Using The Objective; Adjusting The Objective With The Correction Collar - Nikon ECLIPSE Ti2-U Instruction Manual

Chapter 3 Usage of Components
3.10

Using the Objective

3.10.1

Adjusting the Objective with the Correction Collar

When observing a sample through the bottom plate
(cover glass) of a transparent vessel such as a petri
dish or a culture bottle, aberration caused by the
difference in thickness of the cover glass can be
corrected by using an objective with a correction
collar.
 Operation:
Correction collar
Turning the correction
collar
The current correction value can be checked by
the graduations and the index mark on the
correction collar.
Correction collar adjustment procedure
1. Match the graduation of the correction
collar with the thickness of the cover glass
of the vessel.
When measuring the thickness of the glass,
either physically measure it or refer to the
standard value used by the vessel
manufacturer.
2. Focus on the specimen with the focus
knobs.
3. If the resolution and contrast of the image
are poor, slightly turn the correction collar
on the objective in either direction.
This will shift the focus slightly. Readjust the
focus by turning the fine-focus knob.
Adjustment of the correction collar
• Nikon recommends writing down the location of the correction collar of the best resolution. That will
serve as a reference when a vessel with a different thickness of the cover glass is used.
• The position of 0 mm at the correction collar indicates the position for observation of specimens without
cover glass in an inverted microscope.
• Portions with varying thicknesses (such as periphery of the vessel) in the cover glass cannot be
corrected. Use the correction function where the thickness of the glass is uniform.
Appearance of the
image
Aberration caused by
difference in glass
thickness is corrected.
Cover glass
thickness
(Example:
∞/0.13-0.20)
Correction
collar
Graduations
Example of an objective with a correction collar
4. If the image becomes clearer, slightly turn
the correction collar again in the same
direction. If the image becomes less clear,
slightly turn the collar in the opposite
direction.
5. Adjust the focus again, and check the clarity
of the image.
6. Repeat steps 4 and 5 until the clearest
image is obtained.
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