Create Dark And Bias Images - Thermo Scientific myECL Imager Manual

Create Dark and Bias Images

22 ECL Imager User Manual
MY
Dark current is the thermal noise generated from a CCD (charge coupled device).
This thermal noise is proportional to a power law of CCD temperature. The bias
noise is introduced as the analog signal is converted to a digital signal. The
Imager applies dark and bias noise compensation using dark and bias masters upon
image acquisition.
This function allows the administrator to create new dark and bias master files. Dark
and bias masters need to be updated only when strong CCD noise appears on
acquired images. It takes ~14 hours to create all of the dark and bias masters in the
system; therefore, creation of new dark and bias masters is best accomplished by
beginning the process at the end of the day and allowing the masters to be created
overnight.
1. Select the Tools button.
2. Select the Administrator Settings button. Enter the User Name and Password.
Select the Login button.
3. Select the Create Dark and Bias Images button. The following screen is
displayed:
4. Select Start. To create only dark or only bias masters, choose the corresponding
check box next to the desired selection.
Note Select the Cancel button to return to the Administrator Settings screen.
5. Place the black sample screen on the transilluminator and close the door.
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