Nikon ECLIPSE Ti2-U Instruction Manual page 118

Chapter 4 Microscopy Techniques
Objective
DF microscopy requires objectives (excluding 2x and 4x) with NA smaller than the minimum NA of the
condenser. Using an objective with a diaphragm, the diaphragm in the objective can be adjusted, enabling
DF microscopy.
TI-DF dark-field condenser adapter
Use the TI-DF dark-field condenser adapter to mount a darkfield condenser lens.
Condenser lens
Condenser lenses that allow DF microscopy are as follows:
NA
Working
distance
Microscopy
technique
Other
T-CHNA High N.A. common condenser lens unit (optional)
Use the T-CHNA High N.A. common condenser lens unit to increase the brightness during DF microscopy.
Condenser adapter provided with the T-CHNA High N.A. common condenser lens unit
The T-CHNA High N.A. common condenser lens unit is equipped with a dedicated condenser adapter.
When using a T-CHNA High N.A. common condenser lens unit, use the provided condenser adapter to
mount the condenser lens (TI-DF is unnecessary).
TI-DF dark-field condenser adapter
Darkfield Condenser Dry
Usable condenser lens
Darkfield condenser (dry type)
0.80 to 0.95
4 mm
DF
The TI-DF dark-field condenser adapter is required.
High NA lens
T-CHNA High N.A. common condenser lens unit
98
Darkfield Condenser Oil
Darkfield condenser (oil type)
1.2 to 1.43
1.5 mm
DF
Condenser adapter