Shutting Down The System; Between Analyses; Overnight Or Weekends - Waters nanoACQUITY UPLC Quick Start Manual

Shutting down the system

Caution:
Buffers left in the system can precipitate, damaging module
components.
You might want to shut down the system
•

between analyses

• overnight
• for a weekend
• for 72 hours or more
Tip:
Set system shutdown parameters in the MassLynx Shutdown Editor.
Consult the MassLynx Online Help for more information.
Between analyses
To shut down the system between analyses:
1. Between analyses, continue to pump the initial mobile phase mixture
through the column to maintain the column equilibrium necessary for
good retention-time reproducibility.
2. If a few hours will pass before the next injection, slow the flow rate in
the interim to a few tenths of a µL/min to conserve solvent.
Tip:
Ensure that Auto-Shutdown for your shutdown method is
deactivated.
3. Keep the detector operating, and the heating and trapping module at
operating temperature, during this period.

Overnight or weekends

To shut down the system overnight or over a weekend:
1. Flush the column with a 1:1 mixture of methanol/acetonitrile. This
keeps the column bed in an active, wetted state.
Requirement:
with a high-water-content mobile phase (90% water). Then stop the
pump flow.
2. Power-off the detector to lengthen lamp life.
2-24 Preparing System Hardware
If you are using buffers, you must first flush the column