Sample Handling - Accumetrics VerifyNow User Manual

When using a syringe, immediately transfer blood to the sample tube. Fill to the
3.
black line (Figure 6-2). Do not under fill. If using a needle for sample transfer, the
needle must be 21 gauge or larger (18 gauge preferred).
Figure 6-2 Sample Tube
Fill Volume
Gently invert the tube at least 5 times to ensure complete mixing of the contents.
4.
Samples with evidence of clotting should not be used.
NOTE: Re-draw sample if there is evidence of hemolysis in any other centrifuge
tube that was collected at the same time.
Label the tube with the patient ID, date, and time it was drawn. Dispose of blood
5.
collection supplies according to your institution's policy.
6.3

Sample Handling

Fresh whole blood samples in the appropriate Greiner partial-fill collection tubes are
required for use with the VerifyNow instrument. Keep samples at room temperature.
Do not centrifuge, separate, freeze or refrigerate sample. Table 6-1 provides the
amount of time blood must incubate at room temperature after collection and before
assay. Blood samples older than indicated must be discarded and a new sample
drawn.
Table 6-1 Assay Times
Assay
Aspirin
P2Y12
IIb/IIIa
VerifyNow System User Manual
NOTE: Always ensure collection tubes are filled
to the indicated fill volume.
NOTE: At altitudes greater than 2500 feet above
sea level, blood collection tubes may not fill to
the specified volume, which results in an
incorrect ratio of blood to anticoagulant. Users at
these elevations should use tubes designed for
high altitudes or refer to their facility's blood
collection protocols for instructions to properly fill
blood collection tubes.
Wait Time
30 min
10 min
None
Page 46
PATIENT TESTING - 6
Assay Window
30 min to 4 hrs
10 min to 4 hrs
Within 15 minutes
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