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Wellcome Trust Centre for Human Genetics
Molecular Cytogenetics and Microscopy Core
Using the Nikon TE2000 Inverted Microscope
Colour
image acquisition using NIS-Elements
[Basic Research] software and the Nikon
Digital Sight DS-5M
Handle the microscope gently, taking care to avoid sharp impacts.
1)
TO SWITCH-ON THE MICROSCOPE AND LOOK AT YOUR SAMPLE:
Bright-field Microscopy Imaging [with the Colour Camera]:
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Turn on the transmitted light by turning on the switch on the Nikon power
supply box on the bench to the left side of the microscope.
Press "lamp on/off" button at the base of the microscope on the left.
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Adjust the light intensity by rotating the control dial situated on the left side of
the microscope, below the on/off switch.
For bright-field [non-fluorescence] transmission imaging: Check that the "D"
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(Diffuser) and "HA" (Heat Absorber) filters at the top left of the microscope are
pushed in. Select the optional neutral density ND filters as necessary to dim the
light intensity [top right]: ND16 and ND2 – push both in for maximum light
attenuation, normally the best setting.
The "eyepiece turret" dial should be set to O (open), when set to C it is closed.
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The "port/optical path" dial on the right side of the base should be set to port 1.
All the light now goes to the microscope eyepieces and none to the cameras.
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The "reticule in/out" lever at front of the base to the right should be turned
clockwise and set to a 2 o'clock position. This lever is under the colour camera.
Colour
INSTRUCTIONS
PLEASE HANDLE CAREFULLY!
Camera
1
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Summary of Contents for Nikon TE2000
- Page 1 TO SWITCH-ON THE MICROSCOPE AND LOOK AT YOUR SAMPLE: Bright-field Microscopy Imaging [with the Colour Camera]: Turn on the transmitted light by turning on the switch on the Nikon power supply box on the bench to the left side of the microscope.
- Page 2 The fluorescence filter wheel located underneath the objective wheel should be on ↔ the empty position indicated by the double arrow symbol ( Place the culture vessel or glass slide on the stage. Move a low power objective (e.g. 4x or 10x) into the light path. Focus on the specimen.
- Page 3 TO START THE NIKON COLOUR CAMERA AND CAPTURE IMAGES: Switch on the colour camera power supply by pressing the button on the box labelled Nikon Sight DS-U1. The Nikon DS-5M colour camera is on the front port of the Nikon microscope.
- Page 4 You can modify the live image by altering the DS-5M Settings options [image acquisition parameters], e.g. exposure, sharpen and contrast. Exposure settings With exposure set to Manual, adjust the exposure time as required. The time is listed in milliseconds to seconds. A slower exposure time will brighten the image. You can change the exposure mode from Manual to Auto Exposure.
- Page 5 Capturing an image freezes the ‘Live-Fast’ window, so click the green play Live(+) button to restore the live video image. If both the Nikon colour and Hamamatsu B&W cameras are switched on, you can swap between them via the main menu Acquire, Select Driver.
- Page 6 The 60x microscope oil objective should only be cleaned with the lens tissues provided. The standard Nikon air objectives don’t need cleaning after use. ___________________________________________________________ If you have any microscope/imaging problems or queries, just ask a member of the Microscopy Core staff for help.
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Page 7: Troubleshooting
Port 1 is for observation down the microscope. Port 5 is for the Hamamatsu B&W camera only. Port 4 is split 50/50 between the microscope and the Nikon colour camera. Is the light too faint or too bright? Remove/Add the neutral density filters. Ensure the black T-A polariser slider isn’t pushed in [left side, under the objectives] unless you need it.
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