used with cover slip thickness D = 0 or 0.17 mm.

  • Page 28: Eyepieces

    ZEISS INSTRUMENT DESCRIPTION Axio Imager 2 Eyepieces When immersion objectives are used, the air between the cover slip and the objective is replaced by a  liquid, which in most cases is immersion oil. The plastic oiler containing 20 ml of Immersol 581 F immersion oil (n = 1.518) is ideal for this purpose.
  • Page 29 Axio Imager 2 INSTRUMENT DESCRIPTION ZEISS Stage micrometers and eyepiece reticles Symbol Designation, technical data Cat. No. Universal calibration slide D=0 474029-9010-000 Without cover glass; positive version − Precision retrieval of objects − Location of the reference mark relative to the left and −...

  • Page 30: Technical Data

    Axio Imager stand, motorized with HBO 100 and TFT display ..approx. 390 mm x 721 mm x 505 mm Weight Axio Imager 2, coded / motorized (dependent on equipment) ........ approx. 18 kg to 40 kg Ambient conditions Transport (in packaging): Permissible ambient temperature ...................
  • Page 31 Axio Imager 2 INSTRUMENT DESCRIPTION ZEISS Technical data Operating data for coded Axio Imager 2, equipped with an integrated power supply or motorized Axio Imager 2 using the VP232-2 external power supply Operating environment ......................Closed room Protection class ............................I Ingress protection rating ........................
  • Page 32 ZEISS INSTRUMENT DESCRIPTION Axio Imager 2 Technical data Axio Imager 2, coded Stand with manual stage focusing Coarse drive ......................2 mm/revolution Fine drive ................. 0.2 mm/revolution; 2 µm increment Lifting range ....................... max. 25 mm Height stop ....................mechanically adjustable Achromatic-aplanatic universal condenser 0.9 H D Ph DIC with...

  • Page 33: First-Time Set-Up

    Unpacking and installing the microscope FIRST-TIME SET-UP The Axio Imager 2 microscope can be installed, modified and started up independently by the customer. On request, the microscope can also be installed or modified by the ZEISS Service department (fee charged).

  • Page 34: Installation Conditions And Notes

    It can also lead to reduced image quality and experimental errors. To reduce vibrations at the microscope installation location, ZEISS offers various passive and active damping options (Axio Imager (000000-0477-190 anti-vibration plate); small, air-damped system table (000000-1984-812), table top mat TS 150).

  • Page 35: Attaching Or Changing Binocular Tube Or Phototube

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Attaching or changing binocular tube or phototube Attaching or changing binocular tube or phototube All binocular tubes listed in the system overview (refer to Section 2.4) can be attached to the manual or the motorized stand as described below.

  • Page 36: Mounting The Tube Lens Turret

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Mounting the tube lens turret Mounting the tube lens turret • Use an AF 3 ball-headed screwdriver to loosen clamping screw (Fig. 11/3) and remove the tube (Fig. 11/1) upward. • Unscrew the four fastening screws (Fig. 11/2), remove coupler plate (Fig.

  • Page 37: Inserting The Eyepiece Reticles

    Make sure the reticles figure always faces the field stop. Eyepiece reticles may only be installed by Zeiss service technicians working in a dust-free environment.

  • Page 38: Setting The Interpupillary Distance On The Binocular Tube

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Setting the interpupillary distance on the binocular tube Setting the interpupillary distance on the binocular tube • To adjust the eyepiece distance to your individual interpupillary distance, swing the eyepiece tubes symmetrically toward or away from one another (Fig.

  • Page 39: Connecting Equipment To The Camera Port On The Binocular Phototube

    "Interface 60" (inside diameter 30 mm), however, can continue to be used. Microscope cameras (e.g. Carl Zeiss AxioCam), customary SLR cameras (Single Lens Reflex; 35 mm film or digital) or compact digital cameras may be connected to the camera port.

  • Page 40: Screwing In Objectives

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Screwing in objectives Screwing in objectives • Move the mechanical stage with stage carrier to the lower stop position. • Remove dust covers (Fig. 17/2) from the respective openings on the objective nosepiece. • Remove objectives (Fig. 17/1) from the case and screw them into the nosepiece (Fig.

  • Page 41: Installing And Removing P&C Reflector Modules

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Installing and removing P&C reflector modules 3.11 Installing and removing P&C reflector modules Normally, the reflector turret is factory-equipped with P&C (Push&Click) reflector modules if requested by the customer. However, the user can also change the equipment of these turrets.

  • Page 42: Changing The Filter Set In The Reflector Module Fl P&C

    Insert only fluorescence filters with a free aperture of ≥ 22 mm, otherwise the image may be masked. Make sure this requirement is met when using filters from other manufacturers. Filter sets or fully assembled FL P&C reflector modules can be ordered from ZEISS. • Remove reflector module FL P&C (Fig. 20/3) from the reflector turret and place it on a surface (refer to Section 3.11).

  • Page 43: Changing The Beam Splitter In The Reflector Module Fl P&C

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Changing the beam splitter in the reflector module FL P&C If it is necessary to mount filters that carry no directional mark (arrow), the following procedure is recommended: Mount the filters with the reflective dielectric layers in such a way that the reflective layer (Fig.
  • Page 44 ZEISS FIRST-TIME SET-UP Axio Imager 2 Changing the beam splitter in the reflector module FL P&C • Tip up the excitation half of the module (Fig. 23/1), which now is on top, and remove it from the retaining pins (Fig. 23/5b) on the...

  • Page 45: Attaching Or Changing The Condenser

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Attaching or changing the condenser 3.14 Attaching or changing the condenser • Use the coarse focusing drive to move the stage carrier to the upper stop position. • Turn height control (Fig. 25/1) lower condenser carrier (Fig.

  • Page 46: Replacing The Dic Prism On The Universal Condenser

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Replacing the DIC prism on the universal condenser 3.15 Replacing the DIC prism on the universal condenser Before replacing the prism on the motorized universal condenser, ensure that you open the aperture iris first with the corresponding control button to prevent damage of the iris blades.

  • Page 47: Changing The Dic Prism Of The Modulator Turret (Mot.)

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Changing the stage carrier 3.16 Changing the DIC prism of the modulator turret (mot.) • Unscrew clamping screw (Fig. 28/1) on the turret. • Pull the modulator turret forwards out of the stand and set it down.

  • Page 48: Setting Stops For The Adjustment Range Of The Stage Carrier Dovetail Guide

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Setting stops for the adjustment range of the stage carrier dovetail guide 3.18 Setting stops for the adjustment range of the stage carrier dovetail guide Lower stop screw (Fig. 30/5): • When employing the reflected-light / trans-...

  • Page 49: Setting The Dovetail Guide Of The Stage Carrier

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Setting the dovetail guide of the stage carrier … 3.19 Setting the dovetail guide of the stage carrier When stage carrier 430701-9901-000 is used, the specimen space needs to be moved for specimens of less than 13 mm, so that the specimen can be focused.

  • Page 50: Attaching The Condenser Carrier To The Transmitted-Light / Reflected-Light Stage Carrier

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Attaching the condenser carrier to the transmitted-light / reflected-light stage carrier 3.20 Attaching the condenser carrier to the transmitted-light / reflected- light stage carrier • Use the drive knob (Fig. 32/2) to adjust the guide of the condenser carrier (Fig.

  • Page 51: Insert The Filter In The Double Filter Wheel

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Insert the filter in the double filter wheel 3.22 Insert the filter in the double filter wheel 3.22.1 Double filter wheel, manual supplied neutral-density filter transmitted light or reflected light consists of: 1 x 50 % filter −...

  • Page 52: Double Filter Wheel, Motorized

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Insert the filter in the double filter wheel 3.22.2 Double filter wheel, motorized The motorized double filter wheel is equipped with the same filter set as the manual double filter wheel. To insert the filters in the two filter wheels, the motorized filter wheel 2x must be opened on the corresponding side.

  • Page 53: Axio Imager.z2 / Z2M High-Performance Focus

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Axio Imager.Z2 / Z2m High-performance Focus 3.23 Axio Imager.Z2 / Z2m High-performance Focus The high-performance focus allows precision functioning of the focusing drive of the microscope with heavy loads (weight of the object table and specimen more than 4 kg). The high-performance focus is a standard component of all Axio Imager.Z2 / Z2m.

  • Page 54: Deactivating The High-Performance Focus

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Mounting the TFT display on the motorized stand 3.23.2 Deactivating the high-performance focus • Use the Z drive to move the microscope stage into the lowest position. • Using the AF 3 ball-head screwdriver (Fig. 37/1) release the two locking pins (Fig.

  • Page 55: Attaching The Tft Display To The Docking Station

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Attaching the TFT display to the docking station 3.25 Attaching the TFT display to the docking station To connect the TFT display and the docking station, the microscope must be switched off. • To hook the docking station up to the...

  • Page 56: Installing The Focus Linear Sensor

    Loop on page 133). 3.27 Assembling the multidiscussion equipment Explanations and additional notes on the installation and operation of the multimedia discussion equipment for Axio Imager 2 can be found in the operating manual for the multi discussion equipment (425145-7144-000). 430000-7544-001 01/2016...

  • Page 57: Connecting To Power

    200 VAC to 240 VAC, 50 Hz – 60 Hz. power unit automatically adapts to the line voltage available. Fig. 41 Overall view of Axio Imager 2 - coded 3.28.2 Motorized stand • Insert the connecting plug (Fig. 42/2) of power...

  • Page 58: Hal 100 Halogen Illuminator

    ZEISS FIRST-TIME SET-UP Axio Imager 2 HAL 100 halogen illuminator 3.29 HAL 100 halogen illuminator The HAL 100 illuminator is used as the light source for transmitted-light and reflected-light procedures (except fluorescence) on the Axio Imager. The method for attaching the halogen illuminator to the reflected-light or transmitted-light socket is the same in both cases.

  • Page 59: Separate Power Supply Of The Hal 100 Halogen Illuminator For Reflected Light

    3.29.2 Separate power supply of the HAL 100 halogen illuminator for reflected light The Axio Imager 2 allows two 100 W halogen lights to be operated independently and at the same time on a microscope. For the combined use of transmitted light and reflected light (mixed light) on the Axio Imager 2, the halogen illuminator needs to be connected to a separate reflected light power supply.

  • Page 60: Adjusting The Hal 100 Halogen Illuminator

    ZEISS FIRST-TIME SET-UP Axio Imager 2 HAL 100 halogen illuminator 3.29.3 Adjusting the HAL 100 halogen illuminator Coarse adjustment • Loosen clamping screw (Fig. 43/1 or Fig. 43/8) and remove the operational halogen illuminator (Fig. 46/2) from the microscope stand.

  • Page 61: Replacing The Hal 100 Halogen Lamp

    Axio Imager 2 FIRST-TIME SET-UP ZEISS HAL 100 halogen illuminator 3.29.4 Replacing the HAL 100 halogen lamp Hot surface! You do not need to remove the lamp housing from the stand to replace the halogen lamp. Do not store the supplied replacement tool (Fig.

  • Page 62: Installing The Led Illuminator For Transmitted Light

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Installing the LED illuminator for transmitted light 3.30 Installing the LED illuminator for transmitted light The LED illuminator for transmitted light can be installed optionally on the transmitted light port on the rear of the instrument or on the bottom of the condenser carrier.
  • Page 63 Axio Imager 2 FIRST-TIME SET-UP ZEISS Installing the LED illuminator for transmitted light Installing the LED illuminator on the condenser carrier • Remove the halogen illuminator from the transmitted-light port and disconnect the illuminator plug. Cover the transmitted-light port with the cover cap.

  • Page 64: Vis-Led Attachment Lamp For Transmitted Light

    ZEISS FIRST-TIME SET-UP Axio Imager 2 VIS-LED attachment lamp for transmitted light 3.31 VIS-LED attachment lamp for transmitted light 3.31.1 The VIS-LED attachment lamp is only intended for use with transmitted light. The VIS-LED attachment lamp is only intended for transmitted light operation.

  • Page 65: Adjusting The Vis-Led Attachment Lamp

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Mounting the microLED attachment lamp for transmitted light 3.31.2 Adjusting the VIS-LED attachment lamp • Switch on the attachment lamp via the microscope. • Switch off the diffusion disk for transmitted light remove filter wheels transmitted light.

  • Page 66: Hbo 100 Illuminator

    ZEISS FIRST-TIME SET-UP Axio Imager 2 HBO 100 illuminator 3.33 HBO 100 illuminator 3.33.1 Inserting the HBO 103 W/2 mercury vapor short-arc lamp For safety reasons, the HBO 100 illuminator and the HBO 103 W/2 mercury vapor short-arc lamp are supplied in separate packages.

  • Page 67: Aligning The Hbo 100 Illuminator

    Axio Imager 2 FIRST-TIME SET-UP ZEISS HBO 100 illuminator 3.33.3 Aligning the HBO 100 illuminator The HBO 100 illuminator is available in two versions (manual and automatic alignment). The self-adjusting HBO 100 (423011-9901-000) is aligned automatically after switching on the transformer of the illuminator.

  • Page 68: Setting Up The Switching Mirror For Two Illuminators (Manual Or Motorized)

    ZEISS FIRST-TIME SET-UP Axio Imager 2 HBO 100 illuminator 3.33.4 Setting up the switching mirror for two illuminators (manual or motorized) With the aid of a switching mirror, two illuminators can be connected to the reflected-light port of the Axio Imager stand simultaneously, e.g. a halogen illuminator (HAL 100) and a fluorescence illuminator (HBO 100).

  • Page 69: Electrical Connections On The Rear Of The Microscope

    Ports for transmitted-light halogen illuminator Power connector Sync connector for camera synchronization LM set button for light manager function RS 232 port Fig. 58 Axio Imager 2, coded (rear side) 3.34.2 Motorized stand Key to Fig. 59: CAN port USB port...

  • Page 70: Mount Sample Stage

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Mount sample stage 3.35 Mount sample stage Sample stages can be bolted on or turned and centered depending on their type. The range of stage travel is 75 mm in the x direction and 50 mm in the y direction. The drives of the fixed stages are either on the right or left.

  • Page 71: Rotatable Mechanical Stage 75X50/240° R

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Mount sample stage The hole spacings of the specimen holder are shown in Fig. 62. Fig. 62 Hole spacing of the slide 3.35.2 Rotatable mechanical stage 75x50/240° R Removing the stage • Loosen screw cap (Fig. 63/3) from the spring housing (roughly three rotations).

  • Page 72: Adjusting The Stage Drive Length On The Ergonomic Drive

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Mount sample stage Centering the stage When objectives with high magnification are used, the centering can only be exact for one selected objective. All stages are factory-precentered, i.e. a set specimen detail remains in the image center when the stage is rotated.

  • Page 73: Removing And Attaching Additional Sleeves

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Mount sample stage 3.35.4 Removing and attaching additional sleeves Both drive knobs are fitted with additional sleeves (optional). These help achieve more sensitive adjustment of an object position and can be removed if faster specimen movement is more important.

  • Page 74: Changing The Specimen Holder

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Rotary stage Pol 3.35.6 Changing the specimen holder • Loosen the two knurled screws (Fig. 66/2). • Remove the specimen holder (Fig. 66/1) from the mechanical stage (Fig. 66/3) to the front. • Attach the desired specimen holder (Fig. 66/4)

  • Page 75: Attaching Rotary Stage Pol

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Rotary stage Pol 3.36.2 Attaching rotary stage Pol • Where necessary, loosen screw cap (Fig. 67/2) of the spring housing with about three rotations. • Place rotary stage Pol (Fig. 67/5) with the groove of the dovetail (stage bottom) on spring-loaded pin (Fig.

  • Page 76: Centering Objectives

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Rotary stage Pol 3.36.4 Centering objectives The 6-position objective nosepiece Pol of the Axio Imager is equipped with five centering objective mounts (without DIC slot) and one objective mount with DIC slot (non-centering). This mount is centered relative to the stage.

  • Page 77: Mechanical Stage 75X50 Mot. Can

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Mechanical stage 75x50 mot. CAN 3.37 Mechanical stage 75x50 mot. CAN To position the 75x50 mot. CAN stage, − the coaxial drive, − the trackball or − the joystick can be used. 3.37.1 Assembling and connecting 75x50 mot. CAN mechanical stage with coaxial drive On delivery, the mechanical stage has a transport lock.
  • Page 78 ZEISS FIRST-TIME SET-UP Axio Imager 2 Mechanical stage 75x50 mot. CAN • Insert the mechanical stage-coaxial drive cable with angled CAN bus connector (Fig. 70/3) into one of the two sockets on the coaxial drive, and the other plug into the socket (Fig. 71/1) of the mechanical stage.

  • Page 79: Assembling Mechanical Stage 75 X 50 Mot. Can And Connecting Joystick / Trackball

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Mechanical stage 75x50 mot. CAN 3.37.2 Assembling mechanical stage 75 x 50 mot. CAN and connecting joystick / trackball On delivery, the mechanical stage has a transport lock. This should be removed before attaching the stage, as otherwise the mechanical stage could be damaged.

  • Page 80: Mounting The Specimen Holder On The Mechanical Stage

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Mechanical stage 75x50 mot. CAN 3.37.3 Mounting the specimen holder on the mechanical stage • Mount the desired mechanical stage, see Section 3.35.6 on page 74. 3.37.4 CAN/USB converter On manual stands, CAN bus stages (432024-9903- 000 or 432026-9000-000) can be connected directly to the PC via the CAN / USB converter.

  • Page 81: Docking Station

    Axio Imager 2 FIRST-TIME SET-UP ZEISS Mechanical stage 75x50 mot. CAN 3.37.5 Docking station • The docking station can only be used in conjunction with the motorized stand. • If it is inconvenient to operate the microscope stand from the right-hand side, the functions of the touch screen TFT display (Fig.

  • Page 82: Installing Polarizer D, Fixed

    ZEISS FIRST-TIME SET-UP Axio Imager 2 Installing Polarizer D, fixed 3.38 Installing Polarizer D, fixed If the stand has a detachable stage carrier, remove it (see Section 3.17), set it aside, upside down, and install the polarizer from above. • Remove any installed polarizer or color filter carrier from the condenser carrier.

  • Page 83: Operation

    Axio Imager 2 OPERATION ZEISS Coded Axio Imager 2, coded, operation and function controls OPERATION Coded Axio Imager 2, coded, operation and function controls Fig. 76 Axio Imager 2, coded, operation and function controls 01/2016 430000-7544-001...
  • Page 84 ZEISS OPERATION Axio Imager 2 Coded Axio Imager 2, coded, operation and function controls Key to Fig. 76: Eyepieces (see page 85) Binocular tube (see page 85) Slot for analyzer slider (usable only if the camera path deflection, left, is not installed) (see page 85)
  • Page 85 Axio Imager 2 OPERATION ZEISS Coded Axio Imager 2, coded, operation and function controls Eyepieces (Fig. 76/1) − All "Br. foc" eyepiece types have a correction function to compensate for ametropia of the user's eyes (see Section 3.5). They also permit eyepiece reticles to be mounted (see Section 2.7).
  • Page 86 ZEISS OPERATION Axio Imager 2 Coded Axio Imager 2, coded, operation and function controls − To remove the filter wheel, introduce a screwdriver into the top hole, slightly cant it and pull the filter wheel out of its slot. − The positions of the two filter wheels are labeled and are set by turning the knurled wheels. Any combination of filters can be used.
  • Page 87 Axio Imager 2 OPERATION ZEISS Coded Axio Imager 2, coded, operation and function controls Condenser carrier (Fig. 76/23) − The condenser carrier (Fig. 77/6) is part of the stage carrier (Fig. 77/5). − Insert the condenser in the mount of the condenser carrier and fasten it by means of clamping screw (Fig.
  • Page 88 ZEISS OPERATION Axio Imager 2 Coded Axio Imager 2, coded, operation and function controls Condenser (Fig. 76/25) Dependent on the model, the achromatic-aplanatic universal condenser (Fig. 79/1) is equipped with: − Swivel-type front lens − Turret disk for: Brightfield - H...

  • Page 89: Axio Imager 2 Operation And Function Controls (Motorized Version)

    Axio Imager 2 OPERATION ZEISS Axio Imager 2 operation and function controls (motorized version) Light intensity button (Fig. 76/34) − Reduces the light intensity of halogen illuminator HAL; function corresponds to Fig. 76/14. Light intensity button (Fig. 76/35) − Increases the light intensity of halogen illuminator HAL; function corresponds to Fig. 76/14.
  • Page 90 ZEISS OPERATION Axio Imager 2 Axio Imager 2 operation and function controls (motorized version) Fig. 80 Axio Imager 2 operation and function controls (motorized version) 430000-7544-001 01/2016...
  • Page 91 Axio Imager 2 OPERATION ZEISS Axio Imager 2 operation and function controls (motorized version) Key to Fig. 80: Binocular phototube with motorized eyepiece shutter – Button for opening/closing the eyepiece shutter (see page 92) Camera path deflection left, with interface 60N (built into stand) - Operation via TFT display (touchscreen)
  • Page 92 ZEISS OPERATION Axio Imager 2 Axio Imager 2 operation and function controls (motorized version) Binocular phototube with motorized eyepiece shutter (Fig. 80/1) − In addition to the manually operated beam splitter, the binocular phototube with motorized eyepiece shutter 30°/25 (425506-0000-000) is equipped with a motorized eyepiece shutter which is operated via the button on the right side (alternately on / off) or via the TFT display.
  • Page 93 Axio Imager 2 OPERATION ZEISS Axio Imager 2 operation and function controls (motorized version) FL attenuator discrete mot. for reflected light (Fig. 80/5) − Use the FL attenuator, discrete, to attenuate the light in the fluorescence path when using the HBO 100.

  • Page 94: Switching The Microscope And Hal 100 Illuminator On/Off

    ZEISS OPERATION Axio Imager 2 Switching the microscope and HAL 100 illuminator on/off Button for quick-action stage lifting to OPERATING POSITION (Fig. 80/15 or 21) − When this switch is activated, the stage is moved into the last focus position which was saved.

  • Page 95: Switching The Hbo 100 On/Off

    Axio Imager 2 OPERATION ZEISS Switching the HBO 100 on/off Switching the HBO 100 on/off • The HBO 100 illuminator, which is used in place of the HAL 100 illuminator for fluorescence contrast examinations, is switched on and off using the on/off switch (Fig. 85/2) of the HBO 100 transformer.
  • Page 96 ZEISS OPERATION Axio Imager 2 Binocular phototube 30°/25 mot. with two camera ports (2TV tube mot.) You can set the light path and the eyepiece shutter via the buttons of the control panel (Fig. 86/4, Fig. 88/1) on the right side of the tube.

  • Page 97: The Light Manager

    Axio Imager 2 OPERATION ZEISS The Light Manager The Light Manager It is the function of the Light Manager temporarily to create optimum illumination settings for the various contrast methods and magnifications used and to make these settings reproducible to the user by providing the possibility to store them permanently.

  • Page 98: Light Manager Mode: Off

    ZEISS OPERATION Axio Imager 2 The Light Manager 4.6.1 Light Manager Mode: OFF If the Light Manager is (temporarily) switched off, the microscope behaves like a normal light microscope. Starting by selecting a magnification and a corresponding lamp voltage, the operator must readjust the voltage manually to achieve a comparable image brightness when setting higher or lower magnifications.

  • Page 99: Light Manager Mode: Smart

    Axio Imager 2 OPERATION ZEISS The Light Manager 4.6.3 Light Manager Mode: SMART In SMART mode, the Light Manager automatically calculates the optimum brightness for all objectives specified via the TFT display (or the MTB 2011) for a specific contrast method.

  • Page 100: Light Manager Of The Coded Microscope

    ZEISS OPERATION Axio Imager 2 The Light Manager 4.6.4 Light Manager of the coded microscope The Light Manager of the manual microscope controls the light intensity for specimen observation via the lamp voltage. The functioning depends on the Light Control used:...

  • Page 101: Light Manager Of Motorized Microscope

    Axio Imager 2 OPERATION ZEISS The Light Manager 4.6.6 Light Manager of motorized microscope The Light Manager of the motorized microscope (Axio Imager.M2, .M2m, .Z2, .Z2m) controls the light intensity for the observation of specimens in such a way that the operator obtains the same image brightness for all set magnifications.

  • Page 102: The Contrast Manager

    ZEISS OPERATION Axio Imager 2 The Contrast Manager The Contrast Manager The Contrast Manager is at the heart of the operation efficiency design employed in all motorized Axio Imagers. With its help, the various possible contrast methods are set automatically and reproducibly.

  • Page 103: Operating The Motorized Microscope Via The Touchscreen Of The Tft Display

    Operating the motorized microscope via the touchscreen of the TFT display On the motorized Axio Imager 2, the operator can operate and configure the microscope, set it up for different users and use optional functions via the TFT- display. The TFT-display has a touch-sensitive screen.

  • Page 104: Screen Layout

    ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display 4.8.1 Screen layout The control elements and information displays are contained on various, thematically organized pages. Basically, each page on the TFT display is divided into the following main areas (Fig.
  • Page 105 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display 4.8.1.3 Control area Tabs The control area is where the actual operation of controllable components takes place. The control area is divided into further sub-areas...
  • Page 106 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display Which contrast methods are available depends on the current microscope configuration. The following contrast methods are available: Abbr. Method Requirements (these components must be present and...

  • Page 107: Menu Structure

    (without administrator login, the user only has read-only access). In the second product generation, Axio Imager 2, the stand type (BioMed or Material) no longer has to be preselected in the user interface!

  • Page 108: Home Page

    ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display On the navigation button bar, the buttons of the first level appear on the very left (Fig. 93). Press the Microscope, Config, User and Display buttons to change the button assignment on the navigation button bar.
  • Page 109 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display User button: − When this button is pressed, the user selection page will appear (Fig. 95). A preconfigured user may be chosen on the user selection page.
  • Page 110 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display When the STOP button is pressed, the load travel is aborted and a pop-up window (Fig. 98) is displayed. The additional button which appears here serves to move the stage to the load position which was originally intended.

  • Page 111: Favorite Page

    Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display Make It Visible! Button (Fig. 100/3) − This button switches the microscope to a basic state: Transmitted-light lamp set to medium intensity (2.3 V),...
  • Page 112 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display 4.8.5.1 Revolver page Depending on the configuration carried out under the Settings menu item, different tabs and information will appear on the Revolver page.
  • Page 113 Reflected-light fluorescence counts here as a reflected-light method. For this reason, the second- generation Axio Imager 2 has no FL button. If motorized components installed, corresponding contrast is set at the touch of the button.
  • Page 114 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display C-DIC / TIC tab On this page, the desired method can be selected by pressing the relevant button, provided the configured objective and the analyzer installed in the reflector turret are suitable for this method.
  • Page 115 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display • First make the desired settings for Azimuth and Shift to store fixed positions. • Press the Save position button. A pop-up window (Fig. 108) with the four position buttons will open.
  • Page 116 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display 4.8.5.2 Light page Light path and intensity control functions are contained on the Light page. As many as four tabs are available here, depending...
  • Page 117 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display The light path can be controlled by pressing the buttons. Various options are available, depending on the type of component. The first light path switch in the 2TV tube is controlled in Fig.
  • Page 118 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display Eyepiece Top Port Back Port Bino- Splitter 1: − 2TV tube (Top Port/Back Port) and motorized camera path Shutter 50/50% deflection are installed at the Side Port.
  • Page 119 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display Eyepiece Top Port − Tube with Bino Shutter (eyepiece shutter) installed Bino- Shutter − Mirror for deflection to eyepieces is unknown. − Light path for Top Port is unknown.
  • Page 120 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display Working with two power supplies and two illuminators: Second-generation Axio Imagers equipped with an additional external CAN bus- enabled power supply. When such a device is detected during the switch-on operation, it will be automatically initialized.
  • Page 121 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display F/A RL tab The luminous-field diaphragm diameter and the aperture diaphragm for the reflected-light path can be set here. This function is only available if a motorized field diaphragm or aperture diaphragm is installed.
  • Page 122 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display 4.8.5.3 Automatic operation page This menu item contains two tabs: Soft Keys and − HW Settings − Soft Keys tab On this page, ten functions or courses of action...
  • Page 123 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display 4.8.5.4 XYZ page The Microscope/XYZ page contains two tabs: Position and Measure. The availability of the XYZ page depends on the microscope stage used: Motorized stages (only CAN bus −...
  • Page 124 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display For XY and Z, the two pairs of Set Zero buttons function as follows: Manual zero setting, i.e. the current position is defined as the zero point and the display set to zero.
  • Page 125 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display Measure tab This tab is only accessible if you use a motorized (CAN bus) stage. Otherwise, the Start button and a display for the Z-distance ∆Z are displayed on the...

  • Page 126: Config Page

    ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display 4.8.6 Config page The Config page can be accessed from the Home page by pressing the Config button on the navigation button bar. The motorized stage is configured in this part of the software.
  • Page 127 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display In the Configure Objective # popup window, you can choose among various options: Empty position button − Deletes the objective configuration from the current position.
  • Page 128 Fig. 130 Allocating the DIC slider Catalog button − The user must enter the Zeiss catalogue number (XXXXXX-XXXX-XXX) to select an objective. To execute the configuration procedure, press the Save button. To abort the procedure, press the Cancel button.
  • Page 129 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display Reflector tab Use the controls on this tab to configure the reflector turret. The tab shows up to ten buttons, depending on the actual number of objective mounts on the nosepiece.
  • Page 130 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display Only dyes may be selected instead of fluorescence filter sets. In this case, the user chooses the relevant dye from the subselection options listed.
  • Page 131 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display • If a lamp-switching mirror is employed in the reflected-light path, the Lamp port to be used needs to be allocated to that mirror in the last configuration step.
  • Page 132 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display Parfocality In this section so-called parfocality alignment can be carried out for the objectives configured in the objective nosepiece. The purpose of this function is to detect mechanical and optical tolerances between the objective nosepiece and objectives in the Z-axis for each position, and to compensate these reciprocally.
  • Page 133 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display If no TFT is installed (Axio Imager M2p), parfocality may also be adjusted manually. For this to be possible, the objectives must have been successfully configured with the aid of the MTB 2011 configuration program.
  • Page 134 Fig. 141 Stage lowering The motorized stands of the second generation Axio Imager 2 are equipped with a software focus limiter which can be set by users. They can be globally switched On or Off here. Once the software limit function is active, the focus delimiter can also be set and altered using the Work / Load buttons on the stand.
  • Page 135 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display Stage tab If a motorized microscope stage (equipped with a CAN bus controller) is used, this tab will be displayed. Once an objective has been configured, the stage is assigned a defined travel speed for the objective position concerned.
  • Page 136 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display The parcentricity function is activated or deactivated by means of the On and Off buttons. Stored values remain in the memory of the device after deactivation. When active, this function ensures that the same spot always appears in the center of the field of view when changing objectives.
  • Page 137 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display Splitter Up to three buttons are displayed here depending on the equipment of the camera path deflection and the tube used. The status of the ports is detected by the system during initialization (and on opening the Settings - Components page).
  • Page 138 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display Fig. 151 Select splitting ratio - Splitter decflection plane - cont. Miscellaneous tab This tab is used to configure other optional components of transmitted-light and reflected- light illumination.
  • Page 139 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display Illumination RL The light source to be employed for reflected light must be indicated here. If a switching mirror for two illuminators is used, it will appear in this configuration menu following successful initialization.
  • Page 140 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display Motorized DIC turret Modulator turret allocation for transmitted-light DIC. This is where the user selects the appropriate DIC prisms for turret positions 2, 3 and 4.
  • Page 141 Operating the motorized microscope via the touchscreen of the TFT display With the second-generation Axio Imager 2, it is possible to configure a number of positions for the motorized condenser. This is carried out in the MTB 2011 configuration program, and the configuration is then written directly into the condenser.
  • Page 142 New transmitted light and reflected light filter set combinations (neutral-density, color and neutral-density filters) are offered for second-generation Axio Imager 2 units. These filters are fully integrated into the light manager functions. For the sake of clarity, the MTB 2011 configuration program writes those filters directly into the filter wheel, without the user having to do so through the TFT.
  • Page 143 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display Oil stop tab This tab allows the user to switch the oil stop on and off. This function prevents a dry objective from being swiveled into immersion liquid by lowering the stage whenever you switch from an immersion to a dry objective.
  • Page 144 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display Miscellaneous This tab is used to calibrate the TFT display. Fig. 166 Microscope -> Config -> Extras -> Misc(ellaneous) 4.8.6.3 Info page The Info page only contains the Firmware tab This tab allows the user to identify the firmware version.

  • Page 145: User Page

    Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display 4.8.7 User page The user can access three other pages from this one: User selection − User configuration − Admin − 4.8.7.1 User selection page Fig.
  • Page 146 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display 4.8.7.2 User configuration page User-defined configurations can be created and saved on this page. This page is password- protected. Once the user password has been entered, the configuration of the user chosen on the User selection page can be changed.
  • Page 147 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display Each setting can be given an individual name. • Press the Rename button. • Enter the name and acknowledge by pressing the ENTER key on the keyboard.
  • Page 148 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display Buttons left tab The control buttons on the left-hand side of the stand can be assigned on this tab. • Click on the top left button pair.
  • Page 149 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display Buttons right tab The control buttons on the right-hand side of the stand can be assigned on this tab. • Click on the top right button pair.
  • Page 150 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display Favorites page tab On this page, the user can define one of the listed tabs as a direct link. • Use the arrow buttons to select the Favorites page.
  • Page 151 Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display Active illumination after start-up (light path) Here, the user can select the active light path when the microscope is switched on. Reflected light: −...
  • Page 152 ZEISS OPERATION Axio Imager 2 Operating the motorized microscope via the touchscreen of the TFT display User manage tab On this tab, the administrator can create, manage and preconfigure users. Upon pressing the User # button, a pop-up menu appears containing the following functions: User activation / deactivation −...

  • Page 153: Display Page

    Axio Imager 2 OPERATION ZEISS Operating the motorized microscope via the touchscreen of the TFT display 4.8.8 Display page Using the ◄► buttons on the Display page, the user can adjust the brightness of the TFT display and the light control LEDs.

  • Page 154: Capturing Large Overviews (Tiles Or Mosaix Images) On Axio Imager 2

    ZEISS OPERATION Axio Imager 2 Capturing large overviews (tiles or MosaiX images) on Axio Imager 2 Capturing large overviews (tiles or MosaiX images) on Axio Imager 2 Preparatory steps: 1. Insert a reticle in an eyepiece and mount the eyepiece (see Section 3.5 on page 36 f).
  • Page 155 Capturing large overviews (tiles or MosaiX images) on Axio Imager 2 Settings of the orientation of the camera image in the live image window and the axis orientation in software for capturing large overview images (mosaics) in the Axio Imager 2: Image...

  • Page 156: Relocate An Event On A Sample Carrier

    ZEISS OPERATION Axio Imager 2 Relocate an event on a sample carrier 4.10 Relocate an event on a sample carrier 4.10.1 Microscope equipment and basic settings The basic setting on the microscope corresponds to the preparatory steps for capturing large overview shots, see Section 4.9 on page 154.

  • Page 157: Automatic Component Recognition And User-Defined Component Equipment

    4.11 Automatic component recognition and user-defined component equipment The ZEISS-patented automatic component recognition (ACR) system allows essential and frequently changed microscope components such as reflector modules, objectives, filter wheels and condensers plus their equipment (such as filter sets, specifications, LED modules, spectral information, etc.) to be recognized when the system is switched on or when components are changed during operation of the microscope, and for the system configuration to be updated accordingly.

  • Page 158: Programming Of Rewritable Acr Reflector Modules

    Automatic component recognition and user-defined component equipment 4.11.1 Programming of rewritable ACR reflector modules ZEISS offers ACR-enabled (automated component recognition) reflector modules for the Axio Imager 2. Their equipment can be read automatically using ACR reflector turrets, eliminating the need for configuration by the user.

  • Page 159: Configuration Of User-Defined Filter Wheels

    4.11.2 Configuration of user-defined filter wheels ZEISS offers several pre-defined filter sets for the discreetly populated double filter wheels which can be assigned to the filter wheels using the MTB 2011 microscope configuration software. Currently, 3 pre- defined filter sets can be ordered from ZEISS: a.

  • Page 160: Configuration Of Motorized Condensers

    ZEISS OPERATION Axio Imager 2 Automatic component recognition and user-defined component equipment Naturally, custom combinations are also possible in addition to these predefined filter combinations. These can be specified via the User defined filter set menu item. In order to use the user-defined filter set later on the microscope in a reproducible way, the CLASSIC MODE must first be selected for the light manager in the settings page on the TFT.

  • Page 161: Illumination And Contrast Methods

    KÖHLER illumination principle. These fundamental rules of microscope adjustment are described in detail below in Section 4.12.1 (3) "Transmitted-light brightfield according to KÖHLER" for the Axio Imager 2. Instrument equipment −...
  • Page 162 Illumination and contrast methods Fig. 191 Microscope settings on the Axio Imager 2 for transmitted light - brightfield • Use both centering screws (Fig. 191/6 or Fig. 192/1) on the condenser carrier to center the luminous- field diaphragm image (Fig. 191/C). Then, open the diaphragm until its edge just disappears from the field of view (Fig.
  • Page 163 Axio Imager 2 OPERATION ZEISS Illumination and contrast methods The specimen field size and objective aperture change after every objective change. The centering may also alter slightly. Therefore, readjust the luminous-field diaphragm and aperture diaphragm to obtain optimum results. For < 10x objectives, the front lens of the universal condenser (if used) must be folded out and the aperture diaphragm fully opened.

  • Page 164: Setting The Transmitted-Light Darkfield

    ZEISS OPERATION Axio Imager 2 Illumination and contrast methods 4.12.2 Setting the transmitted-light darkfield General principle Due to their transparency, unstained biological specimens, such as bacteria or living cell cultures, are often barely visible (or not at all) in the transmitted-light brightfield. This changes significantly if such specimens are observed in the transmitted light darkfield.
  • Page 165 Axio Imager 2 OPERATION ZEISS Illumination and contrast methods • Reinsert the eyepiece in the tube. • If the height of the dark-field condenser is set correctly and sensitively, it is possible to reduce any residual brightening in the field of view, and the luminous-field diaphragm image is almost perfectly in focus.
  • Page 166 ZEISS OPERATION Axio Imager 2 Illumination and contrast methods • To swivel the brightfield front lens H into the optical path, set lever (Fig. 194/4) to position I (darkfield front lens D swiveled out). To swivel it out, set lever to position 0.

  • Page 167: Setting Transmitted-Light Phase Contrast

    Axio Imager 2 OPERATION ZEISS Illumination and contrast methods 4.12.3 Setting transmitted-light phase contrast General principle The phase contrast technique is ideal for examining thin, unstained specimens, e.g. culture cells. The human eye is unable to see phase differences (differences in refractive index and thickness) between the different cell components.
  • Page 168 ZEISS OPERATION Axio Imager 2 Illumination and contrast methods • If there is not perfect congruence (Fig. 196/A), use the two SW 1.5 screwdrivers (Fig. 195/1 and 4) to turn the two centering screws (Fig. 195/2 and 3) to recenter the bright phase stop until complete congruence with the dark phase ring is achieved (Fig.

  • Page 169: Setting Transmitted-Light Differential Interference Contrast (Dic)

    − Analyzer module D DIC P&C (424921-9901-000) in reflector turret. Only this module may be used on the Axio Imager 2 as it provides a homogeneous field of view in combination with the special condenser modules I, II and III.
  • Page 170 ZEISS OPERATION Axio Imager 2 Illumination and contrast methods The DIC method employs polarized light and therefore is disturbed by birefringent objects, such as films, in the light path between polarizer and analyzer which are occasionally used in conjunction with histological sections. The same applies to acrylic glass culture chambers, if the chamber bottom is made of plastic.
  • Page 171 Axio Imager 2 OPERATION ZEISS Illumination and contrast methods Adjusting the transmitted-light DIC with motorized four-position modulator turret for transmitted light • On the nosepiece, swivel in the objective suitable for DIC. • On the reflector turret, swivel in the analyzer module DIC ACR shiftfree for transmitted light.

  • Page 172: Setting Transmitted-Light Polarization For Orthoscopic Observation

    ZEISS OPERATION Axio Imager 2 Illumination and contrast methods 4.12.5 Setting transmitted-light polarization for orthoscopic observation Magnified viewing (e.g. of a thin section) in polarized light is called orthoscopy (Greek: orthos = straight; skopein = seeing) because illumination is by "straight" light rays which travel parallel to the microscope axis, with the aperture diaphragm almost closed.
  • Page 173 • Push the analyzer slider (Fig. 199/1) into the respective slot or swivel in the analyzer module (Fig. 199/4) on the reflector turret. The field of view appears dark due to the crossed polarizers. If you work with the analyzer slider on the Axio Imager 2, swivel the reflector turret to a blank turret position.
  • Page 174 ZEISS OPERATION Axio Imager 2 Illumination and contrast methods Setting the microscope The microscope has been prepared as described in Section 4.12.5.1 (3). • Rotate the rotary stage Pol with the specimen, e.g. a synthetic fiber, until the specimen appears as dark as possible.
  • Page 175 Axio Imager 2 OPERATION ZEISS Illumination and contrast methods Conclusions The grayish-white color appearing first in the bright position in the above example (Fig. 200/1 corresponds to a path difference of 150 nm according to the Michel-Lévy color chart (Fig. 201).
  • Page 176 ZEISS OPERATION Axio Imager 2 Illumination and contrast methods 4.12.5.3 Measuring path differences Measuring compensators are required for the accurate measurement of path differences. These compensators reset or compensate the path difference produced by the specimen to zero (1st order black).
  • Page 177 Axio Imager 2 OPERATION ZEISS Illumination and contrast methods − After inserting the compensator λ and rotating the specimen by 90° (2 click stops), the interference color remains white. It is then, however, a "higher-order white" and thus the path difference is > 5 λ.
  • Page 178 ZEISS OPERATION Axio Imager 2 Illumination and contrast methods 4.12.5.4 Transmitted-light circular polarization contrast Application Unlike standard polarization contrast, circular polarization contrast does not show any dark (extinction) positions that depend on the angle of rotation (azimuth) of the specimen relative to the polarizer or analyzer.
  • Page 179 Axio Imager 2 OPERATION ZEISS Illumination and contrast methods Fig. 202 Components for circular polarization contrast • Do not view an (anisotropic) specimen unless the above adjustment has been made. • Place the specimen to be examined onto the stage.

  • Page 180: Setting Transmitted-Light Polarization For Conoscopic Observation - Determining The Optical Character Of Crystals

    ZEISS OPERATION Axio Imager 2 Illumination and contrast methods 4.12.6 Setting transmitted-light polarization for conoscopic observation – determining the optical character of crystals For the classification (and thus identification) of crystalline matter, the examination of the interference image in the objective pupil yields more valuable information than that obtained by viewing the specimen itself.
  • Page 181 (Fig. 203/2) on the right side to move the reticle into the light path. • Move a selected crystal to the center of the Fig. 203 Axio Imager 2 with mounted reticle. phototube Pol • Swivel in the 40x, 50x or 100x objective and, if necessary, refocus the specimen using the focusing drive.
  • Page 182 ZEISS OPERATION Axio Imager 2 Illumination and contrast methods • Push the Bertrand lens slider into the light path. Focus the axial figure by means of the lever of the slider, on the tube lens turret, swivel the position with the Bertrand lens into the light path, and focus the axial figure by turning the focusing wheel.

  • Page 183: Setting Reflected-Light Brightfield

    Axio Imager 2 OPERATION ZEISS Illumination and contrast methods If the positions of the section are less favorable, i.e. the center of the cross of optically uniaxial, or the isogyre of optically biaxial specimens is outside the objective pupil, the optical character can be assessed as follows: •...
  • Page 184 ZEISS OPERATION Axio Imager 2 Illumination and contrast methods Setting reflected-light brightfield according to KÖHLER − The microscope should be set up correctly as described in Section 3. − Switch on the microscope. • Switch on the halogen lamp for reflected light using the reflected-light / transmitted-light toggle switch (Fig.
  • Page 185 Axio Imager 2 OPERATION ZEISS Illumination and contrast methods Fig. 205 Microscope settings on Axio Imager MAT in reflected-light brightfield 01/2016 430000-7544-001...

  • Page 186: Setting Reflected-Light Darkfield

    ZEISS OPERATION Axio Imager 2 Illumination and contrast methods 4.12.8 Setting reflected-light darkfield Application The reflected light darkfield technique is used to examine specimens that not only have reflective surfaces of different reflectivity (ideal brightfield objects), but also exhibit scratches, cracks, pores, i.e. flat surface deviations.

  • Page 187: Setting Reflected-Light Dic And Reflected-Light C-Dic

    Axio Imager 2 OPERATION ZEISS Illumination and contrast methods 4.12.9 Setting reflected-light DIC and reflected-light C-DIC Application The reflected-light DIC and reflected-light C-DIC technique (DIC = Differential Interference Contrast, CDIC = Differential Interference Contrast in Circularly polarized light) produces high-contrast images of phase specimens, i.e.
  • Page 188 ZEISS OPERATION Axio Imager 2 Illumination and contrast methods • Place the specimen on the stage, focus on it and rotate the control wheel (Fig. 2061) on the CDIC slider 6x20 or the modulator turret (Fig. 207/1) until the specimen detail of interest is visible at maximum contrast.

  • Page 189: Setting Reflected-Light Tic

    Axio Imager 2 OPERATION ZEISS Illumination and contrast methods 4.12.10 Setting reflected-light TIC Application The reflected-light TIC technique (microinterferometry; TIC = Total Interference Contrast in circularly polarized light) can be used to image and measure object structures available in different azimuths.
  • Page 190 ZEISS OPERATION Axio Imager 2 Illumination and contrast methods The values for a and b (see Fig. 210) are determined using the eyepiece reticle or the micrometer eyepiece. If you are working with white light (without interference filter), λ = 550 nm must be used.
  • Page 191 Axio Imager 2 OPERATION ZEISS Illumination and contrast methods Important: • If the step and the environment are of different materials, the phase jumps inherent in the material must be taken into account. The phase jump for all non-conductors is 180° and for semiconductors it only deviates slightly from 180°, i.e.

  • Page 192: Setting Reflected Light Fluorescence

    ZEISS OPERATION Axio Imager 2 Illumination and contrast methods 4.12.11 Setting reflected light fluorescence To change the transmission, use a discrete FL attenuator (423616-0000-000 or 423617-0000- 000). The gray filters mounted in the 2-position filter wheels (428300-9901-000 or 428301- 9901-000) are not permanently stable.
  • Page 193 Axio Imager 2 OPERATION ZEISS Illumination and contrast methods • Switch on the HBO 100 mercury vapor short-arc lamp (Fig. 211/1) and allow it to warm up to operating temperature for about 15 minutes. • On the reflector turret (Fig. 211/2), select the reflector module FL P&C containing the desired...

  • Page 194: Setting Reflected-Light Polarization - Detection Of Bireflection And Reflection Pleochroism

    ZEISS OPERATION Axio Imager 2 Illumination and contrast methods 4.12.12 Setting reflected-light polarization – Detection of bireflection and reflection pleochroism Application Reflected light polarization presents a further contrasting option for polished sections of ore minerals, coals, ceramic products, certain metals and metal alloys, as these specimens often exhibit varied reflection behavior in linearly polarized light depending on the orientation of the crystals and specimen features.
  • Page 195 Axio Imager 2 OPERATION ZEISS Illumination and contrast methods If the microscope is equipped with the phototube Pol, the following settings are required for this contrast method: The Bertrand lens must be inactive (rear push-pull rod (Fig. 212/6) on right side pulled out). The luminous-field diaphragm must be open (front push-pull rod (Fig.

  • Page 196: Care, Maintenance, Troubleshooting And Service

    ZEISS CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axio Imager 2 Instrument care CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Instrument care Care of the Axio Imager is restricted to the following operations: The Axio Imager microscopes are not equipped with any special devices for protection from substances that are corrosive, potentially infectious, toxic, radioactive, or other substances that could be hazardous to health.

  • Page 197: Instrument Maintenance

    Axio Imager 2 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE ZEISS Instrument maintenance Instrument maintenance 5.2.1 Performing checks • Makes sure the available line voltage matches the required operating voltage. • Check the power cable and plug for defects. • If any damage is visible, switch off the instrument. Only have the instrument repaired by qualified technicians.

  • Page 198: Changing Fuses On The Vp232-2 Can Power Supply

    ZEISS CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axio Imager 2 Instrument maintenance 5.2.3 Changing fuses on the VP232-2 CAN power supply Always disconnect the power plug before changing the fuses. If any fuses fail, the cause of the failure must first be determined.

  • Page 199: Troubleshooting

    ® Use of no or non-specified Use Immersol 518 F immersion immersion oil with immersion oil from Carl Zeiss; see p. 202. objectives. Air bubbles in the immersion oil. Remove the bubbles by applying new oil. 01/2016 430000-7544-001...
  • Page 200 ZEISS CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axio Imager 2 Troubleshooting Problem Cause Troubleshooting Low resolving power and poor Immersion oil on the front lens of Clean the front lens of the dry image contrast. a dry objective. objective; see p. 196.
  • Page 201 Axio Imager 2 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE ZEISS Troubleshooting Problem Cause Troubleshooting Dirt or dust in the field of view. The condenser has not been Focus the condenser and swivel focused correctly and front lens front lens 0.9 correctly in or out;...

  • Page 202: Spares, Consumables And Tools

    ZEISS CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axio Imager 2 Spares, consumables and tools Spares, consumables and tools Designation Cat. No. Designated use 12V 100W halogen lamp 380059-1660-000 For HAL 100 illuminator HBO 103 W/2 mercury vapor short-arc 380301-9350-000 For HBO 100 illuminator lamp SW 1.5 ball-headed screwdriver...

  • Page 203: Requesting Service

    Requesting service All repairs of mechanical, optical or electronic components inside the instrument and of the electrical components of the Axio Imager 2 microscopes may only be performed by ZEISS service staff or specially authorized personnel. To ensure optimum settings and trouble-free functioning of your microscope over a longer period of time, we recommend concluding a service/maintenance agreement with ZEISS.

  • Page 204: Appendix

    ZEISS APPENDIX Axio Imager 2 List of abbreviations APPENDIX List of abbreviations Alternating current Automatic component recognition Reflected light Brille / Suitable for eyeglass wearers cod. coded Canadian Standards Association Dicke / Cover slip thickness D / DF Darkfield Diameter (e.g. of filters) Differential Interference Contrast Deutsches Institut für Normung / German Standards Institute...
  • Page 205 Axio Imager 2 APPENDIX ZEISS List of abbreviations P&C Push&Click Right (drive knob to the right of the mechanical stage) Reflected light Wrench size across flats Slow-blow (fuse type) Transmitted light Television Underwriter Laboratories ultraviolet Verband Deutscher Elektrotechniker / Association for Electrical, Electronic & Information...

  • Page 206: Index

    ZEISS APPENDIX Axio Imager 2 Index Index Page 2TV tube mot............................95 3200 K ...............................86 Action ..............................73 Adjusting aid ..........................84, 85 Ambient conditions ..........................30 Ametropia ............................37 Analyzer............................ 172, 178 Analyzer module ..........................173 Analyzer slider ........................84, 85, 173 Aperture diaphragm ......................
  • Page 207 Axio Imager 2 APPENDIX ZEISS Index Differential interference contrast ....................... 169 Diffusion disk, reflected-light......................84, 89 Diffusion disk, transmitted-light ....................84, 86 Dimensions ............................30 Docking station ..........................55, 81 Ergonomic drive ..........................72 Eyecups .............................. 37 Eyepiece reticles..........................37 Eyepieces ......................
  • Page 208 ZEISS APPENDIX Axio Imager 2 Index Line voltage ............................31 LM set ............................ 84, 89, 91 Luminous-field diaphragm ..................84, 86, 91, 161, 193 Maintenance ............................. 197 Mechanical stage ......................33, 71, 74, 84 Mechanical stage 75x50 mot. CAN ......................77 Menu structure ..........................107 Mercury vapor short-arc lamp ......................
  • Page 209 Axio Imager 2 APPENDIX ZEISS Index Specimen holder ........................... 33, 74 Stage carrier ....................45, 47, 50, 70, 71, 72, 75 Stage carrier dovetail guide Adjustment range ........................... 48 Specimen space expansion ......................49 Stage focusing ............................ 32 Stage lift, quick-action ........................91, 94 Stage lowering, quick-action ......................

  • Page 210: Industrial Property Rights

    ZEISS APPENDIX Axio Imager 2 Industrial property rights Industrial property rights Instruments, instrument components or methods described in this manual are protected by patents and registered utility models: US6276804 US6392796 US5015082 CH691699 GB2306585 US6123459 DE29821694 430000-7544-001 01/2016...

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