17
ECL Imager User Manual
MY
3. When complete, select the Save button at the bottom of the menu to save the
changed defaults.
Table 2-5. Imager filter specifications and corresponding dyes.
Filter Color
Wavelength Range
(nm)
Blue
445-480
Green
515-560
Orange
545-630
Red
620-645
§
With the
ECL Imager, the orange filter is optimal for Hoechst Blue imaging.
MY
†
Coomassie Blue and Thermo Scientific Imperial Stains do not require the orange filter;
however, these stains require the white light conversion screen. UV and Vis modes use the
orange filter in Position 2 by default.
Compatible Dyes
and Stains
DAPI
Clear screen
AMCA
Clear screen
SYBR* Green
Clear screen
SYBR Gold
Clear screen
Ethidium Bromide
Clear screen
§
Hoechst Blue
Clear screen
Coomassie Blue
White screen
Imperial*
White screen
SYPRO* Ruby
Clear screen
Deep Purple*
Clear screen
Sample Screen
†
†
Thermo Fisher Scientific