Nikon ECLIPSE Ti2-U Instruction Manual page 43

2.4 Episcopic Fluorescence (Epi-FL) Microscopy (Continued)
3
Adjust the epi field diaphragm.
3-1 Turn the epi field diaphragm knob until the
field diaphragm image becomes visible in the
field of view.
[k] Field diaphragm open/close lever (
▼
3-2 Move the epi field diaphragm image to the
center of the field of view.
[k] Field diaphragm centering screw (
▼
3-3 Adjust the size of the epi field diaphragm
image to closely match the size of the field of
view.
[k] Field diaphragm open/close lever (
▼
3-4 For a fine adjustment, change the objective to
a higher magnification objective and repeat
the procedure from step 3-1 to step 3-3.
4
4-1 Change the objective to the objective for
☞
3.13.3)
4-2 When using a liquid immersion type
☞
3.13.3)
4-3 Adjust the size of the epi field diaphragm
☞
3.13.3)
4-4 When an objective with correction collar is
4-5 Move the stage and turn the focus knobs to
23
Perform Epi-FL microscopy.
fluorescence microscopy with a specified
magnification.
☞
[b] Nosepiece ( 3.9.1)
▼
objective, perform oil or water immersion.
☞
[f] Objective ( 3.10.2, 3.10.3)
▼
image and move it to the center of the field of
view.
[k] Field diaphragm open/close lever (
[k] Field diaphragm centering screw (
▼
used, adjust the correction collar.
☞
[f] Objective ( 3.10.1)
Adjust the correction collar of the objective according
to the thickness of the prepared specimen slide or the
cover glass of the culture vessel.
▼
observe the sample.
☞
[o] Stage handle ( 3.8.3)
☞
[p] Focus knob ( 3.11.1)
Chapter 2 Microscopy
☞
3.13.3)
☞
3.13.3)