Combination Of Optical Elements - Nikon ECLIPSE Ti2-U Instruction Manual

Chapter 4 Microscopy Techniques
Light source
The fluorescence LED illumination system is used for the epi-fluorescence attachment.
For details on wavelength characteristics of the light source, see the instruction manual supplied with the
light source.
Filter cube
The fluorescent filter cube (mounted on the turret)
consists of three types of optical components: the
excitation filter (EX filter), the barrier filter (BA filter),
and the dichroic mirror (DM).
Select the filter cube with the combination of suitable
optical components according to the characteristics
of the specimen and the fluorescence dye.
4.4.3

Combination of Optical Elements

When using a side port of the microscope to perform microscopy with a large field with a field number of
equivalent to or more than 22, use the following combination of illumination attachment.
• EPI-FL module + assorted main branch
• Filter cube for large field of view
Large field-of-view microscopy
• When using a side port of the microscope to perform microscopy with a large field with a field number of
equivalent to or more than 22, remove the epi field diaphragm from the optical path.
• When the C mount is used or a stage-up kit is mounted, the maximum field number is 22 on the side
port of this microscope.
• Depending on the type of the objective, the field periphery might be out of focus.
Excitation filter
Fluorescent filter cube
Excitation filter
Filter cube for large FOV
* For this microscope, the absorption filter should face downward
when mounted inside the FL turret. The figure is shown upside
down for explanatory purposes.
96
Barrier filter
C -F L
-C
U V - 2 A
E X 33
0- 38 0
D M 40
0
B A 41
0
Dichroic
mirror (internal)
Barrier filter
Dichroic mirror
(internal)