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Problem
Uneven sections.
Chatter during sectioning.
Condensation on anti-roll plate
and knife during cleaning.
Anti-roll plate damaged after
adjustment.
Leica CM1520
Cause
• Knife damaged.
• Edge of anti-roll plate
damaged.
• Specimen insufficiently frozen
onto the specimen disc.
• Specimen disc not clamped
tightly.
• Ball-and-socket joint of the
holder not clamped into place
tightly.
• Knife not clamped tightly
enough.
• Specimen has been sectioned
too thickly and has detached
from the disc.
• Very hard, inhomogeneous
specimen.
• Dull knife.
• Knife profile inappropriate for
this specimen used.
• Incorrect clearance angle.
• Brush, forceps, cloth or other
cleaning materials too warm.
• Anti-roll plate too high above
the knife edge. The adjustment
was carried out in the direction
of the knife.
Troubleshooting
Remedy
• Use a different part of the
knife.
• Replace anti-roll plate.
• Refreeze specimen onto the
disc.
• Check clamping.
• Check clamping of the ball-
and-socket joint.
• Check knife clamping.
• Refreeze specimen onto the
disc.
• Increase section thickness;
reduce specimen surface area
if necessary.
• Use a different part of the
knife.
• Use knife with different profile.
• Make clearance angle
adjustment.
• Use only cooled components
and clean ing materials. Store
all tools on storage shelf in the
cryochamber.
• Replace anti-roll plate; then lift
it during adjustment.
• Handle anti-roll plate more
carefully!
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