Nikon ECLIPSE Ti2-U Instruction Manual page 125

Problem
The image quality is poor.
Dirt or dust is highly visible in the
image.
The image is dark.
The image is large.
Specimen / stage
⃞
Check the orientation of the cover glass surface of the specimen. (☞ 3.10.1)
⃞ Check if the cover glass thickness is appropriate. (☞ 3.10.1)
⃞
If uneven blurring occurs, or an out-of-focus condition occurs as a result of stage
movement, check whether the specimen is tilted.
Nosepiece / objective
⃞
Check that there is no air bubbles between the specimen and the objective. (Oil
immersion objective: ☞ 3.10.2)
⃞
Remove the DIC slider from the optical path. (☞ 3.9.2)
⃞ Correctly adjust the correction collar of the objective. (☞ 3.10.1)
⃞
Use appropriate immersion liquid. (☞ 1.2.7, 3.10.2, 3.10.3)
⃞
Clean the tip of the objective. (☞ 6.1.1)
Condenser
⃞
Adjust the height of the condenser. (☞ 3.4.2)
Filters
⃞
Remove the GIF filter if the field of view is green. (☞ 3.3.4)
⃞
If the field of view has coloring, remove the filter cube or the barrier filter from the
optical path. (☞ 3.12.1)
Environment
⃞ Check if there is ambient light (such as display screen light) entering the image.
(☞ 3.3.6)
⃞
Correctly select the optical path and eliminate the influence from the ambient light by
the split optical path. (☞ 3.2.1)
Maintenance
⃞ Clean the surface of specimens and culture vessels
⃞
Clean the objective. (☞ 6.1.1)
⃞ Clean the eyepieces. (☞ 6.1.1)
⃞
Clean the condenser. (☞ 6.1.1)
⃞
If dirt or dust moves when the camera is turned, clean the camera.
⃞
Clean the relay lens. (☞ 6.1.1)
Check the upper surface of the condenser lens and the lower surface of the
objective.
Polarizer / Analyzer
⃞
Remove the polarizer from the optical path. (☞ 3.5.1)
⃞
Pull out the analyzer slider until it clicks. (☞ 3.5.2)
⃞
Remove the analyzer block from the optical path (☞ 3.5.2)
FL turret
⃞
Remove the filter cube from the optical path. Place the required filter cube into the
optical path. (☞ 3.12.1)
Tube base unit
⃞
Correctly set the optical path changeover knob. (Eyepiece tube base unit with port:
☞ 3.7.1)
Intermediate magnification dial
⃞
Return the intermediate magnification dial to 1x. (☞ 3.2.2)
Camera
⃞
Confirm the adapter's variable magnification lens setting. (☞ 7.14)
105
Chapter 5 Troubleshooting
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