Troubles In Namc Microscopy - Nikon ECLIPSE Ti2-U Instruction Manual

Chapter 5 Troubleshooting
5.1.7

Troubles in NAMC Microscopy

Problem
There is no contrast in the NAMC
image.
The contrast in the NAMC image is
poor.
NAMC image quality is poor.
Polarizer
⃞
Place the polarizer into the optical path. (☞ 3.5.1, 4.6.2)
Nosepiece / objective
⃞
Place the NAMC objective into the optical path. (☞ 3.9.1, 4.6.2)
Condenser module
⃞ Place the NAMC module into the optical path (☞ 3.4.6, 4.6.2)
Condenser
⃞
Use a NAMC condenser lens. (
Combination of optical elements
⃞ Confirm that the combination of the objective and the NAMC module is correct.
(☞ 4.6.3)
Modulator adjustment
⃞
Adjust the position of the modulator of the objective. (☞ 3.4.8)
Aperture diaphragm
⃞
Fully open the aperture diaphragm. (☞ 3.4.5)
Slit adjustment
⃞ Adjust the slit of the NAMC module according to the modulator position of the
objective. (☞ 3.4.8)
⃞
If the specimen is of a drop shape, it might have lens effect, so readjust the slit at
the desired position. (☞ 3.4.8)
Condenser module
⃞
Check if there is dust or scratches on the plastic optical system of the NAMC
module. (☞ 4.6.2)
Wiping resin optical elements with lens tissue might cause a scratch on the surface.
Wiping with solvent might harm the surface. Use a simple cleaning method, such as
with a blower.
Nosepiece / objective
⃞
Be careful not to turn the correction collar when turning the modulator ring. (☞ 3.4.8)
If the correction collar turned by mistake, adjust it again. (☞ 3.10.1)
112
Check item
☞
3.4.1, 4.6.2)