Setting Up The Method - Agilent Technologies 1290 Infinity LC System System Manual And Quick Reference

5
Quick Start Guide
Data Acquisition in Method and Run Control View

Setting Up the Method

This section shows how to quickly set the method conditions for an analysis
using the test mixture conditions. For a more detailed explanation of all the
available parameters see Appendix,
Method"
The default method DEF_LC.M has been loaded ready to prepare the new method.
1 To quickly access the Method page for each module, right-click in the system
2 Right-click the pump area, and select Method... in the context menu.
3 Right-click the autosampler area, and select Method... in the context menu.
98
on page 115.
Now the key parameters can be edited to create the new method. For
the example separation discussed here, set the conditions listed in
on page 97.
Table 9
diagram for the module and select Method... from the context menu.
Each of the modules will be set up in this way.
a In the Method page for the 1290 Infinity Binary Pump, enter the following
parameters:
•
Flow rate: 0.4 ml/min
• Solvent A: Select Water from the compressibility drop-down list.
•
Solvent B: Select the check box to make Solvent B active.
•
%B: Initial value 40 %
•
Stop Time: 5 min
•
Max Pressure Limit: 1200 bar
b Click the + sign to open the Timetable.
c Add a line, select Change Solvent Composition, and set %B to 80 %
d Other parameters can remain at default settings. Click OK to exit the
window.
The changes are sent to the pump module.
a In the Method page for the 1290 Infinity Autosampler, enter the following
parameters:
•
Injection volume: 1.0 µl
•
Injection with Needle Wash
•
Mode Flush Port, Time: 6 s
"Setting Up a Method using Edit Entire
Agilent 1290 Infinity LC System Manual and Quick Reference