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8
Troubleshooting
Problem
Thick/thin sections.
Tissue adheres to the anti-roll
plate.
Flattened sections curl up when
anti-roll plate is folded up.
Sections tear or separate.
Inconsistent or inaccurate
specimen feed.
64
Cause
• Temperature incorrect for the
tissue to be sectioned.
• Knife profile being used
is inappropriate for this
specimen.
• Ice formation on back of knife. • Remove ice.
• Handwheel speed not uniform
or turned at incorrect speed.
• Knife not clamped tightly
enough.
• Specimen holder not clamped
tightly.
• Cryocompound applied to cold
specimen disc; specimen can
detach from the disc after
freezing.
• Dull knife.
• Incorrect clearance angle.
• Dried specimen.
• Anti-roll plate is too warm or
incorrectly positioned.
• Static charge.
• Grease on the corner or edge
of anti-roll plate.
• Rust on the knife.
• Static charge or air current.
• Anti-roll plate too warm.
• Temperature too low for the
tissue being sectioned.
• Dull part, dirt, dust, frost or
rust on the knife.
• Top edge of the anti-roll plate
damaged.
• Hard particles in the tissue.
• Dirt on back of knife.
• Nick in the cutting edge of the
blade or knife.
• Microtome defective.
Remedy
• Select the correct temperature
and wait until the correct
temperature is reached.
• Use knife with different profile
(c or d) or switch to disposable
blades if necessary.
• Adapt speed.
• Check clamping.
• Check clamping.
• Apply cryocompound on
lukewarm disc; mount
specimen in cryochamber and
freeze it.
• Use a different part of the
knife.
• Set the correct clearance
angle.
• Prepare new specimen.
• Cool down anti-roll plate or
reposition correctly.
• Remove static charge.
• Remove grease using alcohol.
• Remove rust.
• Remove static charge.
• Cool down anti-roll plate.
• Select higher temperature and
wait.
• Remove cause, clean.
• Replace anti-roll plate.
• If the application permits, cut
deeper during sectioning.
• Clean.
• Use adifferent part of the
blade/knife.
• Call technical service.
Version 2.0, Revision K
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