Agilent Technologies 1290 Infinity LC System System Manual And Quick Reference page 126

6
Appendix
Setting Up a Method using Edit Entire Method
Thermostatted Column Compartment (TCC) tab
Figure 40
• Temperature defines the temperature of the left and right-hand side column
126
Setup Method screen – Thermostatted Column Compartment tab
holders which can be independently controlled or linked together by
clicking the Combined radio button on. When combined the settings for the
left side control both sections and this is certainly necessary when the
column is longer than 15 cm and needs to be supported by both sections.
The two sides can be operated separately when it is required to have two
columns operating at different temperatures – this can be implemented
when a column switching valve is also installed to switch between them.
Another use of separate temperature zones is when the column is operated
at a high temperature (e.g. above 60 °C) on one side and the heat exchanger
on the other side is used to cool the eluent before it enters the detector
thereby reducing noise due to thermal effects in the flow cell. Selecting the
As Detector Cell option will automatically read the temperature from the cell
in the 1290 Infinity Detector.
Agilent 1290 Infinity LC System Manual and Quick Reference