Pillar For Dia Illumination And Condenser; Stage; Nosepiece And Focusing Device; Epi-Fluorescence Attachment - Nikon ECLIPSE Ti2-U Instruction Manual

Inverted research microscope
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Chapter 5 Troubleshooting
5.2.3

Pillar for Dia Illumination and Condenser

Problem
The field diaphragm image is invisible
even though the field diaphragm is
stopped down.
The condenser does not move down.
The condenser cannot be centered.
The condenser moves down.
The polarizer rotation lever does not
turn.
The contrast shield cannot be
attached.
5.2.4

Stage

Problem
The drive range of the stage is small.
The position of the specimen is
unintentionally changed.
5.2.5

Nosepiece and Focusing Device

Problem
The image is split.
The objective contacts the specimen.
The objective does not move up.
The rotational torque of the focus
knobs is large or small.
The objective contacts the specimen or
the specimen holder during nosepiece
rotation.
5.2.6

Epi-fluorescence Attachment

Problem
When epi-illumination is turned on,
illumination does not reach the
specimen.
Switch the objective to a lower-magnification objective. (☞ 3.9.1)
Focus on the condenser. (☞ 3.4.2)
Release the condenser refocusing clamp. (☞ 3.4.2)
Check the installation position of the condenser elevating section. (☞ 7.8.2)
Check if the centering screw is missing. (☞ 3.4.3)
Correctly adjust the centering screw. (☞ 3.4.3)
Do not turn the condenser module centering screw.
Turn the condenser focus knob on both sides in the tightening direction to increase
the rotational torque. (☞ 3.4.2)
Loosen the rotational part clamp screw. (☞ 3.5.1)
Select a contrast shield (for LWD or ELWD) according to the condenser to be used.
(☞ 7.9)
Select a position suitable for the contrast shield from the two positions. (☞ 7.9)
Release the stroke limit. (☞ 7.7.4)
Secure the specimen using the stage clip or the well clamper. (☞ 3.8)
Remove the DIC slider. (☞ 3.9.2)
Set the objective refocusing lever. (☞ 3.11.3)
Release the objective refocusing lever. (☞ 3.11.3)
Adjust the rotational torque by turning the coarse-focus knob torque adjustment ring.
(☞ 3.11.2)
Move down the objective, and then turn the nosepiece. (☞ 3.9.1)
Open the FL turret shutter. (☞ 3.12.2)
114
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Eclipse t-17-369Eclipse t-17-370

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