Gilson VERITY 1910 MS User Manual page 11

Split Flow Interface
The split flow interface (SFI) incorporates a built-in splitter that enables you to connect a high
flow system, such as an high pressure liquid chromotography (HPLc) system or MiDas™ to
the VERITY 1910 MS Detector. Gilson recommends using a flow rate range of 10 µL–2 mL/min.
The SFI is also used for auto tuning or calibrating the VERITY 1910 MS Detector. Refer to
Chapter 3 | Auto Tune (Calibrate).
The SFI is shown in Figure 2. It is designed to use 1/32" oD PEEK tubing for the input and
waste lines. Various split ratios can be achieved by connecting a suitable length of PEEK
tubing to the waste (high flow) port. Refer to the FLOW SPLITTING appendix.
connections
Grub
Screw
Figure 2
The Split Flow Interface (SFI)
spraychip® Ion Source
The spraychip is a micro-electrospray ion source. compared to conventional electrospray ion
sources, microspray provides the following advantages: increased ionization efficiency, lower
flow rates, which permits analysis of very small sample volumes over long periods of time,
and lower voltages required to provide efficient electrospray.
Microspray is a soft ionization technique. A voltage, generally between 0.7 and 1.5 kV,
is applied to an analyte dissolved in a suitable solvent, generally a mixture of water and
an organic solvent. The solvent passes through an emitter or capillary tip at a flow rate
of 300–1000 nL/min. The voltages and flow rates used in microspray are lower than for
conventional electrospray ionization. This means that lower volumes of sample and solvent
are consumed by the VERITY 1910 MS Detector.
Under the correct conditions, a plume of charged aerosol droplets forms from a Taylor cone.
Gas-phase analyte ions are subsequently formed from these aerosol droplets.
Ions produced by this method can have single or multiple charges. Generally, high molecular
weight compounds, for example, proteins, produce a series of multiply-charged ions.
Tubing
Main Body
Metal collar
PEEK
Screw
Spraychip
VERITY® 1910 MS DETEcToR
11
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