Agilent Technologies 1290 Infinity LC System System Manual And Quick Reference page 43

Table 4
System Configurations
Binary Pump + Fixed Loop Autosampler (MS only)
Binary Pump + Jet Weaver + Fixed Loop
Binary Pump + standard Autosampler (MS only)
Binary Pump + Jet Weaver + Autosampler
1
Table 5
System Configurations
Quaternary Pump + Fixed Loop Autosampler (MS only)
Quaternary Pump + standard Autosampler (MS only)
Quaternary Pump + V380 Jet Weaver + Fixed Loop
Quaternary Pump + V380 Jet Weaver + Autosampler
1
Table 6
Flow
Rate
(ml/min)
0.2
0.4
0.6
0.8
1.0
Agilent 1290 Infinity LC System Manual and Quick Reference
Delay volumes of 1290 Infinity Binary LC system configurations
1
added 5 µl to allow for connections in system configurations
Delay volumes of 1290 Infinity Quaternary LC system configurations
1
added 5 µl to allow for connections in system configurations
System delay times for gradient to reach head of column
20 55
0.10 0.28
0.05 0.14
0.03 0.09
0.03 0.07
0.02 0.06
Optimization of the Agilent 1290 Infinity LC System
How to Configure the Optimum Delay Volume
System Delay Volume (microliters)
90 125 360
Delay Times (minutes)
0.43 0.60 1.80
0.21 0.30 0.90
0.14 0.20 0.60
0.11 0.15 0.45
0.09 0.12 0.36
Delay Volume (µl)
20
55
90
125
Delay Volume (µl)
360
430
510
580
395 430 465
2.15 2.55 2.90
1.08 1.28 1.45
0.72 0.85 0.97
0.54 0.64 0.73
0.43 0.51 0.58
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