Chemiluminescence (Chemi) Mode; Camera Settings; Interactive Chemi - Thermo Scientific myECL Imager Manual

Chemiluminescence Chemiluminescence (Chemi) Mode is the default mode. Chemi mode is used for the
capture of sample images using sample-emitted light. A visible image of the sample
(Chemi) Mode
is automatically captured in Chemi mode using reflected light (ChemiV image), which
is useful for imaging colorimetric molecular weight markers.

Camera Settings

Focus, Digital Zoom and Resolution/Sensitivity camera settings are available in
Chemi mode.

Interactive Chemi

Interactive Chemi is a patent-pending interactive tool used to evaluate and optimize
imaging exposure time before acquiring a Western blot image. The Interactive Chemi
button automatically calculates the exposure time of the Western blot with maximum
dynamic range and minimal pixel saturation from a short exposure image. The
feature displays real-time changes of the calculated Western blot image with
adjusted exposure time and allows further exposure-time refinement through user
input.
1. Select the Interactive Chemi button. The imager acquires a 15-second
exposure.
Note A Stop button appears over the keypad area once the Interactive Chemi button
is selected. Image acquisition can be stopped by selecting the Stop button.
30 ECL Imager User Manual Thermo Fisher Scientific
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