Using An Objective With A Diaphragm - Nikon ECLIPSE Ti2-U Instruction Manual

Chapter 3 Usage of Components
3.10.4

Using an Objective with a Diaphragm

When performing DF microscopy, use of an objective
with diaphragm allows numerical aperture (NA)
adjustment of the objective according to the NA of
the DF condenser.
 Operation:
Check the minimum NA of the DF condenser, and
then adjust the diaphragm adjusting ring so that
the NA of the objective is smaller than the
minimum NA.
 Display:
Check the numerical aperture using the gradua-
tions on the diaphragm adjusting ring.
"Iris" mark
Example of an objective with a diaphragm
66
Diaphragm
adjusting ring