Nikon ECLIPSE Ti2-U Instruction Manual page 95

Inverted research microscope
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Adjust the position of the field diaphragm (centering adjustment)
1.
Focus on the sample in episcopic BF.
2.
Stop down the field diaphragm using the
field diaphragm open/close lever.
3.
Turn the field diaphragm centering screw
using a 2 mm hexagonal screwdriver to
move the center of the field diaphragm
image to the center of the field.
4.
Widen the field diaphragm to approximately
the same size as that of the field using the
field diaphragm open/close lever.
5.
Turn the field diaphragm centering screw to
move the center of the field diaphragm
image to the center of the field again.
Differences by diaphragm shape
There are three types of field diaphragm sliders:
Each type differs in diaphragm shape and has the
following characteristics:
• Circular field stop slider (TI2-F-FSC)
Slider with a circular field diaphragm
• Square field stop slider (TI2-F-FSS)
Slider with a square field diaphragm This type is
suitable for microscopy with a camera having a
square imaging element.
• Rectangle field stop slider (TI2-F-FSR)
Slider with a rectangular (3:2) field diaphragm This
type is suitable for microscopy with a camera
having a horizontally long imaging element.
Field diaphragm image
Eyepiece field ring
Centering and size adjustment for the field diaphragm
Circle
Difference by the shape of the field diaphragm
75
Chapter 3 Usage of Components
Square
Rectangle

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