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7
Bright-field Microscopy under the Dia Illumination
Turn on the power switch.
1
When the power to the microscope is turned on, the
microscope starts initialization. And then, the power
indicator on the microscope base is lit. (See Page 34.)
Set the microscope for the bright-field microscopy under the diascopic illumination.
2
If accessories (*1 to *4) for the DIC microscopy under the episcopic illumination are in place, remove
them from the optical path.
1 Push in the optical path selector lever and make
the distribution of light for the binocular part
100%. (See Page 48.)
2 Press the CUBE switch on the operation panel
and light up the "DF (dark-field)" position of the
microscopy method indicator. (See Page 37.)
To use the diascopic illumination, be sure to set
the LV-UEPI2A to "DF."
3 Press the OBJ. switch on the operation panel and
locate the 10x objective into the optical path.
(See Page 43.)
4 Press the DIA switch on the operation panel to
light up the diascopic illumination lamp, and
adjust the brightness with the DIA brightness
switch. (See Page 42.)
5 Locate the NCB11 filter into the optical path and
compensate the color temperature. (See Page 44.)
6 Adjust the brightness with ND filters.
(See Page 44.)
7 Rotate the field diaphragm control toward you
and fully open the field diaphragm for the
diascopic illumination. (See Page 54.)
8 To use the universal condenser, set the condenser
turret to the "O" position. (See Page 55.)
9 Rotate the condenser focus knob to focus the
condenser. (See Page 53.)
10 Rotate the condenser aperture diaphragm ring
toward the left to fully open the condenser
aperture diaphragm. (See Page 54.)
26
1
Turn on
the power.
OBJ .
CUB E
A.S.
2
Set the microscope for the diascopic bright-field
microscopy.
1
F. ST OP
2
BF
DF FL1 FL2
FL1
FL2
*
1
*
2
3
0.8
0.7
Achr N.A
0.6
= 0.9
0.5 0.4
JAPAN
0.3 0.2 0.1
Operation
panel
NCB
ND8
F.S.
7
2
CUBE switch
3
OBJ. switch
EPI
DIA
A.S.
OBJ.
CUBE
4
4
DIA switch
Universal condenser
9
OBJ .
Other condenser
9
OBJ .
0.9
=
0.2
Achr
0.3
0.5
0.4
0.8
0.7
0.6
Power
indicator
EPI
EPI
DIA
DIA
*
3
UEP I2A
*
4
USB
RS2 32C
LCN T
5
6
EPI
DIA
DIA brightness switch
8
10
EPI
EPI
DIA
CUB E
A.S.
DIA
=
0.9
0.2
Achr
0.3
0.6
0.5
0.4
10
0.8
0.7
EPI
EPI
DIA
CUB E
A.S.
DIA
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