Diascopic Dark-Field (Df) Microscopy - Nikon ECLIPSE Ti2-U Instruction Manual

Chapter 2 Microscopy
2.5

Diascopic Dark-field (DF) Microscopy

Configure the microscope to the basic
1
state.
1-1 Turn on the power to the system.
☞
[a] Power switch (
▼
1-2 Place the 10x objective into the optical path.
☞
[b] Nosepiece ( 3.9.1)
▼
1-3 Switch the optical path of the tube base unit
to (binocular part).
[c] Tube base optical path changeover knob (☞ 3.7.1)
▼
1-4 Switch the optical path to the required
observation port.
[d] Optical path changeover dial (
▼
1-5 Configure the following controls to the basic
state.
[e] Intermediate magnification dial (1x:
[f] Objective with a correction collar (Set the correction
collar to the reference position:
▼
1-6 Remove unnecessary optical elements from
the optical path.
☞
• Polarizer ( 3.5.1)
☞
• Analyzer ( 3.5.2)
☞
• DIC slider ( 3.9.2)
☞
• Filter cube ( 3.12.1)
Configuration of the microscope
Change the condenser from the one for BF microscopy to
the one for DF microscopy. Use an objective (excluding 2x
and 4x) with smaller NA (NA 0.7 or less for dry condensers,
or NA 1.1 or less for oil condensers) than the minimum
numerical aperture of the DF condenser.
Use an objective with diaphragm if required.
[g]
[k]
[t]
[n]
[r]
[s]
[b]
[c]
[i]
[e]
[j]
[i]
Note: Letters within [ ] correspond to those in the procedure.
3.1)
☞
3.2.1)
☞
3.2.2)
☞
3.10.1)
[p]
[o]
[f]
[l]
[h]
[a]
[m]
[q]
[d]
2
Set a sample and focus on it.
2-1 Open the dia-illumination shutter, and close
the FL turret shutter.
[g] Dia-illumination shutter knob (
[h] FL turret shutter knob (
▼
2-2 Turn on the dia-illumination LED and adjust
the brightness.
[i] Dia-illumination ON/OFF button (
[j] Dia-illumination brightness adjuster (
Illumination reaches the observation port.
Use filters if required.
▼
2-3 Fully open the dia field diaphragm.
[k] Field diaphragm dial (
▼
2-4 Place the sample on the stage.
☞
[l] Stage ( 3.8.1)
When placing a large specimen on the stage, tilt the
pillar for dia-illumination backward to obtain sufficient
☞
space ( 3.3.5).
▼
2-5 Move the required part of the sample into the
center of the field of view.
[m] Stage handle (
▼
2-6 When using an oil condenser, apply a drop of
oil between the sample and the tip of the
condenser lens.
[n] DF condenser (
▼
2-7 Lower the condenser to 1 mm above the
sample.
[o] Condenser focus knob (
Be careful not to allow the tip of the condenser to collide
with the specimen. If the condenser cannot be lowered to
the required position, release the refocusing clamp.
▼
2-8 Tighten the condenser refocusing clamp.
[p] Condenser refocusing clamp (
To prevent collision with the sample, be sure to tighten the
refocusing clamp at the position after lowering the condenser.
▼
2-9 Focus on the sample.
[q] Focus knob (
▼
2-10 Adjust the diopters and the interpupillary
distance if required.
☞
[r] Eyepiece (
[s] Binocular part (
24
☞
3.3.1)
☞
3.12.2)
☞
3.3.2)
☞
☞
3.3.3)
☞
3.8.3)
☞
3.4.4)
☞
3.4.2)
☞
3.4.2)
☞
3.11.1)
3.6.2)
☞
3.6.3)
3.3.2)