Split Ratios - Gilson VERITY 1910 MS User Manual

Split Ratios

The following table provides theoretical values of split ratios for water using the SFI interface.
SFI Split Ratios for Different Waste Tubing Lengths and Diameters
waste Tubing
(length /cm)
25
50
75
100
150
200
250
It is important to note that actual ratios may differ due to various factors, such as liquid
NOTE
composition. Adjustments to tubing lengths may be required.
calculate an appropriate split ratio and attach the required length of pEEK tubing to the
waste port of the splitting tee. Attach the nut, and then finger tighten. The other end can
either be directed to waste or to another analytical instrument, for example, a UV detector.
The split ratios can change if the PEEK tubing from the waste port is attached to another
NOTE
analytical instrument. The theoretical calculations provided in this manual cannot be used for
such systems.
once established, measure the flow rates of different gradient compositions to establish any
variations in flow. Gilson, Inc. recommends that the flow rate throughout the gradient should
be 0.3–2 µl/min.
Split Ratio for
0.005" ID (127 µm)
Tubing
167.8
83.9
55.9
42.0
28.0
21.0
16.8
Split Ratio for
0.007" ID (178 µm)
Tubing
647.6
323.8
215.9
161.9
107.9
81.0
64.8
VERITY® 1910 MS DETEcToR
Split Ratio for
0.01" ID (254 µm)
Tubing
2685.2
1342.6
895.1
671.3
447.5
335.6
268.5
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