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Related Manuals for Thermo Scientific MR 23i
Summary of Contents for Thermo Scientific MR 23i
- Page 1 Instruction Manual MR 23i Analyze · Detect · Measure · Control™ P/N 50116911-1...
- Page 3 Instruction Manual Jouan MR 23i PN 50116911-1 Issued 06/08...
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Page 5: Table Of Contents
Table of contents Chapter 1 USE AND FUNCTION ............1-1 DESCRIPTION ..............1-1 RELATIVE CENTRIFUGAL FORCE........1-1 Centrifugation formulae............1-1 Chapter 2 INSTALLATION PROCEDURE ........2-1 LIFTING AND TRANSPORT ..........2-1 UNPACKING..............2-1 MAINS SUPPLY ..............2-2 ENVIRONMENTAL CONDITIONS ........2-2 POSITIONING ..............2-2 LID OPENING..............2-2 MANUAL LID UNLOCKING ..........2-2 INSPECTION ..............2-3 PREPARATION ..............2-3 SAMPLE LOADING ............2-4... - Page 6 OPERATIONAL LIMITATIONS ..........6-15 ELECTRICAL..............6-16 IMPROPER USE ...............6-16 BIOLOGICAL RISKS ............6-16 Chapter 7 SERVICING AND PREVENTATIVE MAINTENANCE ..7-1 CLEANING.................7-1 PERIODIC PREVENTATIVE MAINTENANCE ....7-1 CIRCUIT BREAKER ............7-1 Annex A GUARANTEE TERMS .............A-1 MR23i Thermo Fisher Scientific...
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Page 7: Chapter 1 Use And Function
Chapter 1 USE AND FUNCTION USE AND FUNCTION Chapter 1 DESCRIPTION The Jouan MR23i is a high speed centrifuge (23 000 rpm) desi- gned to separate substances by elevated centrifugal acceleration. It is equally suited to precise, low speed operation. Different rotors can be fitted to the drive in order to match the sam- ple containers and the required performance. - Page 8 Chapter 1 USE AND FUNCTION Primary calculations Key sequence (not valid for CASIO, HP) RCF xg 1.118RN x1.118xR= ÷ Speed (rpm) ----------- - RCF R x946 ÷ Radius (mm) ---------------------- x1.118 1.118N Transformations Key sequence To determine actual ‘g’ achieved at a different speed: ⎛...
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Page 9: Chapter 2 Installation Procedure
Chapter 2 INSTALLATION PROCEDURE INSTALLATION Chapter 2 PROCEDURE LIFTING AND Due to the weight of the machine, all lifting and transporting must TRANSPORT be carried out using proper handling equipment (eg: fork lift trolley) that complies with current regulations, and by people having under- gone the necessary training. -
Page 10: Mains Supply
Chapter 2 INSTALLATION PROCEDURE MAINS SUPPLY The Jouan MR23i requires a single phase supply: 11174729 220/240 V 50 Hz + earth 11174730 120 V 60 Hz 11 A + earth 11174731 230 V 60 Hz + earth Maximum power requirement : 1300 VA (average: 1000 VA). The centrifuge should be connected to a 3 wire earthed outlet. - Page 11 Chapter 2 INSTALLATION PROCEDURE During rotation, the slightest leak from a sample is enough to create an aerosol. Rotation of the rotor can only be detected when the machine is switched on. Opening the lid manually if the rotor is still rotating, would disperse the aerosol in the environment.
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Page 12: Inspection
Chapter 2 INSTALLATION PROCEDURE INSPECTION Before installation, the rotor should be thoroughly inspected for cor- rosion and cleanliness. Chemical and stress corrosion will eventually lead to disruption of the rotor with potential severe damage to the centrifuge. Particles stuck inside the pockets can cause breakage of tubes and lead to major imbalance and / or loss of sample and contamination. -
Page 13: Rotor Removal
Chapter 2 INSTALLATION PROCEDURE It is not necessary to orientate the rotor relative to the drive shaft in order to achieve locking. The AUTOLOCK rotor mounting system allows rotors to be placed in any orientation. YOU ARE STRONGLY ADVISED TO USE SEALED LIDS ON ROTORS IN ORDER TO BE PROTECTED AGAINST THE BIOLO- GICAL HAZARDS RESULTING FROM BROKEN OR LEAKING TUBES AND BOTTLES. - Page 14 Chapter 2 INSTALLATION PROCEDURE MR23i Thermo Fisher Scientific...
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Page 15: Chapter 3 Specifications
Chapter 3 SPECIFICATIONS SPECIFICATIONS Chapter 3 DIMENSIONS AND WEIGHT Dimensions (H x W x D) 360 x 585 x 635 mm (16.5 x 23 x 25 in.) Packed (H x W x D) 510 x 720 x 790 mm (22.4 x 28.4 x 31.1 in.) Weight - uncrated / crated 85 kg (188 lb) / 100 kg (221 lb) -
Page 16: Acceleration Rate Control
Chapter 3 SPECIFICATIONS Integrator Range 100 to 281 x 10 Step 1 m. rd Acceleration rates Braking rates Temperature Range -8°C to +40°C Step 1°C Accuracy ± 1.5°C Typical -5°C at 4400 rpm, 3074 x g (4 x 200 ml performance swing-out) 1°C at 23000 rpm, 34780 x g (12 x 1.5 ml angle) -
Page 17: Rotor Selection Table
Chapter 3 SPECIFICATIONS DRM 6.14 45 sec. AM 38.15 70 sec. AM 2.23 30 sec. AM 50C.13 155 sec. AM 2.18 35 sec. AM 50.14 140 sec. AM 2.19 40 sec. AM 100.13 170 sec FAJM 2.15C 45 sec. SWM 180.5 60 sec. - Page 18 Chapter 3 SPECIFICATIONS 11174722 Trans. sealing lid 11174611 Set of 8 adaptors 1 x 16 ml 18 x 100 15300 22507 11174601 Set of 8 adaptors 1 x 13 ml 17 x 85 15300 21984 11174602 Set of 8 adaptors 1 x 10 ml 16 x 80 15300...
- Page 19 Chapter 3 SPECIFICATIONS 11174533 Set of 4 inserts 5 x Z5, 5 ml 17 x 60 4400 3074 11174504 Set of 4 inserts 5 x 5-7 ml Ø 13.5 4400 3074 11174505 Set of 4 inserts 9 x 5-6 ml Ø...
- Page 20 Chapter 3 SPECIFICATIONS • If the chamber was more than 10°C, but temperature setting at 4°C and speed at 13 000 rpm, chamber temperature will never decrease. When running at ultimate conditions (13 000 rpm and temperature below 8°C) cycling speed variations can be observed of about ±200 rpm which does not affect the efficiency of centrifugation.
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Page 21: Chapter 4 Operating Principales
Chapter 4 OPERATING PRINCIPALES OPERATING Chapter 4 PRINCIPALES DRIVE SYSTEM The rotor is driven by a three phase asynchronous motor. The rotor is situated in the centre of a virtually hermetically sealed and armour plated centifugation chamber. A control system ensures the correct drive speed. -
Page 22: Refrigeration System
Chapter 4 OPERATING PRINCIPALES The imbalance condition is displayed with the message “IMBA- LANCE”. The display shows the actual speed and a TIME OUT delay. The operator cannot open the lid until the rotor has stopped spinning and he has pressed the ENTER key. When the motor stops, opening and correctly loading the rotor allows the unit to be restarted. - Page 23 Chapter 4 OPERATING PRINCIPALES terms of motor rotation speed (rpm). A given rotation speed corre- sponds to centrifugal acceleration which is directly proportional to the centrifugation radius, in keeping with the following equation: Number of „g“ 1.118 Acceleration Centrifugation Speed in thou- radius (mm) sands of rpm For example, a given separation may be described as obtainable...
- Page 24 Chapter 4 OPERATING PRINCIPALES The integral of the curve is the value of its area. The area repre- INTEGRAL OF THE CURVE sents the total amount of separating force applied to the sample. Under the previous conditions, calculation of this integral is very simple.
- Page 25 Chapter 4 OPERATING PRINCIPALES Integration control allows suppression of the effect of the various potential fluctuations. Indeed, rather than programming a given duration of centrifugation, it is possible to set the desired value of the integral for a given speed or centrifugal acceleration level (R.C.F.).
- Page 26 Chapter 4 OPERATING PRINCIPALES MR23i Thermo Fisher Scientific...
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Page 27: Instructions For Use
Chapter 5 INSTRUCTIONS FOR USE INSTRUCTIONS FOR Chapter 5 CONTROLS AND INDICATORS The mains switch is located on the lower left side of the front panel. The control system includes a dot-matrix graphic LCD screen, able to display numbers, letters, and special characters in addition to animated graphics. - Page 28 Chapter 5 INSTRUCTIONS FOR USE Moves the indicator from one parameter to another to allow values to be altered. ABOUT Shows a graphical representation of the integral, INTEGRAL together with values calculated from the previous run (where possible). Enables the acceleration rate to be altered bet- ween the 10 choices from 0 (slowest) to 9 (fastest).
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Page 29: Use
Chapter 5 INSTRUCTIONS FOR USE NONE Selects “NO PASSWORD PROTECTION” when memorizing an application. PRE COOL Starts a predetermined precooling cycle (2000 rpm, 4°C, 15 min). During this cycle all other pro- gramming facilities are operational. RADIUS Enables the effective radius of a selected rotor to be modified in order to allow for the use of an adaptor or to select a boundary at which a set “g”... - Page 30 Chapter 5 INSTRUCTIONS FOR USE A keypress on CE will cause the display of the value of the actual PRECOOLING temperature of the air in the bowl to the left of the set value during the time that the CE key is pressed. If the operator wishes to precool the bowl and rotor, he simply sel- ects PRE COOL having installed the rotor, and the Jouan MR23i runs according to the pre-set values which are indicated on the...
- Page 31 Chapter 5 INSTRUCTIONS FOR USE During a run, the basic screen displays the 3 basic parameters with both set and live values. A keypress on EXPAND DISPLAY presents a detailed screen showing all possible run parameters and the values of those selec- ted, both set and live.
- Page 32 Chapter 5 INSTRUCTIONS FOR USE Note If they had been programmed previously, RCF could replace speed and integral could replace time in the basic parameter menu. Note In either case, the new values are held in a buffer memory (identified END PROG or NEW by - -) where they remain until the next time APPLI is used.
- Page 33 Chapter 5 INSTRUCTIONS FOR USE The flashing arrowhead indicates T (For an explanation of Chapter 4: “OPERATING PRINCIPALES” Section “REFRIGE- RATION SYSTEM”). Use the keypad to input a value and then (if required) the +/- key to change the mathematical sign of the value. Alternatively, the user has the choice of directly selecting one of the most popular operating temperatures (4, 20 or 37°C) and/or using +/- to introduce a negative value.
- Page 34 Chapter 5 INSTRUCTIONS FOR USE Select ENTER to save the application in the indicated file. The user is offered the opportunity to enter an individual password for this application (accepts 0 to 9999 inclusive, via the keypad, then select ENTER) or to select NONE for no password protection to save the application.
- Page 35 Chapter 5 INSTRUCTIONS FOR USE B) During a run From the basic or detailed run display, select MODIFY. The three main control parameters (speed or RCF/ temperature/time or inte- gral) will be displayed together with the current set values. to move the cursor to the parameter to be modified and enter the value via the keypad.
- Page 36 Chapter 5 INSTRUCTIONS FOR USE NOTE: Never mix buckets from different sets on the same rotor. If the message persists, call an engineer. LID UNLOCKED: Displayed if the lid was not correctly shut. The instrument will inform you to close it correctly in order that it can start the requested run.
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Page 37: Hazards, Precautions And Limitations Of Use
Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE HAZARDS, Chapter 6 PRECAUTIONS AND LIMITATIONS OF USE CAUTIONS • Never try to bypass the lid lock safety while the rotor is spin- ning. • Do not try to open the lid until display returns to the stand-by mode shown when machine is switched on. - Page 38 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE Chemical Corrosion This corrosion is characterised by chemical reactions due to the existence of an electrolyte liquid on the surface of the item. If these substances are allowed to remain on the surface the corrosion will almost certainly occur.
- Page 39 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE Stress Corrosion This term relates to the phenomenom of accelerated corrosion due to the effect of centrifugation when a corrosive chemical is in con- tact with the alloy. From the time when the aluminium alloy has been attacked by chemicals, stress corrosion begins to appear.
- Page 40 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE Once the rotor is clean, rinse it in running water, preferably distilled. Dry the rotor with a soft absorbant non-woven cloth or tissue. Drying may be finished off with warm air jet (e.g. a hair dryer). MAKE CERTAIN THAT THE POCKETS ARE WELL DRIED.
- Page 41 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE ANY PART WHICH HAS BEEN SUBJECTED TO TEMPERA- TURES ABOVE 130°C MUST BE DISCARDED. Hypochlorite “bleach” used at 0.1% concentration with 10 minutes immersion is effective against bacteria, spores and viruses but, as an oxidising agent, is corrosive to metal alloys and must be thorou- ghly rinsed off and dried.
- Page 42 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE Thermo Fisher Scientific is able to supply cans of Anti Corrosion Spray, catalogue N° 11175399, whose propellant drives out water-based moisture and leaves a protective lanolin film on the surface. It is strongly recommended that rotors not in use should be stored STORAGE OF ROTORS upside down on a non-metallic grid or other support which allows free movement of air.
- Page 43 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE Acetic Acid (80 %) Acetic Acid (Glacial) Acetic Anhydride Acetone Acetonitrile Acetylene Adipic Acid Alanine Allyl Alcohol Aluminium Chloride Aluminium Fluoride Aluminium Hydroxide Aluminium Nitrate Aluminium Sulphate Amino Acids Ammonia Ammonium Acetate Ammonium Carbonate Ammonium Chloride...
- Page 44 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE Barium Hydroxide Barium Sulphate Benzaldehyde Benzene Benzoic Acid, Sat Benzyl Acetate Benzyl Alcohol Boric Acid Bromine Bromobenzene Bromoform Butadiene Butane n-Butanol n-Butyl Acetate Butylene Butyl Chloride Butyric Acid Caesium Acetate Caesium Bromide Caesium Chloride Caesium Formiate Caesium Iodide...
- Page 45 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE Chlorine, moist Chloroacetic Acid p-Chloroacetopheno Chlorobenzene Chloroform Chlorosulphonic Chromic Acid (5 %) Chromic Acid (10%) Chromic Acid (50%) Cinnamon Essence Citric Acid (10%) Copper Nitrate Copper Sulphate Croesol Cyclohexane Cyclohexanol Cyclohexanone Cyclopentane Decane Dextran Sulphate Diacetone...
- Page 46 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE Dimethylformamide Dimethylsulphoxide Dioxane Diphenyloxide Dipropylene Glycol Distilled Water Ethanol (50%) Ethanol (95%) Ethyl Acetate Ethyl Benzene Ethyl Benzoate Ethyl Butyrate Ethyl Chloride Ethylene Chloride Ethylene Glycol Ethylene Oxide Ethyl Ether Ethyl Lactate Ethyl Malonate Fatty Acids Ferric Chloride...
- Page 47 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE Glutaraldehyde Glycerine Glycerol Heptane Hexane Hydrazine Hydrochloric Acid (5%) Hydrochloric Acid (37 %) Hydrochloric Acid (50%) Hydrochloric Acid (conc). Hydrofluoric Acid (10%) Hydrofluoric Acid (50%) Hydrogen Peroxide (3%) Hydrogen Peroxide (100%) Iodine, Crystals Isobutyl Alcohol Isopropyl Alcohol Isopropylbenzene...
- Page 48 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE Mercury Methanol (100%) Methyl Acetate Methyl Alcohol (100%) Methyl Butyl Ketone Methyl Ethyl Ketone Methyl Isobutyl Ketone Methyl Isopropyl Ketone Methylene Chloride Mineral Oil Nickel Chloride Nickel Salts Nickel Sulphate Nitric Acid (10%) Nitric Acid (20%) Nitric Acid (50%) Nitric Acid (95%)
- Page 49 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE Perchloric Acid Perchloric Acid (10%) Perchloric Acid (70%) Petrol Phenol (5%) Phenol (50%) Phenol, crystals Phenol, liquid Phenyl Ethyl Alcohol Phosphoric Acid (10%) Phosphoric Acid (conc.) Picric Acid Pine Oil Potash, conc. Potassium Bromide Potassium Carbonate Potassium Chlorate...
- Page 50 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE Salycylic Acid, Sat Serum Silver Acetate Silver Nitrate Sodium Acetate Sodium Bisulphate Sodium Borate Sodium Bromide Sodium Carbonate (2%) Sodium Chloride (10%) Sodium Chloride (Sat’d.) Sodium Hydroxide (>1%) Sodium Hydroxide (10%) Sodium Hydroxide (conc.) Sodium Hypochlorite (5%)
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Page 51: Iec 1010-2-020 Standard
Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE Tetrahydrofuran Thionyl Chloride Toluene Trichlorethylene Trichloroacetic Acid 1,2,4 - Trichlorobenzen Trichloroethane Triethylamine Triethylene Glycol Tris Buffer (neutral) Trisodium Phosphate Triton X-10 Turpentine Undecyl Alcohol Urea Urine Vinylidene chloride Xylene Zinc Chloride Zinc Hydrosulphite Zinc Sulphate Polypropylene Copolymer This table is intended as a guide only because of the difficulty in... -
Page 52: Operational Limitations
Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE • To mark out a clearance envelope 30 cm around the centrifuge or establish special procedures for the non entry of all persons or all dangerous materials into this space during the operation of the centrifuge. - Page 53 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE • A ventilated centrifuge (without built-in refrigeration) perma- nently exhausts into the room air which has passed through its bowl while a refrigerated (or thermostated) centrifuge contains a relatively sealed bowl. Thus the risks from the spread of aero- sols are minimised in a refrigerated centrifuge and significant in a ventilated centrifuge.
- Page 54 Chapter 6 HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE 6-18 MR23i Thermo Fisher Scientific...
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Page 55: Chapter 7 Servicing And Preventative Maintenance
Chapter 7 SERVICING AND PREVENTATIVE MAINTENANCE SERVICING AND Chapter 7 PREVENTATIVE MAINTENANCE CAUTION: All cleaning should be performed with the centrifuge disconnected from the power outlet. CLEANING Daily No daily cleaning is required, except for accidental glass breakage, or a large amount of spillage in the rotor chamber. Weekly Clean the rotor chamber and the accessories with a cotton wool pad dipped in 70% alcohol. -
Page 56: Circuit Breaker
Chapter 7 SERVICING AND PREVENTATIVE MAINTENANCE CIRCUIT If the main circuit breaker cuts off the power to the instrument, do BREAKER not attempt to switch on before a service engineer has checked over the unit. MR23i Thermo Fisher Scientific... - Page 57 Annex A GUARANTEE TERMS GUARANTEE TERMS Annex A Thermo Fisher Scientific guarantees that this unit is free from defects in materials and workmanship when it leaves the factory, and undertakes to replace or repair the unit if it proves defective in normal use or during servicing for a period of ONE YEAR from the delivery.
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Page 58: Annex Aguarantee Terms
Annex A GUARANTEE TERMS MR23i Thermo Fisher Scientific... - Page 59 Annex B Declaration of conformity Declaration of confor- Annex B mity Great Britain WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96 It is marked by the symbol opposite. Thermo Fisher Scien- tific has entered into agreements with recycling and disposal compa- nies in all EU Member States for the recycling and disposal of this device.
- Page 60 Annex B Declaration of conformity España Conformidad con WEEE. Este producto cumple con las disposicio- nes de la Directiva europea sobre equipos eléctricos y electrónicos usados (Directiva WEEE 2002/96). Ello se indica con el símbolo al margen. Thermo Fisher Scientific tiene firmados acuerdos con varias empresas de reciclaje y eliminación de residuos de todos los esta- dos miembros de la U.E.
- Page 61 Annex B Declaration of conformity Portugal Conformidade com a Diretiva Européia REEE. Este produto está sujeito às determinações da Diretiva Européia de Resíduos de Equi- pamentos Elétricos e Eletrônicos (Diretiva REEE 2002/96). Isto é caracterizado pelo símbolo ao lado. Thermo Fisher Scientific A fechou um acordo com todas as empresas de reciclagem e de elimi- nação de resíduos em todos os Estados-Membros da UE para que o produto seja reciclado ou eliminado.
- Page 62 Annex B Declaration of conformity Biofuge Primo R Heraeus Thermo Fisher Scientific...
- Page 63 Index Index Password Protection ........5-7 Performance ............3-1 Periodic Preventative Maintenance ....7-1 Positioning ............2-2 Acceleration rate control ......... 3-2 Precooling ............5-4 Preparation ............2-4 Preventative Maintenance .......6-5 Biological Risks ..........6-16 Programming of Parameters ......5-5 Braking rate control ........3-2 Relative Centrifugal Force .......1-1 Cautions ............
- Page 64 Index MR23i Thermo Fisher Scientific...
- Page 66 CHINA INDIA JAPAN Tel.: +86 21 68 65 45 88 Tel.: +91 22 55 42 94 94 Tel.: +81 454 53 92 20 Tel.: +86 10 58 50 35 88 OTHER ASIA PACIFIC COUNTRIES Tel.: +852 28 85 46 13 AUSTRIA BELGIUM FINLAND...