Operation - Qiagen EZ2 Connect User Manual

7.2

Operation

Comments and suggestions
Load check failed Perform camera exposure calibration according to Section 4.3.6.
Both racks must be loaded, even if just one rack is used. Racks have to be the same type.
Check if there is no contamination (e.g. drops) on the labware.
Note: Avoid strong environmental light changes.
Perform load check again.
Hood lock Error Check if the hood is firmly closed. Confirm by pressing down the hood with light force.
Heater Fuse Error Restart the device (several times if necessary).
Pipetting tips sticking to Use lab tissue to wipe the O-rings and make sure weekly maintenance was performed
pipettor correctly.
Pipette tip was crushed
Tip may have been stuck to pipettor. See "pipetting tip sticking to pipettor".
Imprecise pipetting If imprecise pipetting appears over several runs (elution volume): Make sure weekly
maintenance was performed. Check if the tips sits firmly at the pipettor adapter.
Leaking pipette tips Ensure weekly maintenance was performed. Check if the tips sit firmly at the pipettor
adapter.
Pipette tips not picked up Make sure that the tip rack is not damaged and is correctly positioned on the worktable.
by
automatic pipettor
Cross-contamination Ensure maintenance was performed. Clean the piercer and worktable with 70 % ethanol.
Start UV decontamination. Make sure samples and cartridge rack were handled properly.
Update fails
Restart the device and try to start the update again. Make sure you use the QIAGEN USB
flash drive. USB flash drive should stay plugged in during the whole update procedure.
USB device not detected
Restart the device. Save the file(s) to the USB stick again. Check the USB stick on a PC to
ensure it is functional. If the error persists, contact QIAGEN Technical Services.
Connection problem Check if the ethernet cable is connected correctly. Check the Wi-Fi and LAN settings
(Section 5.3.8).
No W-LAN connection Check the Wi-Fi setting (Section 5.3.8). W-LAN adapter should be connected before you
start the device. Reboot the device.
Freeze during run Reboot the device. Restart the protocol run.
Surface damaged Ensure that only the cleaning agents as assumed in Section 6.1 were used.
Display does not turn on Do not touch the display with excessive force or use corrosive chemicals to clean the
display surface. Contact QIAGEN Technical Services for repair.
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