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Summary of Contents for Thermo Scientific Matrix MultiChannel Equalizer Pipette
- Page 1 Thermo Scientific Matrix MultiChannel Equalizer Pipette with Expandable, Equal Tip Spacing User Manual...
- Page 2 Thermo Scientific Matrix Equalizer MultiChannel Electronic Pipette with Expandable, Equal Tip Spacing © 2011 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. All other trademarks and product names are the intellectual/ industrial property of their respective owners.
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Page 3: Table Of Contents
Content Congratulations! ......................1 Before You Begin ......................1 A. INTRODUCTION ..................2 A.1 Thermo Scientific Matrix Equalizer™ Pipette Models ........3 B. PIPETTE OVERVIEW ................. 3 B.1 Components ......................3 B.2 General Description ....................4 B.3 Keypad ........................5 B.3.1 Operation Keys ....................5 B.3.2 Programming Keys ...................6 B.3.3 Special Function Key ..................7... - Page 4 D. CALIBRATION ..................23 D.1 Volume Measurement ..................23 D.2 Pipette Calibration .....................24 D.2.1 Factory Calibration Step ................24 D.2.2 “EASY” Cal Mode ...................25 D.2.3 “CALC” Mode ....................25 D.2.4 CALVIEW .......................26 D.2.5 CALRESET ....................27 D.3 Recommendations for Accurate Pipetting ............27 E. TROUBLESHOOTING ................29 F.
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Page 6: Congratulations
Congratulations! You have purchased the state-of-the-art in precision pipetting. The patented* Thermo Scientific Matrix Equalizer Pipette is available with variable, equal tip spacing, allowing multiple sample delivery between various tube rack, vial and plate formats. Ergonomically designed and balanced to reduce fatigue, the Matrix Equalizer pipette functions so intuitively you may need only a quick survey of this manual to use it. -
Page 7: Introduction
Introduction A. INTRODUCTION The revolutionary Thermo Scientific Matrix Equalizer Pipette features a patented* tip spacing mechanism to boost laboratory productivity while reducing risk of repetitive stress injuries (RSI). Built with the same design principles incorporated into Thermo Scientific Matrix Electronic Pipettes, the Matrix Equalizer pipette features include: •... -
Page 8: Thermo Scientific Matrix Equalizer™ Pipette Models
Pipette Overview A.1 Equalizer Pipette Models Matrix Equalizer multichannel pipettes are available in a number of configurations in both 8 and 12 channnels. Models range in volume from 0.5 µl to 1250 µl. Each handle is color-coded with a color ring for easy volume identification (see section B.5, Handle Specifications). -
Page 9: General Description
Pipette Overview B.2 General Description Keypad. The keypad contains the operation keys that are used in programming the pipetting sequence and volumes. (See Section B.3, Keypad). Display. The display is an LCD screen that shows the pipetting operation (fill, dispense, mix, or purge) and fluid volume for each step in the pipetting program. -
Page 10: Keypad
Pipette Overview B.3 Keypad The keypad contains the operation keys, programming keys, and the special function key. Figure B.3: Equalizer Keypad B.3.1 Operation Keys [On/Off] The On/Off key turns the pipette on or off. When the pipette is turned on, it emits a beep tone, flashes “ZERO” on the display, then automatically resets the pistons to zero position. -
Page 11: Programming Keys
Pipette Overview [Purge] The Purge key enters a purge prompt anytime during a pipetting operation to abort the program. By pressing the Purge key, then pressing the trigger, you can dispense any remaining liquid from the pipette tips and return to the beginning of the program. The Purge key is also used to enter the purge step into a program. -
Page 12: Special Function Key
Pipette Overview The Volume key controls the quantity of liquid to be aspirated or dispensed. The volume can be entered when the “Vol” prompt appears. Pipetting volume can be incremented quickly by continuously depressing the Volume key. The longer you hold the key the greater the incremental speed. - Page 13 Pipette Overview Double beep tone: operation or programming key is not active in current mode; insufficient dispense volume, purge required; illegal programming step attempted (see Section E, Troubleshooting). Triple beep tone: end of pipetting sequence; end of programming sequence. There are three options to choose from in setting the beep tone.
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Page 14: Display
Pipette Overview B.4 Display The pipette display is a dynamic LCD screen. It displays the current operation, volume prompt (if entering a program), pipetting volume, beep tone symbol (if the beep tone is on), pipetting speed, and program number. Figure B.4: Equalizer Display... -
Page 15: Matrix Equalizer Pipette Specifications
Pipette Overview B.5 Equalizer Pipette Specifications Item Volume Color Accuracy* Precision** Volume Channel Range Ring (±) (±) 2032 250 µl 8-Ch 5-250 µl Blue 2.5%/1.5 µl 0.7%/1.0 µl 2034 1250 µl 8-Ch 15-1250 µl Green 1.5%/6.0 µl 0.6%/3.0 µl 2139 12.50 µl 8-Ch 384 0.50-12.50 µl... -
Page 16: Hazards And Precautions
Pipette Overview B.6 Hazards and Precautions There are no known hazards associated with the Equalizer pipette when it is operated in accordance with the instructions in this manual. However, you should be aware of situations that can result in serious injury. Note: Do not perform troubleshooting procedures on the internal components unless instructed by Thermo Fisher Scientific Technical Service personnel. -
Page 17: Programming And Operation
Programming and Operation C. PROGRAMMING AND OPERATION C.1 Operating the Equalizer Pipette Figue C.1: Equalizer Spacing The Matrix Equalizer pipette allows the tip-to-tip spacing to change. This allows pipetting into or between vessels of varying center-to-center configurations. The set button(s), spacing indicator and spacing control rod all work together to change the distance between the tips (see figure C.1). - Page 18 Programming and Operation Matrix Equalizer 384 Pipette: 1. To set the tip spacing for the vessel with the widest spacing, depress the outer set button (the button closest to the control rod) using your finger or thumb. 2. While depressing, slide the set button to the desired tip-to-tip spacing marker on the window.
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Page 19: Programming The Equalizer Pipette
Programming and Operation C.2 Programming the Equalizer Pipette Programming the Equalizer pipette is fast and easy. Pipetting steps and volume are entered in the sequence that they will be performed, then the program is saved to memory. This program will stay in memory (even if the pipette is turned off, or the battery runs low) until you change it. - Page 20 Programming and Operation 2. Select pipetting operation. Enter the programming mode by pressing the [Fill] or [Mix] key. For example, to begin programming with a filling operation, press the [Fill] key. The display shows “Fill” with a Figure C.2.2a: Flashing Volume Display flashing “Vol”...
- Page 21 Programming and Operation If the program begins a mix cycle above zero position (volume is greater than zero), the fluid is dispensed, and the mix cycle begins. The mix cycle will end at the same volume it started with. Also, the mix operation will remain in the display so it can be repeated (useful for serial dilutions), unless followed by another program step.
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Page 22: Storing A Program
Programming and Operation 6. Save program and exit. When the display flashes all operation keys, press [Enter] to save the program and exit the programming mode. The pipette emits a triple beep tone to signal the end of the programming sequence. -
Page 23: Adjusting Pipetting Speed
Programming and Operation C.2.5 Adjusting Pipetting Speed The Matrix Equalizer pipette offers unique speed control programming for Fill, Dispense, Mix, or Purge. Speeds within any of the five stored programs can be independently set and stored as an integral part of the pipetting protocol. Pipetting speed is easily changed at the start of each pipetting function. -
Page 24: Programming Examples
Programming and Operation C.3 Programming Examples Examples of different pipetting sequences are described below, and are ideal for use as training exercises. Press the keys shown in keycaps [ ], then press the [Vol] key until the volume in parentheses ( ) is displayed. At the end of the program, a triple beep tone is sounded. -
Page 25: Serial Dilutions
Programming and Operation In this program, only one dispense step needs to be programmed. If any program ends in a dispense step, the pipette repeats the dispense step until the volume is zero, or is less than the programmed dispense volume. If the remaining volume is less than the programmed dispense volume, the pipette displays a “Purge”... -
Page 26: Supernatant Collection
Programming and Operation C.3.5 Supernatant Collection Example: Aspirate 100 µl from 8 rows of a Microtiter plate and discard the ® total volume. This program is performed with the 1250 µl Equalizer pipette. All 100 µl fill volumes are [Fill] (100) [Enter] collected in the same tips. - Page 27 Programming and Operation 1. Attach pipette tips. When ready to begin pipetting, attach the tips to the pipette. Note: When applying tips, avoid ‘banging’ or applying excessive force to the tip(s), as this stretches the tip seal and will affect volume. Use Matrix tips which have been specifically designed to match the tip fittings on Matrix pipettes.
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Page 28: Calibration
Calibration D. CALIBRATION The Equalizer pipette is factory-calibrated for distilled water at 20 °C. For easy calibration using distilled water, the Equalizer pipette features an “EASY” CAL mode (see Section D.2.2, “EASY” CAL mode). When pipetting liquids of significantly different specific gravities or temperatures, however, the Calibration (CALC) feature may be used to achieve greater accuracy (see Section D.2.3, “CALC”... -
Page 29: Pipette Calibration
Calibration D.2 Pipette Calibration Equalizer pipette users can calibrate their pipettes with “EASY” CAL mode for distilled water or with “CALC” mode to calibrate other liquids and for temperatures other than room temperature. D.2.1 Factory Calibration Step The factory calibration step is needed when calibrating your Equalizer pipette. This is located on the calibration certificate called “Pipette Calibration Report”... -
Page 30: Easy" Cal Mode
Calibration Note: Ensure that the distilled water used is at room temperature (20-22.5 °C). An internal correction factor of 1.003 is used by the pipette to convert weight (gm) to volume (ml). 2. Calculate an average for the ten readings of distilled water obtained from the balance. -
Page 31: Calview
Calibration 4. Press the [Vol] key to display “CALC” (pressing the [Vol] key again will return you to the “EASY” mode). 5. Press the [Enter] key to display the calibration point. 6. Press the [Vol] key to enter the Display Volume (Dv) calculated above in step 2. -
Page 32: Calreset
Calibration D.2.5 CALRESET CALRESET allows you to return to a default calibration step. To reset the calibration, enter the CAL mode using the Special Function key. When the calibration point is displayed, press the [Purge] key. The display flashes “FACT,” “CAL,” then “SET” and the pipette resets to the default step. This default setting is an average value for all pipettes of its class. - Page 33 Calibration • Pause consistently. It takes a moment for the liquid in the tip to finish moving. Pause with the tip in the liquid for about one second after aspirating the sample to allow the liquid to finish moving into the pipette tip after the pipette stops.
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Page 34: Troubleshooting
Troubleshooting E. TROUBLESHOOTING Possible procedural and system problems, as well as recommended solutions. If you cannot resolve the problem, call technical service at 800-345-0206. No power to the pipette. Probable Cause Recommended Solution Battery is discharged. Charge battery for at least one hour. Double beep tone occurs. - Page 35 Troubleshooting The motor sounds rough and aspiration is very slow. Probable Cause Recommended Solution The pipette has been set to a Verify speed setting, the slower speed slower speed. settings causes the motor to run slowly and louder than usual. Motor has not been used for an Cycle the unit (fill and dispense) five to extended period.
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Page 36: Maintenance
Maintenance F. MAINTENANCE The Equalizer pipette has been specifically designed for serviceability, enabling the user to service instruments with relative ease. Under certain circumstances where on-site service is not feasible, the instrument may need to be returned to Thermo Fisher Scientific for repair (see Pipette Service, Sections G.2 and G3). -
Page 37: Equalizer Pipette Maintenance
Maintenance F.1 Equalizer Pipette Maintenance F.1.1 Equalizer Pipette Disassembly 1. Remove the color ring by gently prying loose one of the edges near the handle trigger. 2. Use a Phillips-head screwdriver to remove all three housing screws. 3. Carefully remove the plastic cover housing and observe the layout of the internal parts. - Page 38 Maintenance 4. Remove the ejector comb by sliding it down and forward off the tip fittings (see Figure F.1.1b). Remove the plastic ejector trigger and spring. Next, remove the back plane divider. Figure F.1.1b: Removing Ejector Trigger and Ejector Comb 5.
- Page 39 Maintenance Figure F.1.1d: Pipette Components Item Description Item Description Keypad Tip fitting Main board Console cover Flex circuit Console display Battery box Battery Driver board Main housing Motor Trigger hinge pin Color ring Handle trigger Motor coupling Handle trigger spring Motor screw Ejector trigger Piston backplate...
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Page 40: Equalizer Pipette Cleaning And Autoclaving
Maintenance F.1.2 Equalizer Pipette Cleaning and Autoclaving 1. Remove the piston assembly from the cylinder. 2. Remove and discard the cross tubes into a biohazardous container. 3. Remove the O-rings (only if necessary, see section F.1.3 for O-ring Replacement) Figure F.1.2: Components to be Autoclaved and discard them into a biohazardous container. - Page 41 Maintenance 2. Install new O-rings. After the old O-rings have been removed and all components have been autoclaved, the new O-rings may be installed. Before installing the O-rings, apply a thin coat of lubricant to each O-ring, using the O-ring lubricant supplied in the Preventive Maintenance/Autoclave Kit.
- Page 42 Maintenance • 125 μl, 30 μl, 12.5 μl Pipettes: Spread the lubricant directly onto the pistons, approximately 0.5 inches (approx. 1.3 cm) from the bottom of each piston (see Figure F.1.3c). Figure F.1.3c: Re-lubricating 125 - 12.5 μl Pipettes F.1.4 Equalizer Pipette Reassembly 1.
- Page 43 Maintenance Tip Fitting Positions 12-Channel 8-Channel Table F.1.4: Cylinders Figure F.1.4b: Cylinder/Cross Tube Positions Note: If the cross tube orientation between the cylinder and tip fittings is incorrect, reassembly of the pipette will be impaired. 3. Installing Piston/Cylinder Assembly Carefully insert the flat end of the motor screw into the hole of the motor coupling.
- Page 44 Maintenance Figure F.1.4c: Installing Piston/Cylinder Assembly 4. Rotating Manifold Assembly After installing the piston/cylinder assembly, install the manifold assembly into the main housing. Make sure manifold is in closed position. To keep the tubing free from ejector contact, rotate the manifold plate and tubing one complete turn counterclockwise (see Figure F.1.4d).
- Page 45 Maintenance Optional Dowel Back Plane Manifold Divider Assembly Figure F.1.4e: Installing Manifold Assembly Note: Use a dowel to line up the screw hole of the main housing with the back plane divider. This will help with reassembly of the main housing and housing cover. 6.
- Page 46 Maintenance Ejector Trigger Figure F.1.4f: Inserting Ejector Trigger 7. Replacing Ejector Comb Slide the ejector comb over the tip fittings with the slots on the back of the comb exposed (the ribs on the ejector trigger should mate with the slots on the aluminum ejector comb - see Figure F.1.3g).
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Page 47: Installing/Replacing Batteries
Maintenance 8. Replacing the Housing Cover Line up the screw hole of the back plane divider with the main housing (using the small dowel works best) and attach the housing cover. Place the cover housing onto the main housing and fasten the three screws. Use dowel to line Housing Cover up screw hole... -
Page 48: Charging/Recharging Batteries
Maintenance F.2 Installing/Replacing Lithium-Ion Battery All Matrix Electronic Equalizer pipettes are now shipped with a Lithium- Ion battery. Before using the pipette, charge it for 2 hours. Use the following procedure when installing a new battery pack. Otherwise, continue to Section F.3, Recharging Batteries. - Page 49 Maintenance 3. Carefully Remove Console Cover. Press and lift off console cover assembly (see Figure F.2e). BE CAREFUL not to tear the attached flex cable! (Refer to Figure F.2f). Figure F.2e: Remove Console Cover 4. Unclasp Battery Connector. Lift one side of battery connector with a small flathead screwdriver.
- Page 50 Maintenance F.3 Charging/Recharging Lithium-Ion Battery The Matrix Equalizer electronic pipette uses a Lithium-Ion rechargeable battery located in a battery case under the keypad. The battery is charged while it is in the pipette by using the power transformer included in the package.
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Page 51: Technical Service
Technical Service G. TECHNICAL SERVICE G.1 Instrument Warranty This instrument is warranted to the original purchaser by Thermo Fisher Scientific to be free of defects in materials or workmanship for a period of 12 months from the date of purchase, providing that is has been operated according to the instructions, not abused or misused, that the serial number has not been removed, and that the instrument has not been disassembled, other than for procedures for normal maintenance. -
Page 52: Recommended Equipment Decontamination Methods
Technical Service G.2 Recommended Equipment Decontamination Methods The following table lists the proper decontamination procedures for returning Matrix Electronic pipettes to Thermo Fisher Scientific for service. Contact customer service at 800-345-0206 (US) or +44(0) 161 486 2112 (Europe) for a current equipment decontamination verification form. Heat at Autoclave 65 °C/... -
Page 53: Factory Service - Calibration, Maintenance, & Repair
Expert Pipette Service Trusted Service Certified technicians calibrate and repair Thermo Scientific Matrix pipettes as well as most other pipette brands and models. With more than 20 years experience manufacturing and servicing pipettes, we offer a broad range of services to fit every need: basic calibrations, preventive maintenance and repair services. -
Page 54: On-Site Site Service - Calibration & Maintenance
Technical Service G.4 On-site Pipette Service Options Services include: • Physical pipette inspection • Leak test • Replacement of seal and O-ring, as needed • Gravimetric performance validation using NIST-traceable balance • Performance validation of five aliquots at two volumes •... -
Page 55: Regulatory Information
Technical Service G.5 Regulatory Information... - Page 56 Index INDEX Autoclaving Maintenance Automatic blowout 6,15 Maintenance Procedures 31-45 Batteries, Charging (Li Ion) Mix Key Batteries, Installation (Li Ion) Battery Case 4,44 On/Off Key Open Programming Mode Operation Keys “CALC” Mode Calibration (Procedures) 23-28 PACE Calibration (“CALC” Mode) Paced Dispense 8,22 Calibration (“EASY”...
- Page 57 Index...
- Page 58 Thermo Fisher Scientific, Inc. 81 Wyman Street P.O. Box 9046 Waltham, Massachusetts 02454-9046 United States Thermo Fisher Scientific Matrix Liquid Handling Products 22 Friars Drive Hudson, New Hampshire 03051 United States Technical Support: North America Tel: 800.345.0206 Email: ushud-technicalservices@thermofisher.com Hours of Operation: 8:00 AM to 5:00 PM ET Europe Tel: +44(0) 161 486 2112 Email: matrixtechcorp.eu.service@thermofisher.com...
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