Zeiss Axiovert 5 Instruction Manual page 44

3 Product and Functional Description | 3.2 Controls and Functional Elements on Components
3.2.10.1 LD Objectives
When working with inverted microscopes, vessels are common whose bottom thickness deviates
significantly from the usual cover glass thickness of 0.17 mm.
Normally, working distances (WD) of objectives with low magnification can bridge these distances
without any problem:
§
or
§
However, in the mid-range magnification area these working distances shrink usually to values
around or below 1 mm. Such objectives cannot be used for thicker vessel bottoms.
To remedy this deficiency, special LD objectives can be used. They have a relatively large working
distance and at the same time the usual parfocal distance of 45 mm of all other objectives.
Fig. 26: LD objective
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More detailed information on LD objectives can be found at
de/de/shop/objectives
3.2.10.2 Corr Objectives
It is important to know the exact thickness of the cover glass in order to obtain an excellent im-
age.
A correction collar of corr objectives allows to compensate different thickness of cover glasss.
To do this, select an area of the sample, and find the correction collar position which produces
the optimum focus and image contrast (refocusing will always be necessary).
Info
Risk of collision
The sample plane must be no more than 2.5 mm above the stage plane to prevent the LD corr
objective from hitting the lower part of the stage. It is possible to focus on the sample in the
vessel with the bottom thickness of 1 mm with the help of object guide and mounting frame
which fix the vessel on the stage. Under this condition all objectives can be swivelled through
the travel range on the sample stage without collisions.
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LD A-Plan 5x/0.15 M27 (FWD=11.7mm at CG=1mm polystyrene)
LD A-Plan 10x/0.25 M27 (FWD=8.5mm at CG=1mm polystyrene)
Coverglass cap
or ask your ZEISS Sales & Service Partner.
Ensure that the sample plane is no more than 2.5 mm above the stage plane.
Instruction Manual ZEISS Axiovert 5, Axiovert 5/7 materials | en-US | Rev. 6 | 431030-7011-101
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2 LD objective
https://www.micro-shop.zeiss.com/
ZEISS