Patient Testing; Patient Preparation; Sample Collection; Peripheral Samples - Accumetrics VerifyNow User Manual

Patient Testing

6
The VerifyNow System has been developed to provide a simple, accurate and
reliable means to measure platelet function from a patient's blood sample. This
section provides instructions on performing the test procedure. The test procedure
includes patient preparation, sample collection, sample handling, preparing the
instrument for use and running a test.
6.1

Patient Preparation

Laboratory testing was performed to determine the effects of several classes of
drugs on VerifyNow Aspirin, P2Y12, and IIb/IIIa results. In addition, laboratory and
clinical testing were performed to assess the effect of the levels of several blood
constituents. Refer to the respective assay package insert for information to be
considered for patients who are to be tested with the VerifyNow assay.
6.2

Sample Collection

Samples can be collected from an indwelling catheter or using direct venipuncture.
NOTE: For an Aspirin or IIb/IIIa platelet function test, whole blood may be collected
from peripheral venous or arterial sites.
6.2.1

Peripheral Samples

Perform the following procedure for sample collection directly into vacuum collection
tubes:
Materials
You will need the following materials:
• Discard tube – may be a tube with no additive, sodium citrate, blood culture,
or heparin tube (IIb/IIIa assay only). The tube cannot contain EDTA (purple
top).
• Aspirin or P2Y12 assay sample tube - 2mL Greiner Bio-One Vacuette
fill blood collection tube with 3.2% sodium citrate - blue top (Greiner #454321)
• IIb/IIIa assay sample tube: use one of the following tubes, depending on the
type of GP IIb/IIIa inhibitor administered.
•
Greiner Bio-One Vacuette partial-fill blood collection tube- lithium heparin
(3 mL fill volume) - green top (Greiner #454082) –for use with Integrilin
(eptifibatide) or ReoPro
•
Greiner Bio-One Vacuette partial-fill blood collection tube (2 mL fill
volume) containing lithium heparin (Greiner #454237) –for use with
Integrilin
VerifyNow System User Manual
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(abciximab)
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(eptifibatide) or ReoPro
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