Overview - Gilson VERITY 1910 MS User Manual

Overview

In its simplest form, a flow splitter is composed of a union or tee with tubing of various inner
diameters attached as shown in Figure 41.
This appendix describes how to calculate the theoretical flow rates from a ratio of tube
lengths and diameters.
High Flow
Tube
(waste line)
Figure 41
Basic Flow Split
Flow Splitting Guidelines
when creating a split with the split flow interface (SFI), it is important to note the following:
● output flow rates of 0.3–2 µl/min to the spraychip are recommended.
● The split ratio can be adjusted by changing the inner diameter and/or the length of the
waste line (high flow tube).
● The split ratio will vary with the input flow rate and liquid composition (changes during a
gradient).
● Flow rates can be measured using the flow meter assembly. Refer to
Liquid Flow Rate Measurement on page 62.
● For a fixed length, decreasing the diameter of the waste line increases the spraychip flow
rate.
● For a fixed diameter, increasing the length of the waste line increases the spraychip flow
rate.
● changing the diameter of the waste line has a greater effect on the split ratio than
changing the length.
● To reduce the delay time between the liquid system and any associated signal (for
example a UV cell) the length of the fluidic connections between the liquid system and the
SFI should be kept to a minimum.
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Flow SplITTInG
|
VERITY® 1910 MS DETEcToR
Input Flow
(e.g., lc)
low Flow
Tube
(spraychip)