Cleaning, Disinfection, Maintenance; Cleaning; Disinfection At Room Temperature - Leica CM1520 Instructions For Use Manual

Cryostat
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9

Cleaning, Disinfection, Maintenance

9.
Cleaning, Disinfection, Maintenance
9.1

Cleaning

Warning
• Wear protective clothing during cleaning or disinfection work (gloves, face mask, lab coat, etc.).
• Do not use any solvents (xylene, acetone, etc.) for cleaning or disinfection.
• When using detergents and disinfectants, observe the specifications of the respective
manufacturer.
• Risk of explosion when using alcohol: Provide proper ventilation and ensure the instrument is
switched off.
Note
Remove frozen section waste from the cryostat with a cold brush daily.
9.2

Disinfection at room temperature

Warning
• Wear protective clothing during disinfection work (gloves, face mask, lab coat, etc.).
• When using detergents and disinfectants, observe the specifications of the respective
manufacturer.
• Risk of explosion when using alcohol: Provide proper ventilation and ensure the instrument is
switched off.
• Before switching on the instrument again, proper ventilation of the chamber shall be ensured.
Note
• If the cryostat is completely defrosted, the plug on the floor of the cryochamber must be removed.
Afterward, reinsert rubber plug and check that it is securely in place.
• The defrosting fluid is drained to a separate waste container alongside the instrument through a
drain tube (→ "Fig. 54-1").
1. Switch off the instrument.
2. Carefully remove the knife/disposable blade as well as specimens and accessories from the
chamber.
3. Remove the section waste from the cryochamber and dispose of it.
4. Remove the closed sliding window from the front by gently lifting it
(→ P. 70 – 9.3.3 Removing the sliding window).
5. Clean the chamber walls using a paper towel moistened with an alcohol-based disinfectant.
6. Place a suitable waste container underneath the drain tube at the right side of the instrument
(→ "Fig. 54-1").
7. Pull the rubber plug out of the bottom of the chamber and drain the disinfectant into the waste
container.
8. Use the rubber plug to re-seal the drain in the cryochamber.
9. Dispose of liquid according to the waste disposal regulations.
10. Allow the cryochamber to dry thoroughly.
66
Version 2.0, Revision K

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