Chapter 3 Create A Planned Run; About Planned Runs; Create A Planned Run - Thermo Scientific Ion Torrent S5 User Manual

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About planned runs

Create a planned run

Ion Torrent S5 ™ andIon Torrent S5
CONFIDENTIAL — For Life Technologies Internal Use Only. Do Not Distribute.
IMPORTANT! This sequencing kit is compatible with Ion Torrent
later. Before proceeding, check for updates to the Ion Torrent
Torrent S5 ™ or Ion Torrent S5
available.
Note: If you are currently using Ion Reporter
of the Torrent Suite ™ Software, contact Technical Support for assistance with
upgrading to Ion Torrent
Planned runs contain all the settings used in a sequencing run, including number of
flows, kit types, barcodes used (if any), run type (e.g., DNA, RNA, amplicons), and
reference file (if any). They provide a fast and convenient way to set up and organize
your runs.
You create planned runs using the Torrent Browser and then select the appropriate
plan in the Select Planned Run screen of the Ion Torrent S5
Sequencer when you are ready to perform the run (see t_Start the sequencing run).
Note: For additional information, see the Torrent Suite
on the Ion Community at ioncommunity.lifetechnologies.com.
1. Open Torrent Browser for the Ion Torrent
S5 ™ or Ion Torrent S5
2. Select the Plan tab, select Templates, locate the application you want to run (for
example, AmpliSeq DNA), then select:
• Upload Plans to upload a .csv format file containing your run information.
• Plan New Run to plan a new run using the generic template for the selected
application.
• Add New Template to create a new planned run template that you can
reuse for the selected application.
• Other options that may be available depending on your selected application,
such as downloading designs from AmpliSeq.com.
3. When you have completed your selections, click Plan Run to save your
selections. The run is listed on the Planned Runs page under the name you
specified.
™ XL Instrument User Guide
Create a Planned Run
™ XL Sequencer software, and install the updates if
™
Software v1.4 with an earlier version
™ System 4.4.
™ Server connected to your Ion Torrent
™ XL Sequencer .
™
System 4.4 and
™
System and Ion
™ ™
or Ion Torrent S5
™ User Interface Guide, available
XL
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