Nikon ECLIPSE Ti2-U Instruction Manual page 39

2.2 Diascopic Phase Contrast (Ph) Microscopy (Continued)
3
Perform Ph microscopy.
3-1 Fully open the dia aperture diaphragm.
[g] Aperture diaphragm open/close lever (
▼
3-2 Adjust the size of the dia field diaphragm
image.
[h] Field diaphragm dial (
▼
3-3 When using an objective with a correction
collar, adjust the correction collar.
☞
[b] Objective ( 3.10.1)
Adjust the correction collar of the objective according
to the thickness of the prepared specimen slide or the
cover glass of the culture vessel.
▼
3-4 Move the stage and turn the focus knobs to
observe the sample.
☞
[i] Stage handle ( 3.8.3)
☞
[c] Focus knob ( 3.11.1)
▼
3-5 When using another objective, repeat the
procedure from step
☞
3.4.5)
☞
3.3.3)
2 .
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Chapter 2 Microscopy