To Condition A Capillary Column - Agilent Technologies G7035A Operation Manual

To Condition a Capillary Column

Materials needed
• Carrier gas, (99.9995% pure or better)
• Wrench, open-end, 1/4-inch and 5/16-inch (8710-0510)
Do not condition your capillary column with hydrogen. Hydrogen accumulation in
WARNING
the GC oven can result in an explosion. If you plan to use hydrogen as your carrier
gas, first condition the column with ultrapure (99.999% or better) inert gas such as
helium, nitrogen, or argon.
The GC operates under high temperatures. To avoid burns, do not touch any GC parts
WARNING
unless you are certain they are cool.
Procedure
1
2
3
4
5
Never exceed the maximum column temperature, either in the GC/MS interface, the
CA UT IO N
GC oven, or the inlet.
6
7
5977 Series MSD Operation Manual
Install the column in the GC inlet. (See
Split/Splitless Inlet"
Set a minimum velocity of 30 cm/s, or as recommended by the column
manufacturer. Allow gas to flow through the column at room temperature
for 15 to 30 minutes to remove air.
Program the oven from room temperature to the maximum temperature
limit for the column.
Increase the temperature at a rate of 10 to 15 °C/min.
Hold at the maximum temperature for 30 minutes.
Set the GC oven temperature to 30 °C and wait for the GC to become ready.
Attach the column to the GC interface. (See
in the GC/MS Interface"
"To Install a Capillary Column in a
on page 34.)
on page 38.)
Installing GC Columns
"To Install a Capillary Column
2
37